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KHBD00016
Plasmid#34178DepositorInsertCG8361 (E(spl)m7-HLH Fly)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJan. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pDONR221-hSOX10-G1237A
Plasmid#24733DepositorInsert(sex determining region Y)-box 10 (SOX10 Human)
UseGateway donor vectorTagsExpressionMutationG replaced with A at 1237 position resulting in G…PromoterAvailable sinceMay 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR-hSOX10-G128A
Plasmid#24737DepositorInsert(sex determining region Y)-box 10 (SOX10 Human)
UseGateway donor vectorTagsExpressionMutationG replaced with A at position 128PromoterAvailable sinceMay 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-hSOX10-G1238A
Plasmid#24738DepositorInsert(sex determining region Y)-box 10 (SOX10 Human)
UseGateway donor vectorTagsExpressionMutationG replaced with A at position 1238 resulting in G…PromoterAvailable sinceMay 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pInt-ERCreER
Plasmid#190040PurposeThis is an integration donor plasmid to insert a DNA fragment for doxycycline inducible ERT2-Cre-ERT2 at the AAVS1 locus. It works with an AAVS1 targeting CRISPR/Cas9 construct (Addgene #72833).DepositorInsertERT2-Cre-ERT2 flanked by AAVS1
UseCRISPR and TALENTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Tet-OsTIR1(F74G)-V5
Plasmid#158664PurposeTet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locusDepositorInsertTet-OsTIR1(F74G) AAVS1
UseCRISPRTagsExpressionMammalianMutationF74GPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMH015_AAVS1_puro-pA-core-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry
Plasmid#179437PurposeDual fluorescent reporter construct with double core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locusDepositorInsertcoreHS4-TRE-cit-coreHS4-mch (DC)
UseTagsExpressionMammalianMutationPromoterpEF alpha, pRSVAvailable sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC13N-iCAG.copGFP
Plasmid#66578PurposeCLYBL safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-C13 TALENsDepositorInsertcopGFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS-mEmerald-VIM
Plasmid#62849PurposeDonor vector for gene editing at human VIM locusDepositorInsertmEmerald-VIM (VIM Synthetic, Human)
UseYeast-e.coli shuttle vectorTagsExpressionMutationPromoterAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pODN-mNG-ECT2
Plasmid#183835PurposeRepair template for the N-terminal tagging of Ect2 with mNeonGreen in human cells using CRISPR/Cas9.DepositorInsertECT2 homology arms with mNeonGreen-linker (ECT2 Human)
UseCRISPR; Donor templateTagsmNeonGreen-linkerExpressionMammalianMutationHomology arms contain point mutations to remove t…PromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-iPE-Plus
Plasmid#214062Purposeinducible PE-Plus system for controllable prime editing; this plasmid is used to insert PE-Plus prime editor (PEmax-P2A-hP53DD-IRES-MLH1dn) in one allele of AAVS1 locusDepositorInsertPE-Plus
UseTagsExpressionMammalianMutationPromoterTRE-tightAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAT-PB-CD2APtk
Plasmid#86004Purposehomology donor for AAT locus with piggyBac-flanked DsRedEx_T2A_puroTK cassetteDepositorInsertDsRedEx_T2A_puro_dTK
UsePiggybac exciseableTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
HR180-LGR5-iCT
Plasmid#129094PurposeKI iCaspase9-tdTomato with a puromycin/mRuby selection cassette into human LGR5 locusDepositorInsertiCaspase9-T2A-tdTomato and LGR5 homology arms (LGR5 Human)
UseHomologous recombination donor plasmidTagsExpressionMutationPromoterAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19-OCT4-T2A-NLS-EmGFP-P2A-Puro
Plasmid#89992PurposeDonor template for generation of OCT4-T2A-nEmGFP reporter cell linesDepositorInsertOCT4-T2A-NLS-EmGFP-P2A-Puro (POU5F1 Human)
UseCRISPRTagsExpressionMutationPromoternoneAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
Intron-Tagging-EGFP-Frame0-Parental-Minicircle
Plasmid#216124PurposeParental minicircle containing EGFP flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the EGFP tag as a synthetic exon into frame 0 introns of target genes.DepositorInsertsgRNA-SA-(GGGGS)x4-EGFP-(GGGGS)x4-SD
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Puro_siKD
Plasmid#86695PurposeTetracycline inducible expression of shRNA targeted to AAVS1 locusDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterH1 TOAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19/PLIN2-GFP
Plasmid#202194Purposeknock-in donor vector of GFP to the C-terminus of endogenous human PLIN2DepositorInsertPLIN2 (PLIN2 Human)
UseCloning vectorTagsGFP knock-inExpressionMutationPromoterlacAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only