Addgene

We narrowed to 3,126 results for: zebrafish

Showing: 2441 - 2460 of 3126 results

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Control-cx43.4-48H-pGTag

Plasmid

#117818

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

CAAX

Expression

Mutation

Promoter

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pQFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

Plasmid

#180474

Purpose

Inducible gene expression vector pQFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

Depositor

Hyunju Ro

Article

Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

Insert

QFDBD-2xQFAD*-VP16*-EcR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pErCas12a EZ Clone

Plasmid

#132641

Purpose

Expresses ErCas12a and an sgRNA to target a region of interest

Depositor

Stephen Ekker

Article

Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

Insert

ErCas12a

Use

AAV and CRISPR

Tags

3x HA and SV40 NLS

Expression

Mammalian

Mutation

Q926R (Please see depositor comments) and BsaI si…

Promoter

CMV Promoter

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mCerulean 156-239-GGGS-TDP-43 M337V

Plasmid

#162618

Purpose

Allows for transcription of mCerulean fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

M337V

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-GGGS-TDP43 M337V

Plasmid

#162612

Purpose

Allows for transcription of mVenus fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

M337V

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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EIF2B3

Plasmid

#172072

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

July 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mCerulean 156-239-GGGS-TDP-43

Plasmid

#162617

Purpose

Allows for transcription of mCerulean fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-I152L-GGGS-TDP-43

Plasmid

#162614

Purpose

Allows for transcription of improved mVenus I152L fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pPRISM-TagRFP-gcry-mRFP

Plasmid

#117766

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCSCRET2(#323)

Plasmid

#184058

Purpose

cyclofen-inducible CRE activation via mammalian cell transfection or mRNA synthesis

Depositor

Sophie Vriz

Article

Sinha et al Zebrafish. 2010 Jun;7(2):199-204. doi: 1

Insert

6myc-CRE-ERT2

Use

Synthetic Biology

Tags

6xMyc

Expression

Mammalian

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

sCMV+SP6

Available since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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pPRISM-TagRFP-gcry-eGFP

Plasmid

#117767

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPRISM-TagRFP-gcry-TagBFP

Plasmid

#117765

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPRISM-TagRFP-cmlc2-TagBFP

Plasmid

#117768

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2+ Myc-Kif26b

Plasmid

#102861

Purpose

Expresses mouse Kif26b in mammalian cells, zebrafish or Xenopus

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Mouse Kif26b (Kif26b Mouse)

Use

Zebrafish expression; xenopus expression

Tags

Expression

Mammalian

Mutation

Promoter

CMV; SP6

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pPRISM-Cre-gcry-eGFP

Plasmid

#117791

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

Cre

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-Fuse-cmlc2-TagBFP

Plasmid

#117786

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

mRFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2plus-mVenus1-155-GGGS-TDP-43

Plasmid

#162611

Purpose

Allows for transcription of mVenus fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2plus-mVenus1-155-I152L-GGGS-TDP43 M337V

Plasmid

#162615

Purpose

Allows for transcription of improved mVenus I152L fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

M337V

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPRISM-TagRFP-cmlc2-eGFP

Plasmid

#117770

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Control-cx43.4-48H-pPRISM

Plasmid

#117801

Purpose

Control to test technique on a target that is known to work

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge