Skip to main content
Addgene
Showing: 2421 - 2440 of 2442 results

  2. pTK Cas9-2A-Citrine

    Plasmid
    #92393
    Purpose
    Enhancer/reporter plasmid for tissue-specific expression of Cas9 with 2A-Citrine reporter. Contains BsmBI-flanked LacZ cloning cassette for rapid GoldenGate-based cloning of specific enhancers.
    Depositor
    Tatjana Sauka-Spengler
    Article
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Insert
    Cas9 2A Citrine
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    thymidine kinase promoter
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    92393-plasmid-map-sequence-id-190866

    Enlarge

  4. pUAP1

    Plasmid
    #63674
    Purpose
    Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.
    Depositor
    Nicola Patron
    Article
    Patron et al New Phytol. 2015 Jul 14. doi: 10.1111/np
    Insert
    RFP cloning selection cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    63674-plasmid-map-sequence-id-106088

    Enlarge

  5. Yamamoto Lab TALEN Accessory Pack

    Plasmid Kit
    #1000000030
    Purpose
    Contains modified pFUS array vectors and destination vectors that are designed for use with the Golden Gate TALEN and TAL Effector Kit.
    Depositor
    Takashi Yamamoto
    Available since
    March 28, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. Mobius Assembly Vector Toolkit

    Plasmid Kit
    #1000000134
    Purpose
    Mobius Assembly: Uses a two-level approach for transcriptional unit (TU) and multi-TU augmentation. Each level is comprised of four Acceptor Vectors. Compatible with Golden Gate parts
    Depositor
    Naomi Nakayama
    Available since
    March 13, 2018
    Availability
    Academic Institutions and Nonprofits only

  7. Multiplex CRISPR/Cas9 Assembly System Kit

    Plasmid Kit
    #1000000055
    Purpose
    Golden Gate cloning assembly for construction of all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs with either Cas9 nuclease or Cas9 D10A nickase; see kit #1000000062 for FokI Accessory Pack
    Depositor
    Takashi Yamamoto
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. Ralstonia-GG-Kit

    Plasmid Kit
    #1000000142
    Purpose
    Backbone vectors and Level I modules that can be used for assembly of Ralstonia solanacearum species complex gene knockout and complementation constructs based on Golden Gate cloning techniques
    Depositor
    Thomas Lahaye
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only

  9. pDGE Dicot Genome Editing Kit

    Plasmid Kit
    #1000000084
    Purpose
    Golden Gate cloning-based assembly of constructs for CRISPR genome editing in dicotyledonous plants. Consists of “genome editing recipient” and “sgRNA shuttle” vectors.
    Depositor
    Johannes Stuttmann
    Available since
    Sept. 9, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. Komagataeibacter Tool Kit (KTK)

    Plasmid Kit
    #1000000216
    Purpose
    The Komagataeibacter Tool Kit (KTK) is a standardized modular cloning system based on Golden Gate DNA assembly.
    Depositor
    Tom Ellis
    Available since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only

  11. Yeast Secrete and Detect

    Plasmid Kit
    #1000000166
    Purpose
    Yeast Golden Gate cloning for protein secretion in Saccharomyces cerevisiae and Pichia pastoris. Methanol utilization (MUT) promoters, signal peptides, C-terminal tags, episomal and integrative.
    Depositor
    Sylvestre Marillonnet
    Available since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  12. MoClo Plant Parts Kit

    Plasmid Kit
    #1000000047
    Purpose
    Modular Cloning (MoClo) collection contains 95 standardized parts to enable Golden Gate construction of multi-gene constructs for plant transformation
    Depositor
    Nicola Patron, Sylvestre Marillonnet
    Available since
    April 8, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. T2A Split/Link TurboID Kit

    Plasmid Kit
    #1000000249
    Purpose
    The T2A Split/Link TurboID Kit uses a sequential Golden Gate assembly method and can be used to assemble mammalian expression vectors for proximity labeling experiments.
    Depositor
    Sven Eyckerman
    Available since
    Aug. 7, 2024
    Availability
    Academic Institutions and Nonprofits only

  14. EcoFlex MoClo Kit

    Plasmid Kit
    #1000000080
    Purpose
    Golden Gate kit for E. coli synthetic biology; consists of a modular library that uses MoClo assembly standard for a variety of uses, e.g. recombinant protein purification, cell-free circuit design
    Depositor
    Paul Freemont
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  15. Fragmid

    Plasmid Kit
    #1000000237
    Purpose
    Fragmid is a modular toolkit that leverages Golden Gate cloning to enable the rapid creation of CRISPR vectors compatible with various delivery approaches, enzymes, and mechanisms.
    Depositor
    John Doench
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only

  16. EMMA toolkit

    Plasmid Kit
    #1000000119
    Purpose
    Used for the construction of a variety of mammalian expression vectors in a one-pot, one-step Golden Gate reaction; compatible with automated production
    Depositor
    Yizhi Cai
    Available since
    March 1, 2018
    Availability
    Academic Institutions and Nonprofits only

  17. MoClo Plant Parts III: Transformation & Genome Engineering Kit

    Plasmid Kit
    #1000000236
    Purpose
    The MoClo Plant Parts III: Transformation & Genome Engineering Kit contains 94 Level 0 parts, compatible with the Phytobrick/MoClo system for golden gate assembly of plant transformation constructs.
    Depositor
    Daniel Voytas
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  18. The Mammalian Toolkit

    Plasmid Kit
    #1000000180
    Purpose
    A ready-made extensive library of plasmids that enable Golden Gate high-throughput assembly of genetic circuits that can be delivered to cells in a multitude of ways.
    Depositor
    Hana El-Samad
    Available since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only

  19. MoChlo: Modular Cloning Chloroplast Toolbox

    Plasmid Kit
    #1000000156
    Purpose
    The MoChlo Toolbox consists of a library of standardized chloroplast-specific genetic modules for Golden-Gate cloning of synthetic operons into chloroplast transformation vectors.
    Depositor
    Scott Lenaghan
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only

  20. Cultivarium POSSUM Toolkit

    Plasmid Kit
    #1000000234
    Purpose
    The POSSUM Toolkit is a Golden Gate genetic toolkit developed to help researchers identify functional plasmids and establish genetic tractability in non-model microbes.
    Depositor
    Nili Ostrov
    Available since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 2421 - 2440 of 2442 results