Purpose
Contains a synthetic DNA sequence, VAR1u, that specifies the Var1 protein. The Cox4 targeting signal is fused to the N-terminus to direct import of Var1. Complements var1 mutations in mtDNA.
Insert
VAR1u, VAR1 recoded for expression from the nucleus
Use
Yeast ecoli shuttle vector
Tags
preCox4 mitochondrial matrix targeting signal
Expression
Yeast
Mutation
Synthetic gene in standard genetic code
Available since
Aug. 15, 2014
Availability
Academic Institutions and Nonprofits only