Addgene

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ZE37 Nlk1(K117M)-pCS2+

Plasmid

#17078

Depositor

Randall Moon

Article

Thorpe et al Development. 2004 Jun;131(12):2899-909.

Insert

Nlk1 (nlk1 Zebrafish)

Use

Zebrafish expression

Tags

Expression

Mutation

K117M. Kinase dead mutant of NLK1.

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pAS-1 (perf)

Plasmid

#40836

Purpose

Target RNAs used to determine complementarity requirements for in vitro tailing and degradation

Depositor

Phillip Zamore

Article

Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

Insert

let-7–perf

Use

Mirna reporter

Tags

EGFP

Expression

Insect

Mutation

Promoter

Actin5C

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
RCAS(A) cTbx5 EnR (CT#635)

Plasmid

#13967

Depositor

Cliff Tabin

Article

Rallis et al Development. 2003 Jun . 130(12):2741-51.

Insert

Tbx5 (TBX5 Chicken)

Use

Retroviral; Avian expression

Tags

Expression

Mutation

Amino acids 1-274

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pLJD vector

Plasmid

#34595

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
ZE36 Nlk1(C387Y)-pCS2+

Plasmid

#17077

Depositor

Randall Moon

Article

Thorpe et al Development. 2004 Jun;131(12):2899-909.

Insert

Nlk1 (nlk1 Zebrafish)

Use

Zebrafish expression

Tags

Expression

Mutation

C387Y. Inactive mutant of NLK1.

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pGL2 enhancer- F4 LUC

Plasmid

#22434

Depositor

William Sessa

Article

Zhang et al J Biol Chem. 1995 Jun 23. 270(25):15320-

Insert

F4 (LOC110973015 Human)

Use

Luciferase

Tags

luciferase reporter gene

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2010

Availability

Academic Institutions and Nonprofits only
pGL2 enhancer- F5 LUC

Plasmid

#22436

Depositor

William Sessa

Article

Zhang et al J Biol Chem. 1995 Jun 23. 270(25):15320-

Insert

F5 (LOC110973015 Human)

Use

Luciferase

Tags

luciferase reporter gene

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2010

Availability

Academic Institutions and Nonprofits only
pJRL2 PHO5(UASm1)pr VYFP (EB#1549)

Plasmid

#14841

Depositor

Erin O'Shea

Article

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

PHO5 promoter UASm1 (PHO5 Budding Yeast)

Use

Tags

VYFP

Expression

Yeast

Mutation

UASm1 (AAGCTT was substituted for CACGTT). Unindu…

Promoter

Available since

May 25, 2007

Availability

Academic Institutions and Nonprofits only
pJRL2 PHO5(TATA-C2)pr ECFP (EB#1562)

Plasmid

#14844

Depositor

Erin O'Shea

Article

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

PHO5 promoter TATA-C2 (PHO5 Budding Yeast)

Use

Tags

ECFP

Expression

Yeast

Mutation

TATA-C2. TATA box mutated from TATATAAG to TCTA…

Promoter

Available since

May 25, 2007

Availability

Academic Institutions and Nonprofits only
RCAS(A) cTbx5 VP16 (CT#630)

Plasmid

#13969

Depositor

Cliff Tabin

Article

Rallis et al Development. 2003 Jun . 130(12):2741-51.

Insert

Tbx5 (TBX5 Chicken)

Use

Retroviral; Avian expression

Tags

Expression

Mutation

Amino acids 1-274

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
RFP->M71-IRES-M71-IRES-tauGFP ACNF TV

Plasmid

#15629

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):833-46.

Insert

RFP->M71-IRES-M71-IRES-tauGFP ACNF TV

Use

High copy number

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pQA-LP574

Plasmid

#222953

Purpose

Expresses R120A LoaP

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Use

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Arginine 120 to Alanine

Promoter

T7

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEC2483 (pSpy1C)

Plasmid

#220248

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQA-LP578

Plasmid

#222957

Purpose

Expresses R144S LoaP

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Use

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Arginine 144 to Serine

Promoter

T7

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQA-LP577

Plasmid

#222956

Purpose

Expresses K142S LoaP

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Use

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Lysine 142 to Serine

Promoter

T7

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pQA-LP576

Plasmid

#222955

Purpose

Expresses R141S LoaP

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Use

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Arginine 141 to Serine

Promoter

T7

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pQA-LP575

Plasmid

#222954

Purpose

Expresses R36S LoaP

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Use

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Arginine 36 to Serine

Promoter

T7

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pQA-LP573

Plasmid

#222952

Purpose

Expresses E40H LoaP

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Use

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Glutamate 40 to Histidine

Promoter

T7

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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MAC3_C_VPS35L

Plasmid

#209698

Purpose

Expresses MAC3-tagged VPS35L in mammalian cells

Depositor

Markku Varjosalo

Article

Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

Insert

VPS35L (VPS35L Human)

Use

Tags

MAC3-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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MAC_N_VPS26C

Plasmid

#209689

Purpose

Expresses MAC-tagged VPS26C in mammalian cells

Depositor

Markku Varjosalo

Article

Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

Insert

VPS26C (VPS26C Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge