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Plasmid#154197PurposeLentiviral expression plasmid encoding two sgRNAs without a target. These can be used as a off-target control for CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.DepositorInsertsgRNA-BC2-A, sgRNA-BC2-C
UseLentiviral; Catch barcode activationTagsExpressionMammalianMutationPromoterhU6 and mU6Available sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
CaTCH Dual-sgRNA_sgBC1-A_sgBC1-C
Plasmid#154196PurposeLentiviral expression plasmid encoding two sgRNAs for targeting of the CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.DepositorInsertsgRNA-BC1-A, sgRNA-BC1-C
UseLentiviral; Catch barcode activationTagsExpressionMammalianMutationPromoterhU6 and mU6Available sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-ataxin-3 C-t
Plasmid#89981PurposeExpresses the GFP-tagged C-terminus of ataxin-3DepositorInsertataxin-3 (ATXN3 Human)
UseTagsGFPExpressionMammalianMutationdeleted amino acids 1-182PromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-pro-Il1b-C-Flag
Plasmid#75131PurposeExpresses C-terminal flag-tagged pro-IL-1β in mammalian cellsDepositorInsertinterleukin 1 beta (Il1b Mouse)
UseTags3XFLAGExpressionMammalianMutation(please see depositor comments below)PromoterCMVAvailable sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPHAGE-CR-PINK1-C-TAP
Plasmid#128510PurposeLentiviral constitutive expression of CRISPR-resistant PINK1 with c-terminal FLAG and HA tag.DepositorInsertPTEN induced kinase 1 (PINK1 Human)
UseLentiviralTagsFLAG and HA tagsExpressionMammalianMutationSynonymous silent mutation in Q5 to render sgRNA …PromoterCMVAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPHAGE-CR-NENF-C-TAP
Plasmid#128511PurposeLentiviral constitutive expression of CRISPR-resistant NENF with c-terminal FLAG and HA tag.DepositorInsertNENF (NENF Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationSynonymous silent mutations to F81, Y82, G83 and …PromoterCMVAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pclbw-opa1(isoform 7DeltaS1)-myc
Plasmid#62846PurposeMammalian Expression of myc-tagged Opa1 isoform 7 Delta S1DepositorInsertmmOPA1isoform7 DeltaS1 - myc (Opa1 Mouse)
UseRetroviralTags9xMycExpressionMammalianMutationPromotercmv-bactinAvailable sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO Myc ArA K162R
Plasmid#59805PurposeAllows the integration of Myc ArA K162R in the genome and Tet-inducible expressionDepositorInsertAurora A (AURKA Human)
UseTagsMycExpressionMammalianMutationK162R, kinase mutantPromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO Myc ArB WT RNAiR
Plasmid#59806PurposeAllows the integration of Myc ArB in the genome and Tet-inducible expression.DepositorInsertAurora B (AURKB Human)
UseTagsMycExpressionMammalianMutationRNAi resistantPromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 TO FRT Myc ArB K106R
Plasmid#59807PurposeAllows the integration of Myc ArB K106R in the genome and Tet-inducible expressionDepositorInsertAurora B (AURKB Human)
UseTagsMycExpressionMammalianMutationK106R, kinase mutantPromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV_Myc-CylM-P2A-FLAG-YFast-TEV-His-CylLL-S15T
Plasmid#225063PurposeExpression of CylM-modified CylLL-S15T in human cellsDepositorInsertCylLL-S15T
UseTagsMultiple tags due to polycistronic expressionExpressionMammalianMutationS15TPromoterCMVAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
(318) pcDNA3.1-myc-OGA(1-400)D174N
Plasmid#168096PurposeMyc tagged inactive truncated human OGA catalytic domain of residues 1-400 with D174N mutationDepositorInsertmyc-OGA(1-400, D174N)
UseTagsmycExpressionMammalianMutationD174N catalytically inactive OGAPromoterT7/CMVAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-Myc-BioID2-MCS1 (JDW 1095)
Plasmid#224510PurposeGateway compatible middle entry clone containing a Myc-tagged BIOID2 constructDepositorInsertMyc-BioID2
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-Myc-BioID2-MCS2 (JDW 1209)
Plasmid#224511PurposeA Gateway compatible middle clone with a Myc-tagged BIOID2 construct followed by a flexible linker and an MCSDepositorInsertMyc-BioID2
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUASp-Flag-Myc-Qin-Tudor-attB
Plasmid#44210DepositorInsertQin-Tudor-domain (qin Fly)
UseFly transformationTagsFlag and MycExpressionMutationlacks E3 domain on N-term (del aa1-274)PromoterAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
XE62 Xwnt-4 myc-SP64T
Plasmid#16877DepositorInsertXwnt-4 myc (wnt4 Frog)
UseXenopus expressionTagsC-MycExpressionMutationPromoterAvailable sinceMarch 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-GFP-TNRC6A-SD-S1332A/S1346A/S1616A/S1691A
Plasmid#215905PurposeExpression of TNRC6A-silencing domain (SD) with S1332A/S1346A/S1616A/S1691A mutationDepositorInsertTNRC6A (TNRC6A Human)
UseTagsmyc-GFPExpressionMammalianMutationcontain aa1224-1709, S1332A, S1346A, S1616A, S169…PromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-BirA-Linker-Myc-mRap1-I312R
Plasmid#184645PurposeRetroviral expression of BirA Rap1 I312RDepositorInsertBirA-rap1-I312R
UseRetroviralTagsBirA FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TurboID-3MYC-kanMX6 (pFB1420)
Plasmid#126049PurposeFor C-terminal tagging with TurboID in yeast including geneticin-resistant markerDepositorInsertTurboID
UsePcr-based c-terminal taggingTagsExpressionMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TurboID-3MYC-natMX6 (pFB1434)
Plasmid#126050PurposeFor C-terminal tagging with TurboID in yeast including nourseothricin-resistant markerDepositorInsertTurboID
UsePcr-based c-terminal taggingTagsExpressionMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only