-
Plasmid#104589PurposeAAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse camk2aDepositorInsertcalcium/calmodulin-dependent protein kinase II alpha (Camk2a Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterNoneAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Actb KI #2
Plasmid#139666PurposeEndogenous tagging of β-actin: N-terminal (amino acid position: before startcodon)DepositorInsertgRNA and GFP donor
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSCL.180
Plasmid#185001PurposeExpress -Eco1 AAVS1 editing ncRNA and gRNADepositorInsertEco1: AAVS1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
UseTagsSV40NLSExpressionMammalianMutationAAVS1 donor, a1/a2 length extended for 27 bpPromoterH1Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.177
Plasmid#184998PurposeExpress -Eco1 EMX1 editing ncRNA and gRNADepositorInsertEco1: EMX1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
UseTagsSV40NLSExpressionMammalianMutationEMX1 donor, a1/a2 length extended for 27 bpPromoterH1Available sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pORANGE Bsn-GFP KI
Plasmid#139665PurposeEndogenous tagging of Bassoon: C-terminal (amino acid position: F3938)DepositorInsertgRNA and GFP donor
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHX_HDR_hPAX7_IRES-EGFP-PGK-Neo
Plasmid#160458PurposeHomology-directed recombination donor vector for EGFP reporter knock-in to human PAX7 3' UTRDepositorUseInsertion of a fluorescent reporter through homol…TagsExpressionMutationPromoterAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pORANGE unc13a GFP KI
Plasmid#131498PurposeEndogenous tagging of Munc13-1: C-terminal (amino acid position: A1762)DepositorInsertgRNA and GFP donor (Unc13a Rat)
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-mClover3-Puromycin
Plasmid#167205PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mClover3 and selection with PuromycinDepositorTypeEmpty backboneUseCRISPRTagsmClover3ExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
HR180-LGR5-CreER
Plasmid#129093PurposeKI CreER with a puromycin/mRuby selection cassette into human LGR5 locusDepositorInsertCreERT2 and LGR5 homology arms (LGR5 Human)
UseHomologous recombination donor plasmidTagsExpressionMutationPromoterAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pODN-AAVS1-mNG-CAAX
Plasmid#183870PurposeRepair template for the stable expression of a mNeonGreen-CAAX fusion in human cells using CRISPR/Cas9.DepositorInsertAAVS1 homology arms with CMV-driven mNeonGreen-CAAX fusion (AAVS1 Human)
UseCRISPR; Donor templateTagsmNeonGreen-CAAXExpressionMammalianMutationHomology arms contain point mutations to remove t…PromoterCMVAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFD5-U6-3-t-attP40
Plasmid#133561PurposegRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructsDepositorInsertattP40 region guide RNAs
UseCRISPRTagsExpressionInsectMutationPromoterU6Available sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
FMR1-E17C-3xFLAG
Plasmid#157784Purposedonor plasmid for C-terminal FLAG tag fusion of human FMR1DepositorInsertFMR1-FLAG (FMR1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-mTagBFP2-Blasticidin
Plasmid#167207PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with BlasticidinDepositorTypeEmpty backboneUseCRISPRTagsmTagBFP2ExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pODN-AAVS1-mR2-CAAX
Plasmid#183871PurposeRepair template for the stable expression of a mRuby2-CAAX fusion in human cells using CRISPR/Cas9.DepositorInsertAAVS1 homology arms with CMV-driven mRuby2-CAAX fusion (AAVS1 Human)
UseCRISPR; Donor templateTagsmRuby2-CAAXExpressionMammalianMutationHomology arms contain point mutations to remove t…PromoterCMVAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-hSOX10
Plasmid#24749DepositorInsert(sex determining region Y)-box 10 (SOX10 Human)
UseGateway donor vectorTagsExpressionMutationwildtype sequencePromoterAvailable sinceMay 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMS_375
Plasmid#182132Purposeexpresses retron RNA, ec.86 Reverse Transcriptase, CspRecT SSAP, and mutL* to create a specific rifampicin resistance mutation while inhibiting MMRDepositorInsertsretron RNA
ec.86 RT
CspRecT
UseTagsExpressionMutationincorporated rifampicin-resistance-conferring don…PromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-mRuby3-Zeo
Plasmid#167206PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mRuby3 and selection with ZeocinDepositorTypeEmpty backboneUseCRISPRTagsmRuby3ExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-RAB11a KI
Plasmid#131499PurposeEndogenous tagging of RAB11: N-terminal (amino acid position: before startcodon)DepositorUseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Bsn KI
Plasmid#139664PurposeEndogenous tagging of Bassoon: N-terminal (amino acid position: L7)DepositorInsertgRNA and GFP donor
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
PL652
Plasmid#159456PurposeDonor empty vector with puromycin resistance gene expression cassette flanked by FRTDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only