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Plasmid#91085PurposeModule C, Promoter: At7SL, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, (+ BaeI site for GT donor cloning), Terminator: Pol IIIDepositorInsertEsp3I ccdb cassette for single gRNA spacer cloning
UseCRISPRTagsExpressionMutationPromoterAt7SLAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-EKAR
Plasmid#181858PurposeGenetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-EKAR
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pORANGE Cacnb4-GFP KI
Plasmid#139663PurposeEndogenous tagging of CaVβ4: C-terminal (amino acid position: H417)DepositorInsertgRNA and GFP donor
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pFETCh_FOXM1_iso1
Plasmid#135734PurposeDonor vector for 3' FLAG tag of human FOXM1_iso1DepositorInsertFOXM1_iso1 Homology arms (FOXM1 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pFETCh_GABPB1_iso2
Plasmid#104035PurposeDonor vector for 3' FLAG tag of human GABPB1_iso2DepositorInsertGABPB1_iso2 homology arms (GABPB1 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ARID4B
Plasmid#101072PurposeDonor vector for 3' FLAG tag of human ARID4BDepositorInsertARID4B homology arms (ARID4B Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Cacnb1 KI
Plasmid#139660PurposeEndogenous tagging of CaVβ1: N-terminal (amino acid position: S8)DepositorInsertgRNA and GFP donor
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_RERE
Plasmid#101069PurposeDonor vector for 3' FLAG tag of human REREDepositorInsertRERE homology arms (RERE Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-7xTcf-NLS-YNL
Plasmid#65711PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-YNL)DepositorInsert7xTcf, minCMV, 3xNLS, YNL
UseLuciferase; Tol2 systemTagsExpressionMutationPromoterAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
AI-CN590
Plasmid#62596PurposeKCTD13 N-terminus disruption by homolgy-directed repairDepositorInsertCerulean-P2A-PuroR (KCTD13 Human)
UseTALEN; Homology-directed repair donorTagsExpressionMammalianMutationPromoterhEF1alphaAvailable sinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
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PB-LacI-eGFP
Plasmid#213021PurposepiggyBac donor vector containing LacI-eGFP for random integration by PBaseDepositorInsertLacI**-eGFP
UseMouse Targeting; Piggybase integration (transposo…TagseGFPExpressionMammalianMutationPromoterAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hPER1-Luciferase-CD4-bla
Plasmid#189981PurposeDonor Vector to integrate firefly Luciferase C-terminal into the human PER1 Locus to express it as a fusion proteinDepositorInsertLuciferase
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hPER1-Halo-CD4-bla
Plasmid#189982PurposeDonor Vector to integrate HaloTag C-terminal into the human PER1 Locus to express it as a fusion proteinDepositorInsertHaloTag
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hCRY2-mScarlet-I-CD4-bla
Plasmid#189984PurposeDonor Vector to integrate mScarlet-I C-terminal into the human CRY2 Locus to express it as a fusion proteinDepositorInsertmScarlet-I
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hCRY2-Luciferase-CD4-bla
Plasmid#189985PurposeDonor Vector to integrate firefly Luciferase C-terminal into the human CRY2 Locus to express it as a fusion proteinDepositorInsertLuciferase
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only