Addgene

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Showing: 2461 - 2480 of 25717 results

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pMT1121

Plasmid

#8620

Depositor

Robert Hartley

Article

Hartley et al Protein Peptide Letters 1996 3:225-231

Insert

ribonuclease Sa, barstar

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only

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pSR1154

Plasmid

#221653

Purpose

Tetracycline sensor v3 for Staphylococcus aureus

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Inserts

sfGFP cassette
TetR cassette

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PXyl-TetO and PZwf

Available since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pVG329

Plasmid

#227771

Purpose

For genomic replacement of YOR1 gene in budding yeast

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al bioRxiv 2024.02.28.582517

Insert

Genomic sequences flanking YOR1 in budding yeast (YOR1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

N/A

Promoter

Available since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAM253

Plasmid

#226897

Purpose

mEOS3.2-PSP-GSS-FLAG-6xHis

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

mEOS3.2

Use

Tags

Prescission GSS-FLAG-6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM258

Plasmid

#226902

Purpose

6xHis-PSP-MBP

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

Maltose Binding Protein

Use

Tags

6xHis-PSP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pHC_O2

Plasmid

#205224

Purpose

part of Bactoneuron DF2, expresses mvenus

Depositor

Sangram Bagh

Article

Srivastava et al ACS Synth Biol. 2023 Jan 20;12(1):51-60.

Insert

mVenus

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHTC_O3

Plasmid

#205225

Purpose

part of Bactoneuron DF4, expresses mTagBFP2

Depositor

Sangram Bagh

Article

Srivastava et al ACS Synth Biol. 2023 Jan 20;12(1):51-60.

Insert

mTagBFP2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAC_O3

Plasmid

#205226

Purpose

part of Bactoneuron DF5, expresses mTagBFP2 and LuxR

Depositor

Sangram Bagh

Article

Srivastava et al ACS Synth Biol. 2023 Jan 20;12(1):51-60.

Insert

mTagBFP2, LuxR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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Lasso 1

Plasmid

#69935

Purpose

The first of the two RNA lassos required for the RNA chain construction

Depositor

Alfonso Jaramillo

Article

Hassall et al Methods Mol Biol. 2015;1316:149-67. doi:

Insert

Lasso 1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

TAATACGACTCACTATAGGG

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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Lasso 2

Plasmid

#69936

Purpose

The second of the two RNA lassos required for the RNA chain construction.

Depositor

Alfonso Jaramillo

Article

Hassall et al Methods Mol Biol. 2015;1316:149-67. doi:

Insert

Lasso 2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

TAATACGACTCACTATAGGG

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pLIC.B4.BSU37660

Plasmid

#11535

Depositor

Sung-Hou Kim

Article

Xu et al J Struct Funct Genomics. 2005 Nov 9. ():

Insert

phosphotransacetylase

Use

Tags

HisTag-MBP, TEV cleavable

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pNGFP-BC

Plasmid

#161956

Purpose

Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).

Depositor

Eric Gouaux

Article

Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1

Type

Empty backbone

Use

Tags

GFPuv His8

Expression

Bacterial

Mutation

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

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pCGFP-BC

Plasmid

#161959

Purpose

Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).

Depositor

Eric Gouaux

Article

Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1

Type

Empty backbone

Use

Tags

GFPuv His8

Expression

Bacterial

Mutation

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

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pNGFP-EU

Plasmid

#161967

Purpose

Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).

Depositor

Eric Gouaux

Article

Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1

Type

Empty backbone

Use

Tags

EGFP His8

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Availability

Academic Institutions and Nonprofits only

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pCGFP-EU

Plasmid

#161968

Purpose

Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).

Depositor

Eric Gouaux

Article

Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1

Type

Empty backbone

Use

Tags

EGFP His8

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Availability

Academic Institutions and Nonprofits only

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PG13-mCherry

Plasmid

#195291

Purpose

mCherry reporter containing 13 copies of the p53-binding consensus sequence

Depositor

Hideyuki Oguro

Article

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTn Donor

Plasmid

#236211

Purpose

Arabinose-inducible Tn5 InducTn-seq plasmid

Depositor

Matthew Waldor

Article

Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

Insert

None

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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GFP- RPL26

Plasmid

#31980

Depositor

Michael Kastan

Article

Takagi et al Cell. 2005 Oct 7. 123(1):49-63.

Insert

RPL26 (RPL26 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pSBtet-Pur

Plasmid

#60507

Purpose

SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and puromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Tags

Expression

Mammalian

Mutation

Promoter

TCE & RPBSA

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCre

Plasmid

#236212

Purpose

Arabinose-inducible Cre recombinase

Depositor

Matthew Waldor

Article

Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

Insert

Cre recombinase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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