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Plasmid#86896Purposeexpress E228A variant PvuRts1I in ER2566 strainDepositorInsertPvuRts1I (pvuRts1I )
UseTagsHisExpressionBacterialMutationE228APromoterT7Available sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-W215A-PvuRts1I
Plasmid#86898Purposeexpress W215A variant PvuRts1I in ER2566 strainDepositorInsertPvuRts1I (pvuRts1I )
UseTagsHisExpressionBacterialMutationW215APromoterT7Available sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-E71A-PvuRts1I
Plasmid#86895Purposeexpress E71A variant PvuRts1I in ER2566 strainDepositorInsertPvuRts1I (pvuRts1I )
UseTagsHisExpressionBacterialMutationE71APromoterT7Available sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-D57A-PvuRts1I
Plasmid#86892Purposeexpress D57A variant PvuRts1I in ER2566 strainDepositorInsertPvuRts1I (pvuRts1I )
UseTagsHisExpressionBacterialMutationD57APromoterT7Available sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-R208A-PvuRts1I
Plasmid#86891Purposeexpress R208A variant PvuRts1I in ER2566 strainDepositorInsertPvuRts1I (pvuRts1I )
UseTagsHisExpressionBacterialMutationR208APromoterT7Available sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-E20A-PvuRts1I
Plasmid#86897Purposeexpress E20A variant PvuRts1I in ER2566 strainDepositorInsertPvurts1I (pvuRts1I )
UseTagsHisExpressionBacterialMutationE20APromoterT7Available sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-D70A-PvuRts1I
Plasmid#86890Purposeexpress D70A variant PvuRts1I in ER2566 strainDepositorInsertPvuRts1I (pvuRts1I )
UseTagsHisExpressionBacterialMutationD70APromoterT7Available sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
csd2_121-308/pET21a(+)
Plasmid#83293Purposecsd2, construct: 121-308, pET21a(+) no-tagDepositorInserthp1544
UseTagsExpressionBacterialMutationdeleted amino acid 1-120PromoterAvailable sinceNov. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd5_41-192/pET28b(+)
Plasmid#83296Purposecsd5, construct: 41-192, pET28b(+) N-tagDepositorInserthp1250
UseTagsHis tagExpressionBacterialMutationdeleted amino acid 1-40PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd4_22-438/pET28b(+)
Plasmid#83295Purposecsd4, construct: 22-438, pET28b(+) N-tagDepositorInserthp1075
UseTagsHis tagExpressionBacterialMutationdeleted amino acid 1-21PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd2_140-251/pET21a(+)
Plasmid#83294Purposecsd2, construct:140-251, pET21a(+) C-tagDepositorInserthp1544
UseTagsHis tagExpressionBacterialMutationdeleted amino acids 1-139 and 252-308PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd1_54-312/pET21a(+)
Plasmid#83291Purposecsd1, construct: 54-312, pET21a(+) C-tagDepositorInserthp1543
UseTagsHis tagExpressionBacterialMutationdeleted amino acid 1-53PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd2_121-308/pET28b(+)
Plasmid#83292Purposecsd2, construct: 121-308, pET28b(+) NC-tagDepositorInserthp1544
UseTagsHis tagExpressionBacterialMutationdeleted amino acid 1-120PromoterAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd1_125-312/pET28b(+)
Plasmid#83290Purposecsd1, construct: 125-312, pET28b(+) NC-tagDepositorInserthp1543
UseTagsHis tagExpressionBacterialMutationdeleted amino acid 1-124PromoterAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-msGC-NT2
Plasmid#74763Purposeexpresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and betaDepositorInsertsfragment of alfa subunit of sGC
fragment of beta subunit of sGC
UseTagsHis-tagExpressionBacterialMutationfragment 49-471 of the alfa subunitPromoterT7Available sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK044 (pET-PurQ-wt)
Plasmid#73839PurposeProduces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, (PurQ)DepositorInsertformylglycinamidine ribonucleotide synthetase, glutaminase subunit
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM6-1D4-mCherry
Plasmid#73423PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1D4.DepositorInsertPromoter 1D4 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterP1D4 (orthogonal T7-lac variant)Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM6-4A6-mCherry
Plasmid#73424PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4A6.DepositorInsertPromoter 4A6 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterP4A6 (orthogonal T7-lac variant)Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM6-1E4-mCherry
Plasmid#73426PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1E4.DepositorInsertPromoter 1E4 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterP1E4 (orthogonal T7-lac variant)Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM6-1B6-mCherry
Plasmid#73420PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1B6.DepositorInsertPromoter 1B6 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterP1B6 (orthogonal T7-lac variant)Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only