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Plasmid#214068PurposeFluorescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTRDepositorInsertTurboRFP
UseTagsExpressionMammalianMutationLinker sequence present between RFP and GFPPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiRT
Plasmid#214067PurposeFluorescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTRDepositorInsertTurboRFP
UseTagsExpressionMammalianMutationLinker sequence present between RFP and GFPPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiRT_RTctrl
Plasmid#214070PurposeFluorescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTRDepositorInsertTurboRFP
UseTagsExpressionMammalianMutationLinker sequence present between RFP and GFPPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGG>nls-hCas9-nls
Plasmid#99138PurposeExpresses Cas9 via the chicken beta actin promoter for electroporation in chicken embryosDepositorInserthumanized Cas9
UseCRISPRTagsExpressionMutationPromoterChicken beta actin (CAG)Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
HH-gRNA-HDV-Shuttle-Vector
Plasmid#169098PurposeBackbone plasmid to generate HH-gRNA-HDV segmentDepositorInsertHH-gRNAbackbone-HDV
UseTagsExpressionMammalianMutationPromoterNAAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSCrhaB2plus
Plasmid#164226PurposeBroad-range bacterial expression vector with modified rhamnose-inducible promoter. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expression.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterRhamnose-inducibleAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
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T777T
Plasmid#113082PurposeEnhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence.DepositorTypeEmpty backboneUseRNAiTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-mouse-Caspase-1
Plasmid#183361PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-1 (Casp1 Mouse)
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAH25-SceI
Plasmid#129389PurposeDerived from pDAI-SceI. Tetracycline resistance removed and replaced with chloramphenicol resistance cassette from pKD3.DepositorInsertChloramphenicol acetyltransferase
UseTagsExpressionBacterialMutationN/APromoterCAT promoterAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs-OLIG2
Plasmid#32933DepositorInsertOLIG2 (OLIG2 Human)
UseRetroviralTagsExpressionMammalianMutationPromoter5'LTRAvailable sinceNov. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
ART_RTctrl
Plasmid#214066PurposeFluorescent reporter system, readthrough control, NMD negative control. No intron in 5'UTRDepositorInsertTurboRFP
UseTagsExpressionMammalianMutationLinker sequence present between RFP and GFPPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRBAD-Z-CGFP
Plasmid#52735PurposeL-(+)-arabinose-inducible expression of Z-CGFP positive control for in vivo split GFP assembly assayDepositorInsertZ+CGFP
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMay 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU-GFP-VHL
Plasmid#21053DepositorInsertvon Hippel-Lindau tumor suppressor (VHL Human)
UseYeast/bacteria shuttleTagsGFPExpressionYeastMutationPromoterAvailable sinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET11a-Z-NGFP
Plasmid#52734PurposeIPTG-inducible expression of Z-NGFP positive control for in vivo split GFP assembly assayDepositorInsertNGFP+Z-fusion
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:SEP-GluA1 TKIT donor
Plasmid#169418PurposeDonor DNA for TKIT knockin of SEP to GluA1 in mouseDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHFS:SEP-rGluA1 TKIT donor
Plasmid#169438PurposeDonor DNA for TKIT knockin of SEP to GluA1 in ratDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
miR-146a
Plasmid#15092DepositorInsertpre-miR-146a
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-Cherry-N1-PRKAG1
Plasmid#30308DepositorInsertAMP-activated Protein Kinase γ1 (PRKAA1 Human)
UseTagsFLAG and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDD284
Plasmid#66825PurposeTagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertTagRFP-T-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized TagRFP-TExpressionWormMutationPromoterAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only