Addgene

We narrowed to 3,970 results for: KRAS

Showing: 2521 - 2540 of 3970 results

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pTE4999

Plasmid

#107555

Purpose

Expresses human codon optimized inactive FnCpf1(dead1) in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

hFnCpf1(dead1)

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

D917A

Promoter

CMV

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pKK-FLAG-TEV

Plasmid

#105768

Purpose

Expression of your protein of interest in fusion with FLAG at the N-terminus. The tag is cleavable by TEV protease or enterokinase.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

FLAG-TEV

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-NR1H2

Plasmid

#192919

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of NR1H2

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

NR1H2 (NR1H2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

TET3G-miniCMV

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-ZFPM2

Plasmid

#192922

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of ZFPM2

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

ZFPM2 (ZFPM2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

TET3G-miniCMV

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pKK-TEV-mEGFP

Plasmid

#105794

Purpose

Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pTE4565

Plasmid

#88903

Purpose

Expresses inactive/dead, humanized MbCpf1 nuclease

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

hMbCpf1(D986A)

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTE4567

Plasmid

#107557

Purpose

Expresses human codon optimized inactive MbCpf1(dead2) in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

hMbCpf1(dead2)

Use

Tags

3xHA and NLS

Expression

Mammalian

Mutation

E1080A

Promoter

CMV

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 sgATXN1L-1

Plasmid

#74970

Purpose

sgATXN1L-1, puromycin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ATXN1L (ATXN1L Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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hCXCR4-mTQ2

Plasmid

#110196

Purpose

Encodes for human CXCR4 coding sequence tagged with the mTQ2 FP

Depositor

Sergi Padilla Parra

Article

Starling et al Nat Commun. 2023 Aug 17;14(1):4994. doi:

Insert

hCXCR4 (CXCR4 Human)

Use

Tags

mTurquoise (mTQ2)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGria3

Plasmid

#124869

Purpose

Mutagenesis of Gria3

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gria3 (Gria3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 sgCIC-2

Plasmid

#74967

Purpose

sgCIC-2, puromycin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

CIC (CIC Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGAS R71/75E C396/7A-HA

Plasmid

#130920

Purpose

Expresses human cGAS with R71/75E and C396/397A point mutations; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

human cGAS R71/75E C396/7A

Use

Tags

HA

Expression

Mammalian

Mutation

aa 71 and 75 mutated to glutamate; aa 396 and 397…

Promoter

CMV

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 p37 SHPmut

Plasmid

#113503

Purpose

Bacterial expression of GST-tagged p37 that is p97 binding deficient due to mutations in the SHP box

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Use

Tags

GST-PreScission protease site

Expression

Bacterial

Mutation

F215A/E218A/QKL220-222AAA

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pTE3320

Plasmid

#107529

Purpose

Expresses human codon optimized LbCpf1(RVR mutant) in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

hLbCpf1(RVR)

Use

Tags

3xHA and NLS

Expression

Mammalian

Mutation

G532R, K538V, Y542R

Promoter

CMV

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pTE4561

Plasmid

#107525

Purpose

Expresses Lb crRNA and mCherry in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Lb crRNA
mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CBh and human U6

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 sgATXN1L-2

Plasmid

#74971

Purpose

sgATXN1L-2, puromycin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ATXN1L (ATXN1L Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 p37 deltaUBX

Plasmid

#113504

Purpose

Bacterial expression of GST-tagged p37 lacking the UBX domain

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Use

Tags

GST-PreScission protease site

Expression

Bacterial

Mutation

delta 251-331 (stop codon behind AA250)

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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NFATc2-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75234

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc2 (1/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc2-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75235

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc2 (2/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pKK-mVenus-TEV

Plasmid

#105774

Purpose

Expression of your protein of interest in fusion with yellow fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

mVenus-TEV

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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