Addgene

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pLV-tetO-Sox2

Plasmid

#19765

Depositor

Konrad Hochedlinger

Article

Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

Insert

Sox2 (Sox2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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YIp LYS2-HIS3

Plasmid

#122908

Purpose

LYS2-HIS3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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UAST-LbCas12a

Plasmid

#140621

Purpose

Gal4 dependent expression of LbCas12a in Drosophila

Depositor

Michael Boutros

Article

Port et al Proc Natl Acad Sci U S A. 2020 Aug 25. p

Insert

LbCas12a

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

UAST

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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p15A-OptoCre-cat-T172A-P-R

Plasmid

#189854

Purpose

Cre-inducible activation of cat-T172A for chloramphenicol resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Insert

P-loxP-TT-loxP-R-catT172A

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

P (T7 phage gene 10)

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pICE HIS3

Plasmid

#122877

Purpose

HIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a-EthR

Plasmid

#51170

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

EthR

Use

Synthetic Biology

Tags

6x-his

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pLZ12Km2-P23R:TA:Xen5

Plasmid

#88902

Purpose

Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

Depositor

Thomas Proft

Article

Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

Insert

toxin-antitoxin + LuxABCDE

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only
pCHNFI-X2

Plasmid

#31402

Depositor

Richard Gronostajski

Article

Chaudhry et al J Biol Chem. 1998 Jul 17. 273(29):18538-

Insert

Nuclear Factor IX isoform 2 (Nfix Mouse)

Use

Tags

NA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pICE TRP1

Plasmid

#122881

Purpose

TRP1 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pICE LYS2

Plasmid

#122879

Purpose

LYS2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 25, 2019

Availability

Academic Institutions and Nonprofits only

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pICE LEU2

Plasmid

#122878

Purpose

LEU2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCHNFI-C2

Plasmid

#31403

Depositor

Richard Gronostajski

Article

Chaudhry et al J Biol Chem. 1998 Jul 17. 273(29):18538-

Insert

Nuclear Factor IC isoform 2 (Ctf2 Mouse)

Use

Tags

NA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pECO200

Plasmid

#169765

Purpose

Express SpCas9 and Basta resistance gene

Depositor

Sang-Gyu Kim

Article

Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

Type

Empty backbone

Use

Plant binary vector

Tags

Expression

Plant

Mutation

Promoter

35S, AtU6

Available since

July 16, 2021

Availability

Academic Institutions and Nonprofits only

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pHM6g.AQ_1489

Plasmid

#11539

Depositor

Sung-Hou Kim

Article

Liu et al Proteins. 2003 Nov 1. 53(2):326-8.

Insert

tRNA methyltransferase

Use

Tags

HisTag-MBP, TEV cleavable

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
p15A-OptoCre-tetA-Ptet-Rtet

Plasmid

#189855

Purpose

Cre-inducible activation of tetA for tetracycline resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Insert

Ptet-loxP-TT-loxP-Rtet-tetA

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAEM7

Plasmid

#136422

Purpose

salL gene from S. tropica CNB-440 ligated into pHIS8

Depositor

Bradley Moore

Article

Eustaquio et al Nat Chem Biol. 2008 Jan;4(1):69-74. doi:

Insert

salL

Use

Tags

His8 tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pJW1667

Plasmid

#112041

Purpose

CEN/ARS plasmid for GAL-inducible ERG5-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

ERG5

Use

Tags

V5-TEV-EGFP

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pNR160

Plasmid

#79542

Purpose

Output plasmid: contains 5-state register made up of TP901 and BxbI recognition sites.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pDSAP

Plasmid

#62293

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, Puromycin resistance marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-HIS3

Plasmid

#122912

Purpose

TRP1-HIS3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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