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Plasmid#112944PurposeProduces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRETDepositorInsertmCherry and LSS-SGFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Top-Toehold-mCherry
Plasmid#87318PurposeExpresses the "Top" transcript of the Split-Broccoli system upstream of an RNA Toehold switch controlling mCherry.DepositorInsertSplit-Broccoli "Top" transcript upstream of an RNA Toehold (TS2_KS01) controlling mCherry translation.
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP70aAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
AausFP1-T2A-mCherry pdrf1-GW
Plasmid#168071PurposeProduces equimolar expression of AausFP1 and mCherry for use in yeastDepositorInsertAausFP1-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS305:Met3p-mCherry-Tub1
Plasmid#37553DepositorInsertMet3p-mCherry-Tub1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCherry-Emi1_control UTRs
Plasmid#67925PurposeExpresses mCherry-Emi1 with control UTRs in mammalian cellsDepositorInsertmCherry-Emi1 (FBXO5 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
KA1130_pPB-TREtight-cHA-IRESH2BmCherry
Plasmid#124173PurposepiggyBac-based Dox-inducible expressionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS1-rab-3P::his-24::mCherry
Plasmid#124348PurposeNuclear Localized Pan-Neuronal mCherryDepositorInsertrab-3, mCherry (rab-3 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
10XUAS IVS ChrimsonR mCherry_tr
Plasmid#111547PurposeOptogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherryDepositorInsertChrimsonR_mCherry_trafficked
UseTagsExpressionInsectMutationwtPromoterhsp70Available sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-SAC1C389S-HLx8
Plasmid#108138PurposeRecruitable inactive SAC1 with extended linkerDepositorInsertmCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)
UseTagsExpressionMammalianMutationSACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587)PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV BARK p2A mCherry
Plasmid#117689PurposeCMV-iBARK-p2A-mCherry: Mammalian expression vector for Gaq silencingDepositorInsertBARKrgs peptide
UseTagsHA / FLAGExpressionMammalianMutationPromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymTurquoise2-T2A-mCherry
Plasmid#118442PurposeProduces equimolar expression of yeast codon-optimized mTq2 and mCherryDepositorInsertymTurquoise2-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-3xNLS-OptoJNKi-dsm
Plasmid#89743PurposeExpression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11DepositorInsertNLS-OptoJNKi dark-state mutant
UseFluorescent proteinTagsmCherryExpressionMammalianMutationC450A mutation of AsLov2JalphaPromoterCMVAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-3xNLS-OptoJNKi-lsm
Plasmid#89742PurposeExpression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11DepositorInsertNLS-OptoJNKi lit-state mutant
UseFluorescent proteinTagsmCherryExpressionMammalianMutationI539E mutation of AsLov2JalphaPromoterCMVAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS306:Met3p-mCherry-Tub1
Plasmid#37554DepositorInsertMet3p-mCherry-Tub1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mCherry-CyrCR1.miR17
Plasmid#128748PurposeExpresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-17 site inserted in mCherry 3'UTR.DepositorInsertCyrano conserved region (CR1) with miR-17 site replacing miR-7 site (OIP5-AS1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cas9 ROSA sgRNA mCherry
Plasmid#155280PurposeLentiviral Cas9 expression, ROSA sgRNA expression, mCherry markerDepositorInsertspCas9
UseTagsExpressionMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCF820_U6-sgRNA-EF1a-mCherry2_lenti
Plasmid#138316PurposeLenti expression of mCherry2 with U6 promoter for SpyCas9 sgRNA targeting GFPDepositorInsertSpyCas9 GFP sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D-mCherry-hPMCA2x/b
Plasmid#111569PurposeExpresses a plasma membrane calcium pump hPMCA2x/bDepositorInsertPlasma membrane calcium pump 2, variant x/b (ATP2B2 Human)
UseTagsmCherryExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_CS M_tTA-BFP
Plasmid#58874PurposeMESA target chain with mCherry ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nAChr-Alpha6-mCherry
Plasmid#50480Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChr-alpha6 (Chrna6 Mouse)
UseTagsmCherry fusion in M3-M4 loop (after residue A405)ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only