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Plasmid#73419PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3H5.DepositorInsertPromoter 3H5 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterP3H5 (orthogonal T7-lac variant)Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET23a-MmUBB(SnaBI)
Plasmid#69558PurposeIn vitro transcription/translation (T7)DepositorInsertUBB (Ubb Mouse)
UseTagsExpressionBacterialMutationThe two last codons (TATTAA; encoding the last ty…PromoterT7Available sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET23a-MmUBB(Y305P)
Plasmid#69557PurposeBacterial expression; in vitro transcription/translation (T7)DepositorInsertUBB (Ubb Mouse)
UseTagsExpressionBacterialMutationCodon TAT encoding tyrosine 305 was changed to CC…PromoterT7Available sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET151-hUCH37 (ISF2)
Plasmid#61930PurposeExpression of hUCH37 (ISF2) in E.coliDepositorInserthUCH37 (ISF1) (UCHL5 Human)
UseTags6x HISExpressionBacterialMutationPromoterT7Available sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30Xa-SBL1(160E)
Plasmid#66846PurposeExpresses soybean lipoxygenase-1 S160E mutant with N-term HistagDepositorUseTagsHis-tagExpressionBacterialMutationS160EPromoterT7lacAvailable sinceSept. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET11+PCNA3-CYP119
Plasmid#66136PurposeExpresses the wild type of CYP119 fused to Sulfolobus solfataricus PCNA3DepositorInsertCYP119 (cyp119 )
UseTagsSulfolobus solfataricus PCNA3ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA3 T180C
Plasmid#66181PurposeExpresses the T180C mutant of Sulfolobus solfataricus PCNA3DepositorInsertDNA polymerase sliding clamp 3
UseTagsHis6 tagExpressionBacterialMutationT180CPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA3 R112C
Plasmid#66180PurposeExpresses the R112C mutant of Sulfolobus solfataricus PCNA3DepositorInsertDNA polymerase sliding clamp 3
UseTagsHis6 tagExpressionBacterialMutationR112CPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA2 E105C
Plasmid#66176PurposeExpresses the E105C mutant of Sulfolobus solfataricus PCNA2DepositorInsertDNA polymerase sliding clamp 2
UseTagsHis6 tagExpressionBacterialMutationE105CPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (Cow 1-82)
Plasmid#62062PurposeExpression of Cow p53 (residues 1-82) in e. coliDepositorInsertp53
UseTagsHisExpressionBacterialMutationcontains TAD residues 1-82PromoterT7Available sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (1-73) (K24N)
Plasmid#62079PurposeExpression of human p53 (1-73) (K24N) in e. coliDepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationcontains TAD residues 1-73; K24NPromoterT7Available sinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (Rabbit 1-87)
Plasmid#62058PurposeExpression of Rabbit p53 (residues 1-87) in e. coliDepositorInsertp53
UseTagsHisExpressionBacterialMutationcontains TAD residues 1-87PromoterT7Available sinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28A-p53 (1-73) (PPP)
Plasmid#62067PurposeExpression of human p53 (1-73) (P12A, P13A, P27A) in e. coliDepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationContains TAD residues 1-73; P12A, P13A, P27APromoterT7Available sinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-ceRab2(1-214)
Plasmid#59550Purposemakes 6x Fusion of Full length ceRab2DepositorInsert1-214
UseTagsExpressionMutationPromoterAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-ceRab5(1-208)
Plasmid#59551Purposemakes 6x Fusion of Full length ceRab5DepositorInsert1-208
UseTags6x HisExpressionMutationPromoterAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-ceRab10(1-201)
Plasmid#59552Purposemakes 6x Fusion of Full length ceRab10DepositorInsert1-208
UseTags6x HisExpressionMutationPromoterAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-ceRab11(1-211)
Plasmid#59553Purposemakes 6x Fusion of Full length ceRab11DepositorInsert1-211
UseTags6x HisExpressionMutationPromoterAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-ceRab21(1-207)
Plasmid#59554Purposemakes 6x Fusion of Full length ceRab21DepositorInsert1-207
UseTags6x HisExpressionMutationPromoterAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-ceRab21(1-209)
Plasmid#59555Purposemakes 6x Fusion of Full length ceRab35DepositorInsert1-209
UseTags6x HisExpressionMutationPromoterAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-20b-M-Ntx-S1
Plasmid#58716PurposeExpresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1SDepositorInsertNotexin
UseTagsMethionineExpressionBacterialMutationChanged Asparagine 1 to SerinePromoterT7Available sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only