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pBlastR-5xQUAS-EBFP2-GMRWhite

Plasmid

#165921

Purpose

Blasticidin resistant 5xQUAS EBFP2 GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable QUAS Response Vector

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-5xQUAS-sfGFP-MiniWhite

Plasmid

#165922

Purpose

Blasticidin resistant 5xQUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable QUAS Response Vector

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-5xQUAS-EBFP2-MiniWhite

Plasmid

#165924

Purpose

Blasticidin resistant 5xQUAS EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable QUAS Response Vector

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-5xUAS-EBFP2-MiniWhite

Plasmid

#165912

Purpose

Blasticidin resistant 5xUAS EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable UAS Response Vector

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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P[acman]-Alpha1

Plasmid

#165858

Purpose

Alpha1 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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P[acman]-Omega1

Plasmid

#165860

Purpose

Omega1 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pR6Kg-Alpha2

Plasmid

#165863

Purpose

Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmLSm3_A

Plasmid

#145888

Purpose

Insect Expression of DmLSm3

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm3 (LSm3 Fly)

Expression

Insect

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-5xUAS-mCherry-GMRWhite

Plasmid

#165908

Purpose

Blasticidin resistant 5xUAS mCherry GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable UAS Response Vector

Use

Synthetic Biology

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-5xQUAS-mCherry-GMRWhite

Plasmid

#165920

Purpose

Blasticidin resistant 5xQUAS mCherry GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable QUAS Response Vector

Use

Synthetic Biology

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-12xLexAOp-sfGFP-MiniWhite

Plasmid

#165916

Purpose

Blasticidin resistant 12xLexAOp sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable LexAOp Response Vector

Use

Synthetic Biology

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-5xQUAS-mCherry-MiniWhite

Plasmid

#165923

Purpose

Blasticidin resistant 5xQUAS mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable QUAS Response Vector

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCbh-SpyCas9-2A-puro-pA_U6-sgRosa26

Plasmid

#149346

Purpose

Mammalian expression vector for SpyCas9, puromycin resistance and mouse Rosa26 guide RNA

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

Cas9

Use

CRISPR

Tags

Flag tag

Expression

Mammalian

Promoter

Cbc

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCbh-SpyCas9-2A-puro-pA_U6-BbsI

Plasmid

#149347

Purpose

Mammalian expression vector for SpyCas9 and puromycin resistance

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

SpyCas9

Use

CRISPR

Tags

Flag tag

Expression

Mammalian

Promoter

Cbh

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCbh-SpyCas9-T2A-puro-pA_U6-sgPolb (pE047)

Plasmid

#149350

Purpose

Mammalian expression vector for SpyCas9, puromycin resistance and human Polb guide RNA

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

SpyCas9

Use

CRISPR

Tags

Flag tag

Expression

Mammalian

Promoter

Cbh

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pTEI126

Plasmid

#126066

Purpose

Module 1 vector of PlaMiNGo toolkit for expression of plastid targeted GFP1-10 in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

Chloroplastic FNR transit peptide

Use

Binary

Tags

GFP1-10

Expression

Plant

Promoter

'long' CaMV35S

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI127

Plasmid

#126067

Purpose

Module 1 vector of PlaMiNGo toolkit for expression of mitochondria targeted GFP1-10 in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Binary

Tags

GFP1-10

Expression

Plant

Promoter

'long' CaMV35S

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI177

Plasmid

#126077

Purpose

Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP1-10 and expression in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

ccdB Cassette (BsaI)
GFP1-10

Use

Binary, module 1

Tags

linker (GTGGGGS)

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI129

Plasmid

#126210

Purpose

Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x7 and expression in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

ccdB Cassette (BsaI)
GFP11x7 (5 aa linker)

Use

Binary, module 1

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI161

Plasmid

#126211

Purpose

Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11 and expression in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

ccdB Cassette (BsaI)
GFP11

Use

Binary, module 1

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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