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Plasmid#20346DepositorTypeEmpty backboneUseMouse Targeting; Gateway cloningTagsExpressionMutationPromoterAvailable sinceMarch 11, 2009AvailabilityAcademic Institutions and Nonprofits only
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pGGW_SM_BamHI
Plasmid#49315PurposeSubcloning vector for Golden Gate-based site-directed mutagenesis; BamHI and KpnI to release the insertDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMBPcyto-TEV-BseRI
Plasmid#157739Purposeempty vector adds TEV-protease-removable N-term His10 + maltose binding protein (without signal sequence); avoids unwanted residues in the final protein sequence by using the BseRI restriction sitesDepositorTypeEmpty backboneUseTagsHis10 + MBP (cytoplasmic expression; no signal se…ExpressionBacterialMutationPromoterT7Available sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pD(Gib)_3'omega
Plasmid#223870Purpose4G-cloning donor for 3'-Gibson overhang, omega-sequenceDepositorInsert3'omega sequence and spacer
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-TEV
Plasmid#223872Purpose4G-cloning donor for N-terminal His6-TEV tagDepositorInsertHis6-TEV
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-3C
Plasmid#223873Purpose4G-cloning donor for N-terminal His6-3C tagDepositorInsertHis6-3C
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-FLAG3
Plasmid#223874Purpose4G-cloning donor for N-terminal His6-FLAG3 tagDepositorInsertHis6-FLAG3
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(P+T)_ph
Plasmid#223857Purpose4G-cloning donor for insect promoter and terminator, no tag addedDepositorInsertpolyhedrin promoter and SV40 polyA
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(P+T)_CMV
Plasmid#223860Purpose4G-cloning donor for mammalian promoter and terminator, no tag addedDepositorInsertCMV promoter and bGH polyA
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(P+T)_T7
Plasmid#223854Purpose4G-cloning donor for bacterial promoter and terminator, no tag addedDepositorInsertT7 promoter and terminator
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-SUMO
Plasmid#223879Purpose4G-cloning donor for N-terminal His6-SUMO tagDepositorInsertHis6-SUMO
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_GFP-TEV
Plasmid#223881Purpose4G-cloning donor for N-terminal GFP-TEV tagDepositorInsertGFP-TEV
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_TS-3C
Plasmid#223883Purpose4G-cloning donor for N-terminal TwinStrep-3C tagDepositorInsertTwinStrep-3C
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_GFP-3C
Plasmid#223882Purpose4G-cloning donor for N-terminal GFP-3C tagDepositorInsertGFP-3C
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Ct)_3C-GFP
Plasmid#223888Purpose4G-cloning donor for C-terminal 3C-GFP tagDepositorInsert3C-GFP
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Ct)_3C-His8
Plasmid#223887Purpose4G-cloning donor for C-terminal 3C-His8 tagDepositorInsert3C-His8
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Ct)_CPD-His10
Plasmid#223890Purpose4G-cloning donor for C-terminal CPD-His10 tagDepositorInsertCPD-His10
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Ct)_3C-TS
Plasmid#223892Purpose4G-cloning donor for C-terminal 3C-TwinStrep tagDepositorInsert3C-TwinStrep
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Gib)_5'alpha
Plasmid#223863Purpose4G-cloning donor for 5'-Gibson overhang, alpha-sequenceDepositorInsert5'alpha sequence and spacer
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Gib)_5'gamma
Plasmid#223865Purpose4G-cloning donor for 5'-Gibson overhang, gamma-sequenceDepositorInsert5'gamma sequence and spacer
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only