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Plasmid#54040PurposeLocalization: Clathrin Vesicles, Excitation: 487, Emission: 509DepositorInsertClathrin (CLTB Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-Syn1-DIO-VGLUT2:miniSOG
Plasmid#214200PurposeFor Cre-dependent expression of VGLUT2:miniSOG (miniSOG is lumen-facing and HA-tagged)DepositorInsertSlc17a6 tagged with miniSOG and hemagglutinin
UseAAV and Cre/LoxTagsminiSOG (tagged with HA) is fused to the first in…ExpressionMammalianMutationPromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYNL-C1_Rab11a
Plasmid#65705PurposeExpresses YNL-Rab11a in mammalian cellsDepositorInsertRab11a (Rab11a Mouse)
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-ChrimsonR-tdTomato
Plasmid#171027PurposeExpresses ChrimsonR-tdTomato fusion after Flp-dependent recombinationDepositorHas ServiceAAV1 and AAV9InsertChrimsonR-tdTomato
UseAAVTagsChrimsonR is fused to tdTomatoExpressionMutationPromoterEf1aAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQLink-GST-10xCTD
Plasmid#138472PurposeExpresses GST-tagged 10xCTD heptapeptide repeat in bacteriaDepositorInsertRpb1 (POLR2A Human)
UseTagsGSTExpressionBacterialMutation10x CTD repeatPromoterT5Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPBC-LG3-tdT
Plasmid#62810PurposepPBC-LG3-tdT expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP3 and tdTomato under the control of the CAG promoter.DepositorInsertITR-CAG-Lck-GCaMP3-IRES-tdTomato-ITR
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP3-373
Plasmid#91779PurposeMammalian expression of low affinity calcium-sensing fluorescent proteinDepositorInsertGCaMP3-373
UseTags6xHis, T7 epitope, and Xpress tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOS003-lenti-CMV-GCaMPer
Plasmid#65227PurposeER calcium sensor for lentivirus productionDepositorInsertGCaMPer
UseLentiviralTagscircularly permuted GFPExpressionMammalianMutationGCaMP3 (D324G, D360G, D397G, D435G)PromoterCMVAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKT2/CAGXSP/PalmReNL
Plasmid#182970PurposeStably express PalmReNL in mammalian cells via the sleeping beauty transposon system.DepositorInsertPalmReNL
UseTagsExpressionMammalianMutationN/APromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAM-DCA-muGFP
Plasmid#166727PurposeAAV, encodes monomeric ultrastable GFP (muGFP)DepositorInsertmuGFP
UseAAVTagsExpressionMutationPromoterChicken Beta ActinAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMM643
Plasmid#112533PurposepZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanRDepositorInsertsHrtR
ChuA
luxCDABE
UseTagsExpressionBacterialMutationA17PPromoterJ23107, PL(HrtO), and ProDAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
canxTM-mEmerald (cytosolic)
Plasmid#186962PurposeExpresses mEmerald connected with the C-terminus of Calnexin transmembrane domain.DepositorInsertCANX (CANX Human)
UseTagsmEmeraldExpressionMammalianMutationOnly express calnexin (482-end) and add ER signal…PromoterCMV PromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCGN-ATF6 (1-373)m1
Plasmid#27174PurposeFor mammalian expression of the cytoplasmic domain of human ATF6 with mutations eliminating DNA binding and with an HA tag.DepositorInsertATF6 (ATF6 Human)
UseTagsHAExpressionMammalianMutationContains only aa 1-373 Amino acids 315–317 are c…PromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMT1-24X-NOG(AtUBI10)
Plasmid#202016PurposeT-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the AtUBI10 promoter.DepositorInsertsdCas9-24XGP41
NbGP41P-sfGFP-VP64-GB1
UseTagsGB1 and sfGFPExpressionPlantMutationPromoterAtUBI10Available sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPW3758 : CMVd3-HsIRE1-HaloTag
Plasmid#185680PurposeLow-level transient expression of full-length HsIRE1 with a C-terminal HaloTag.DepositorInsertERN1 (ERN1 Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SARS-CoV-2 Nsp3TMC111A
Plasmid#204207PurposeMammalian expression of SARS-CoV-2 Nsp3DepositorInsertSARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )
UseTagsFLAGx1ExpressionMammalianMutationCys to Ala in active site of PLproPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-GCaMP6f
Plasmid#128315PurposeExpresses GCaMP6f under control of eukaryotic Ef1a promoter after Flp-dependent recombination.DepositorHas ServiceAAV1InsertGCaMP6f
UseAAVTagsHis TagExpressionMammalianMutationPromoterEf1aAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
ATG-1929
Plasmid#108712PurposeCBR2opt in pF4Ag expression vector with T7 and CMV promoterDepositorInsertCBR2opt
UseTagsExpressionBacterial and MammalianMutationPromega used directed evolution to create CBR (Cl…PromoterT7, CMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti myc-NL-GW
Plasmid#113454PurposeLentiviral NanoLuc Gateway shuttle vector for N-terminal fusionsDepositorTypeEmpty backboneUseLentiviral and LuciferaseTagscmyc-NanoLucExpressionMammalianMutationPromoterhUbCAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GABRA1(A322D)-IRES2-EGFP
Plasmid#170821Purposehuman GABAA receptors alpha1 subunit carrying A322D mutation and IRES2-EGFPDepositorInsertHuman GABAA receptor alpha1 subunit carrying A322D mutation (GABRA1 Human)
UseTagsIRES2-EGFPExpressionMammalianMutationchange Alanine 322 to AspartatePromoterAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only