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pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A
Plasmid#51248PurposeEncodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to AlaDepositorInsertGli3 (Gli3 Mouse)
UseFlpin systemTags3xHA-TEV and FlagExpressionMammalianMutationamino acids 849,865,877,907,980,1006 mutated to A…PromoterEF1aAvailable sinceMarch 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT/DEST-3xHA/mGli2 Pc-gA/P1-6A
Plasmid#51257PurposeEncodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248,789,805,817,848,923,956 mutated to AlaDepositorInsertGli2 (Gli2 Mouse)
UseFlpin systemTags3xHA-TEVExpressionMammalianMutationamino acids 216,220,224,230,248,789,805,817,848,9…PromoterEF1aAvailable sinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT/DEST-3xHA/mGli3/Flag P5-6A
Plasmid#51249PurposeEncodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 980,1006 mutated to AlaDepositorInsertGli3 (Gli3 Mouse)
UseFlpin systemTags3xHA-TEV and FlagExpressionMammalianMutationamino acids 980,1006 mutated to AlaPromoterEF1aAvailable sinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT/DEST-3xHA/mGli3/Flag P1-4A
Plasmid#51247PurposeEncodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907 mutated to AlaDepositorInsertGli3 (Gli3 Mouse)
UseFlpin systemTags3xHA-TEV and FlagExpressionMammalianMutationamino acids 849,865,877,907 mutated to AlaPromoterEF1aAvailable sinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
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KARS_pNIC-Bio3
Plasmid#153051PurposeExpression construct for producing Avi tagged Lysyl-tRNA synthetaseDepositorInsertKARS (KARS1 Human)
UseTagsAvi and His6-TEVExpressionBacterialMutationPromoterT7Available sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
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TBXTA-c043
Plasmid#139766PurposeProtein expression/Streptavidin pull-downDepositorInsertTBXT, DNA-binding domain, WT (TBXT Human)
UseTagsAvi-tag (Biotin) and His6-TEVExpressionBacterialMutationContains only amino acids E41- D225PromoterT7Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCI-Puro_hCOL4A6-Myc-His
Plasmid#198087PurposeExpress hCOL4A6 in mammalian cellsDepositorInsertCOL4A6 (COL4A6 Human)
UseTagsTEV-Myc-HisExpressionMammalianMutationPromoterCMV, beta-globin/IgG chimeric intronAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
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TBC1D7
Plasmid#32047PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTBC1D7 (TBC1D7 Human)
UseTagsHis (with TEV cleavage)ExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
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5VBD (USP09X)
Plasmid#101260PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertUSP09X (USP9X Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
