Addgene

We narrowed to 5,234 results for: pCas

Showing: 2501 - 2520 of 5234 results

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pBW2609_pCAG-PV1-PYL-L1-iCre-252C-NLS-BGHpA

Plasmid

#108742

Purpose

Expresses aa252 to C-terminus of Cre recombinase fused to PYL CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment aa252 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

NLS and PYL

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2164_pCAG-PV1-FlpO-N168-L1-GID1-NLS-BGHpA

Plasmid

#108749

Purpose

Expresses N-terminus to aa168 of Flp recombinase fused to GID1 CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment N-terminus to aa168

Use

Cre/Lox and Synthetic Biology

Tags

GID1,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2184_pCAG-PV1-GAI-L1-FlpO-169C-NLS-BGHpA

Plasmid

#108750

Purpose

Expresses aa169 to C-terminus of Flp recombinase fused to GAI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment aa169 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

GAI and NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2173_pCAG-PV1-FlpO-N374-L1-GID1-NLS-BGHpA

Plasmid

#108751

Purpose

Expresses N-terminus to aa374 of Flp recombinase fused to GID1 CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment N-terminus to aa374

Use

Cre/Lox and Synthetic Biology

Tags

GID1,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2193_pCAG-PV1-GAI-L1-FlpO-375C-NLS-BGHpA

Plasmid

#108752

Purpose

Expresses aa375 to C-terminus of Flp recombinase fused to GAI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment aa375 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

GAI and NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2230_pCAG-PV1-GAI-L1-iCre-252C-NLS-BGHpA

Plasmid

#108726

Purpose

Expresses aa252 to C-terminus of Cre recombinase fused to GAI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment aa252 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

GAI and NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2598_pCAG-PV1-iCre-N229-L1-FRB-NLS-BGHpA

Plasmid

#108731

Purpose

Expresses N-terminus to aa229 of Cre recombinase fused to FRB CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa229

Use

Cre/Lox and Synthetic Biology

Tags

FRB,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2606_pCAG-PV1-FKBP-L1-iCre-230C-NLS-BGHpA

Plasmid

#108732

Purpose

Expresses aa230 to C-terminus of Cre recombinase fused to FKBP CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment aa230 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

FKBP and NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2600_pCAG-PV1-iCre-N251-L1-FRB-NLS-BGHpA

Plasmid

#108733

Purpose

Expresses N-terminus to aa251 of Cre recombinase fused to FRB CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa251

Use

Cre/Lox and Synthetic Biology

Tags

FRB,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2608_pCAG-PV1-FKBP-L1-iCre-252C-NLS-BGHpA

Plasmid

#108734

Purpose

Expresses aa252 to C-terminus of Cre recombinase fused to FKBP CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment aa252 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

FKBP and NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2610_pCAG-PV1-FKBP-L1-iCre-257C-NLS-BGHpA

Plasmid

#108736

Purpose

Expresses aa257 to C-terminus of Cre recombinase fused to FKBP CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment aa257 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

FKBP and NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2209_pCAG-PV1-iCre-N229-L1-GID1-NLS-BGHpA

Plasmid

#108723

Purpose

Expresses N-terminus to aa229 of Cre recombinase fused to GID1 CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa229

Use

Cre/Lox and Synthetic Biology

Tags

GID1,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2229_pCAG-PV1-GAI-L1-iCre-230C-NLS-BGHpA

Plasmid

#108724

Purpose

Expresses aa230 to C-terminus of Cre recombinase fused to GAI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment aa230 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

GAI and NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Thy-1-CRISPR-gRNA#1-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124283

Purpose

sgRNA against human Thy-1

Depositor

Katalin Josvay, Csaba Vizler

Insert

Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
Thy-1-CRISPR-gRNA#3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124285

Purpose

sgRNA against human Thy-1

Depositor

Katalin Josvay, Csaba Vizler

Insert

Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
Thy-1-CRISPR-gRNA#2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124284

Purpose

sgRNA against human Thy-1

Depositor

Katalin Josvay, Csaba Vizler

Insert

Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
pJSC176 - Bacterial expression plasmid for SpCas9 + Q926A variant

Plasmid

#101206

Purpose

Bacterial expression plasmid for SpCas9 + Q926A variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant Q926A

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

Q926A

Promoter

T7

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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BPK4393 - human expression plasmid for SpCas9 Cluster 5 + Q926A

Plasmid

#101192

Purpose

Human expression plasmid for SpCas9 Cluster 5 + Q926A variant: CMV-T7-hSpCas9-Cluster5+Q926A(K918A, V922A, R925A, Q926A)-NLS(SV40)-3xFLAG

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

hSpCas9-Cluster5+Q926A(K918A/V922A/R925A/Q926A)

Use

Tags

3x FLAG and NLS (SV40)

Expression

Mammalian

Mutation

K918A/V922A/R925A/Q926A

Promoter

CMV

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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MMW3629 - human expression plasmid for SpCas9 Cluster 3 + Q926A

Plasmid

#101188

Purpose

Human expression plasmid for SpCas9 Cluster 3 + Q926A variant: CMV-T7-hSpCas9-Cluster3+Q926A(T657A, G658A, W659A, R661A, Q926A)-NLS(SV40)-3xFLAG

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

hSpCas9-Cluster3+Q926A(T657A/G658A/W659A/R661A/Q926A)

Use

Tags

3x FLAG and NLS (SV40)

Expression

Mammalian

Mutation

T657A/G658A/W659A/R661A/Q926A

Promoter

CMV

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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MMW3759 - human expression plasmid for SpCas9 Cluster 4 + Q926A

Plasmid

#101190

Purpose

Human expression plasmid for SpCas9 Cluster 4 + Q926A variant: CMV-T7-hSpCas9-Cluster4+Q926A(F491A, M495A, T496A, N497A, Q926A)-NLS(SV40)-3xFLAG

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

hSpCas9-Cluster4+Q926A(F491A/M495A/T496A/N497A/Q926A)

Use

Tags

3x FLAG and NLS (SV40)

Expression

Mammalian

Mutation

F491A/M495A/T496A/N497A/Q926A

Promoter

CMV

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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