Skip to main content
Addgene
Showing: 2441 - 2460 of 3154 results

  1. pOCC72

    Plasmid
    #118866
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEV
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease, mGFP, cleavable…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118866-plasmid-map-sequence-id-232323

    Enlarge

  2. pOCC28

    Plasmid
    #118873
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C--NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease and MBP, cleavab…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118873-plasmid-map-sequence-id-232344

    Enlarge

  3. SPDK3860

    Plasmid
    #199245
    Purpose
    Modified Tobacco rattle virus (TRV) RNA2 vector for editing of Nicotiana benthamiana Phytoene desaturase (PDS) gene
    Depositor
    Savithramma Dinesh-Kumar
    Article
    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41
    Insert
    Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
    Use
    T-dna vector
    Tags
    Expression
    Mutation
    Promoter
    pPEBV
    Available since
    June 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    199245-plasmid-map-sequence-id-396551

    Enlarge

  4. pOCC102

    Plasmid
    #118883
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C protease
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6-MBP, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118883-plasmid-map-sequence-id-232315

    Enlarge

  6. pRAN473

    Plasmid
    #120813
    Purpose
    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target protein
    Depositor
    David S. Weiss, Craig Ellermeier
    Article
    Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1
    Insert
    red fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Ptet
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120813-plasmid-map-sequence-id-235308

    Enlarge

  7. pDGE334

    Plasmid
    #153243
    Purpose
    M2E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
    Depositor
    Johannes Stuttmann
    Article
    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    153243-plasmid-map-sequence-id-305787

    Enlarge

  8. pYPQCas12j2

    Plasmid
    #189780
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLS, without promoter
    Depositor
    Yiping Qi
    Article
    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1
    Insert
    Cas12j2
    Use
    CRISPR; Gateway compatible cas12j2 entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    189780-plasmid-map-sequence-id-375101

    Enlarge

  9. pDGE331

    Plasmid
    #153240
    Purpose
    M1E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
    Depositor
    Johannes Stuttmann
    Article
    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    153240-plasmid-map-sequence-id-305768

    Enlarge

  10. pDGE337

    Plasmid
    #153246
    Purpose
    M4E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
    Depositor
    Johannes Stuttmann
    Article
    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    153246-plasmid-map-sequence-id-305804

    Enlarge

  11. pOCC167

    Plasmid
    #118909
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal HIS6, cleavable with 3C protease
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HBMss-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118909-plasmid-map-sequence-id-232266

    Enlarge

  12. pYPQ230-RV

    Plasmid
    #188544
    Purpose
    Gateway entry clone (attL1 & attR5) for LbCas12a-RV mediated genome editing
    Depositor
    Yiping Qi
    Article
    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:
    Insert
    LbCas12a-RV
    Use
    CRISPR; Gateway compatible lbcas12a-rv entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    G146R, R182V
    Promoter
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    188544-plasmid-map-sequence-id-369474

    Enlarge

  15. pOCC267

    Plasmid
    #118887
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal HALO tag
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-HALO-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6-MBP, cleavable with 3C protease; HALO tag
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118887-plasmid-map-sequence-id-232249

    Enlarge

  16. pHCas9-Nours

    Plasmid
    #107733
    Purpose
    Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae
    Depositor
    Sheng Yang
    Article
    Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.
    Insert
    Cas9
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Human optimized
    Promoter
    TEF1
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    107733-plasmid-map-sequence-id-208542

    Enlarge

  17. pDGE342

    Plasmid
    #153251
    Purpose
    M8E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
    Depositor
    Johannes Stuttmann
    Article
    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    153251-plasmid-map-sequence-id-305888

    Enlarge

  19. gCOTS-pyl

    Plasmid
    #92048
    Purpose
    This is a S. elongatus (PCC7942) shuttle vector used for recombination of the pylRS orthogonal translation system to genomic neutral site 2.
    Depositor
    Lital Alfonta
    Article
    Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/
    Inserts
    Pyrrolysyl tRNA(cua)
    Pyrrolysyl tRNA synthetase (methanosarcina mazei)
    Use
    Cyanobacteria s. elongatus genome recombination i…
    Tags
    Expression
    Mutation
    Promoter
    LeuP (native S. elongatus promoter) and PrcbL
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    92048-plasmid-map-sequence-id-181707

    Enlarge

Showing: 2441 - 2460 of 3154 results