Addgene

We narrowed to 13,574 results for: pET

Showing: 2501 - 2520 of 13574 results

Results per page:

Sort results by:

pET-20b-T7-Ntx

Plasmid

#58714

Purpose

Expresses T7-Notexin in Escherichia coli

Depositor

Fiorella Tonello

Article

Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

Insert

Notexin

Use

Tags

T7 peptide tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pET14b-PITPalpha N90F

Plasmid

#58257

Purpose

bacterial expression of human PITP alpha N90F

Depositor

Shamshad Cockcroft

Article

Tilley et al Structure. 2004 Feb;12(2):317-26.

Insert

PITP alpha (PITPNA Human)

Use

Tags

HIS

Expression

Bacterial

Mutation

N90F (abolishes PI binding)

Promoter

T7

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
pET14b-PITPalpha K61A

Plasmid

#58258

Purpose

bacterial expression of human PITP alpha K61A

Depositor

Shamshad Cockcroft

Article

Tilley et al Structure. 2004 Feb;12(2):317-26.

Insert

PITP alpha (PITPNA Human)

Use

Tags

HIS

Expression

Bacterial

Mutation

K61A (abolishes PI binding)

Promoter

T7

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
pET29b-ClbP-S95A

Plasmid

#48245

Purpose

Untagged, full-length protein with active site mutation Serine95 to Alanine for in vivo peptidase assays

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbP

Use

Tags

Expression

Bacterial

Mutation

Changed Serine 95 to Alanine

Promoter

T7

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-28a-CylI

Plasmid

#48200

Purpose

Expresses CylI (type III PKS) in E. coli

Depositor

Emily Balskus

Article

Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

Insert

CylI

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-29b-CylB

Plasmid

#48199

Purpose

Expresses CylB (acyl carrier protein) in E. coli

Depositor

Emily Balskus

Article

Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

Insert

CylB

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pET20b- hTPI Ile170Thr

Plasmid

#50725

Purpose

overexpression of the human TPI1 Ile170Thr allele in E.coli

Depositor

Markus Ralser

Article

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Use

Tags

6xHIS tag

Expression

Bacterial

Mutation

changed Isoleucine 170 to Threonine

Promoter

T7

Available since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pET12a-AL-103 delProIns

Plasmid

#47095

Depositor

Marina Ramirez-Alvarado

Insert

AL-103 delProIns (IGKV3-20 Human)

Use

Tags

ompT

Expression

Bacterial

Mutation

delete aa95

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET12a-AL-103 Y96F

Plasmid

#47094

Depositor

Marina Ramirez-Alvarado

Article

DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

Insert

AL-103 Y96F (IGKV3-20 Human)

Use

Tags

ompT

Expression

Bacterial

Mutation

Y96F

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET12a-AL-103 Y32F

Plasmid

#47091

Depositor

Marina Ramirez-Alvarado

Article

DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

Insert

AL-103 Y32F (IGKV3-20 Human)

Use

Tags

ompT

Expression

Bacterial

Mutation

Y32F

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET12a-AL-09 FL Q42K C214S

Plasmid

#47089

Depositor

Marina Ramirez-Alvarado

Article

Levinson et al Sci Rep. 2013;3:1278. doi: 10.1038/srep0

Insert

AL-09 FL Q42K C214S (IGKV3-20 Human)

Use

Tags

ompT

Expression

Bacterial

Mutation

Q42K C214S

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET12a-AL-09 FL I34N C214S

Plasmid

#47088

Depositor

Marina Ramirez-Alvarado

Article

Levinson et al Sci Rep. 2013;3:1278. doi: 10.1038/srep0

Insert

AL-09 FL I34N C214S (IGKV3-20 Human)

Use

Tags

ompT

Expression

Bacterial

Mutation

I34N C214S

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET12a-AL-09 Y32F Y96F

Plasmid

#47083

Depositor

Marina Ramirez-Alvarado

Article

DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

Insert

AL-09 Y32F Y96F (IGKV3-20 Human)

Use

Tags

ompT

Expression

Bacterial

Mutation

Y32F Y96F

Promoter

T7

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only
pET21a(+)-HpDsbG C162A

Plasmid

#39330

Depositor

SeWon Suh

Article

Yoon et al FEBS Lett. 2011 Dec 15;585(24):3862-7. E

Insert

oxidoreductase

Use

Tags

His-tag

Expression

Bacterial

Mutation

changed Cysteine 162 to Alanine

Promoter

T7

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
pET28b(+)-HP0377 C92A

Plasmid

#39335

Depositor

SeWon Suh

Insert

oxidoreductase

Use

Tags

His-tag

Expression

Bacterial

Mutation

changed Cysteine 92 to Alanine

Promoter

T7

Available since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only
pET15b-eIF4E-S200C

Plasmid

#37229

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

eIF4E

Use

Tags

Expression

Bacterial

Mutation

S200C

Promoter

T7

Available since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only
HP0421_(7-383)_pET21a

Plasmid

#29790

Depositor

SeWon Suh

Insert

hp0421 (HP0421 H. pylori)

Use

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 1-6 and 384-389

Promoter

Available since

March 27, 2012

Availability

Academic Institutions and Nonprofits only
pETCON-HB28/design_25

Plasmid

#30260

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB28

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-E43/design_55

Plasmid

#30237

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

Mtb ACP2 designed binding protein E43

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
pETCON-HB14/design_53

Plasmid

#30249

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB14

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only

Showing: 2501 - 2520 of 13574 results

Results per page:

Sort results by:

pET-20b-T7-Ntx

pET14b-PITPalpha N90F

pET14b-PITPalpha K61A

pET29b-ClbP-S95A

pET-28a-CylI

pET-29b-CylB

pET20b- hTPI Ile170Thr

pET12a-AL-103 delProIns

pET12a-AL-103 Y96F

pET12a-AL-103 Y32F

pET12a-AL-09 FL Q42K C214S

pET12a-AL-09 FL I34N C214S

pET12a-AL-09 Y32F Y96F

pET21a(+)-HpDsbG C162A

pET28b(+)-HP0377 C92A

pET15b-eIF4E-S200C

HP0421_(7-383)_pET21a

pETCON-HB28/design_25

pETCON-E43/design_55

pETCON-HB14/design_53

Help Center

Deposit Plasmids

General Reagents

Viral Service

DNA Service

Plasmid Collections