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pETM6_Ptrc_BsMdh_BmHps_BmPhi

Plasmid

#129104

Purpose

Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus

Depositor

Eleftherios Terry Papoutsakis

Article

Bennett et al Metab Eng. 2018 Jan;45:75-85. doi: 10.10

Insert

BsMDH BmHPS BmPHI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA4/TO-CDC25A

Plasmid

#216511

Purpose

Expresses CDC25A in human cells under a tetracycline/doxycycline-inducible promoter

Depositor

John Diffley

Article

Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

Insert

CDC25A (CDC25A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pACYC Destination

Plasmid

#207675

Purpose

Trio “destination vectors” which harbor an operon for constitutive expression of the repressors for each inducible promoter (CymR, PhlF, and VanR for cumate, DAPG and vanillate, respectively).

Depositor

Ramon Gonzalez

Article

Lee et al Metab Eng. 2024 Mar;82:262-273. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

CymRC, PhlF, VanCC

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pTE1069

Plasmid

#186629

Purpose

SCB-GFP E. coli reporter plasmid. Encodes repressor ScbR and ScbAp promoter upstream of GFP.

Depositor

Eriko Takano

Article

Biarnes-Carrera et al ACS Synth Biol. 2018 Apr 20;7(4):1043-10

Inserts

scbR
scbAp promoter

Use

Synthetic Biology

Tags

6xArg

Expression

Bacterial

Mutation

Promoter

BBa_J23100 and scbAp

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pET303-ColE1-TR-del1-40

Plasmid

#180236

Purpose

Expresses T and R domains of Colicin E1 with N-terminal 40 residue deletion (residues 41-364)

Depositor

Joanna Slusky

Article

Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

Insert

Colicin E1

Use

Tags

6x His-tag

Expression

Bacterial

Mutation

Truncation of ColE1 containing T and R domains (r…

Promoter

T7

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pET303-ColE1-T-P110A

Plasmid

#180237

Purpose

Expresses T domain of Colicin E1 (residues 1-190) with proline to alanine mutation at residue 110

Depositor

Joanna Slusky

Article

Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

Insert

Colicin E1

Use

Tags

6x His-tag

Expression

Bacterial

Mutation

Truncation of ColE1 containing T domain of ColE1 …

Promoter

T7

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_PDCD6IP_ALIX V-domain

Plasmid

#178483

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

PDCD6IP_ALIX V-domain (PDCD6IP Human, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_YES1_SH3

Plasmid

#178492

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

YES1_SH3 (YES1 Human, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_WDR5_WD

Plasmid

#178491

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

WDR5_WD (WDR5 Human, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTH32 [pmyo-3::YFP-CaCyclOp(A-2x)::SL2::mCherry]

Plasmid

#168168

Purpose

Expression of YFP-CaCyclOp(A-2x) in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

YFP-CaCyclOp(A-2x)

Use

Tags

YFP

Expression

Worm

Mutation

E497K and C566D

Promoter

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pTH42 [punc-17::YFP-CaCyclOp(A-2x)::SL2::mCherry]

Plasmid

#168171

Purpose

Expression of YFP-CaCyclOp(A-2x) in cholinergic motor neurons of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

YFP-CaCyclOp(A-2x)

Use

Tags

YFP

Expression

Worm

Mutation

Promoter

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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csd5_41-192/pET28b(+)

Plasmid

#83296

Purpose

csd5, construct: 41-192, pET28b(+) N-tag

Depositor

SeWon Suh

Article

Kim et al Acta Crystallogr D Biol Crystallogr. 201

Insert

hp1250

Use

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-40

Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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csd4_22-438/pET28b(+)

Plasmid

#83295

Purpose

csd4, construct: 22-438, pET28b(+) N-tag

Depositor

SeWon Suh

Article

Kim et al Acta Crystallogr D Biol Crystallogr. 201

Insert

hp1075

Use

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-21

Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only
p414GPD-3xFLAG-RBM28 WT

Plasmid

#169265

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 WT

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 WT (RBM28 Human)

Use

Tags

3xFLAG

Expression

Yeast

Mutation

Promoter

GPD

Available since

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO - RBM28 E8 WT minigene

Plasmid

#169271

Purpose

Mammalian vector for constitutive expression of RBM28 Exon 8 WT minigene

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 E8 WT minigene (RBM28 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Availability

Academic Institutions and Nonprofits only

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pDA095

Plasmid

#131391

Purpose

generation of Tet-On RPE

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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Zebrabow-GateDest

Plasmid

#118221

Purpose

For cloning promoter into a Zebrabow vector with Gateway reaction. Only compatible with single site Gateway. Not compatible with Tol2-kit.

Depositor

Alex Schier, Albert Pan

Article

Pan et al Development. 2013 Jul;140(13):2835-46. d

Insert

Brainbow 1.0 L - ccdB survival cassette

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pETM33_SUFU_Sufu

Plasmid

#178488

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

SUFU_Sufu (SUFU Synthetic, Human)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLTR.gp140/EGFP.Revdelta38/DsRed

Plasmid

#115775

Purpose

HIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.

Depositor

Damian Purcell

Article

Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

Insert

Envelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).

Use

Tags

EGFP; dsReD

Expression

Mammalian

Mutation

gp140 uncleaved (1190-91aa, KR > TG; truncated…

Promoter

HIV-1 (LTR)

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pDA034

Plasmid

#131386

Purpose

ORFeus expression, mutant

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Use

Tags

ORF2-3xFlag

Expression

Mammalian

Mutation

D702Y RT

Promoter

Available since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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