-
Plasmid#118867Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mRuby3DepositorInsertNcoI-HIS6-3C-mRuby3-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease, mRuby3ExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC61
Plasmid#118877Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
UseTagsmGFP, HIS6, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166
Plasmid#109328PurposeGateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoterDepositorInsertzCas9
UseCRISPR; Gateway compatible zcas9 entry cloneTags3x FLAG, NLS and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXD70DA9-Pct5-DadhABC(A2)
Plasmid#191632PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strainDepositorInsertDopamine dehydroxylase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAD-SPS6-Pman
Plasmid#133906PurposeFluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.DepositorInsertsfGFP(SPS6)
UseE.coli/bacillus spp. shuttle vectorTagsExpressionBacterialMutationPromoterPmanAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS1ClacZ
Plasmid#55171PurposelacZ-reporter vector, integration at amyE, ampr, cmrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoternoneAvailable sinceAug. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYES1L-URA
Plasmid#84301PurposeE.coli shuttle vector for large-scale DNA assembly in S. cerevisiae.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_P0826-BB0323-mCherryBb
Plasmid#118244PurposeKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes a BB0323-mCherry translational fusion under the control of the bb0826 promoterDepositorInsertBB0323-mCherry translational fusion under the control of the bb0826 promoter
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS3Clux
Plasmid#55172Purposelux-reporter vector, integration at sacA, ampr, cmrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoternoneAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJAK184
Plasmid#167281PurposeMazF-based mutagenesis vector for C. difficileDepositorTypeEmpty backboneUseE. coli - c. difficile shuttle vectorTagsExpressionMutationPromoterPxylAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1
Plasmid#166823PurposeGateway pDONR vector containing NLRP1 (no stop codon)DepositorInsertNLRP1 (NLRP1 Human)
UseGateway shuttling vectorTagsExpressionMutationPromoterAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8
Plasmid#169962PurposepDONR221 for Gateway shuttling. Contains the CARD8 T60 isoform (no stop codon)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR E3 pR8A L3-L2
Plasmid#158735PurposePlant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last NN)DepositorInsertTALEN ORF without TAL repeat
UseTALENTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR E3 pR5A L3-L2
Plasmid#158732PurposePlant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last HD)DepositorInsertTALEN ORF without TAL repeat
UseTALENTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR E3 pR6A L3-L2
Plasmid#158733PurposePlant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last NG)DepositorInsertTALEN ORF without TAL repeat
UseTALENTagsExpressionMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR E3 pR7A L3-L2
Plasmid#158734PurposePlant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last NI)DepositorInsertTALEN ORF without TAL repeat
UseTALENTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAD-mKate(KPS12)
Plasmid#133831PurposeFluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides.DepositorInsertmKate(KPS12)
UseE.coli/bacillus spp. shuttle vectorTagsExpressionBacterialMutationPromoterPuppAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS4S
Plasmid#55170PurposeEmpty vector, integration at thrC, ampr, specrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoternoneAvailable sinceAug. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJAK080
Plasmid#167279PurposeCodA-based vector for insertion of DNA by recombination downstream of the pyrE gene in C. difficileDepositorTypeEmpty backboneUseE. coli - c. difficile shuttle vectorTagsExpressionMutationPromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pdCASclos
Plasmid#73640PurposeTranscriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824DepositorInsertsdCas9
sgRNA to spo0A
UseE.coli-clostridium shuttle vectorTagsExpressionMutationD10A, H840APromoterPj23119 and ptbAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only