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Showing: 2441 - 2460 of 30517 results

  4. pCMV-PEmax-P2A-hMLH1dn

    Plasmid
    #174828
    Purpose
    Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)
    Depositor
    David Liu
    Article
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Insert
    PEmax-P2A-hMLH1dn
    Use
    Tags
    SV40 bpNLS and c-Myc NLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript; deletion of residues 754-…
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174828-plasmid-map-sequence-id-357934

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  9. pHAGE-CMV-Flag-CDK12_IDG-K

    Plasmid
    #135281
    Purpose
    Gateway destination clone of CDK12 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics
    Depositor
    Ben Major
    Insert
    Flag-CDK12 (CDK12 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135281-plasmid-map-sequence-id-267208

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  14. pCMV-dCas13-inactive M3nls

    Plasmid
    #157854
    Purpose
    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant
    Depositor
    David Liu
    Article
    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103
    Insert
    dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    PspCas13b Δ984-1090 H133A; METTL3 D395A
    Promoter
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    157854-plasmid-map-sequence-id-307627

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Showing: 2441 - 2460 of 30517 results