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Plasmid#181857PurposeGenetically encoded FRET-based sensor for monitoring JNK kinase activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-JNKAR
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
T-2A-EGFP-PGK-Puro
Plasmid#83344PurposeDonor vector to knocin EGFP into human Brachyury(T) locusDepositorInsertHuman Brachyury knockin homology arms (TBXT Human)
UseTags2A-EGFPExpressionMutationPromoterAvailable sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pODN-mNG-RHOA
Plasmid#183836PurposeRepair template for the N-terminal tagging of RhoA with mNeonGreen in human cells using CRISPR/Cas9.DepositorInsertRHOA homology arms with mNeonGreen-linker (RHOA Human)
UseCRISPR; Donor templateTagsmNeonGreen-linkerExpressionMammalianMutationHomology arms contain point mutations to remove t…PromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hPER1-mScarlet-I-CD4-bla
Plasmid#189980PurposeDonor Vector to integrate mScarlet-I C-terminal into the human PER1 Locus to express it as a fusion proteinDepositorInsertmScarlet-I
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR-U6gRNA-PGKpuro2APDGFB
Plasmid#136409PurposeDonor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses PDGFB.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationBbsI restriction site in the PDGFB sequence (Glu1…PromoterU6, PDKAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDsRed-attP
Plasmid#51019PurposeVector for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. As known as pHD-DsRed-attPDepositorInsertsattP
LoxP-3xP3-DsRed-LoxP
UseHigh copy, amp resistanceTagsExpressionMutationPromoter3xP3 and NoneAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTN4
Plasmid#174212PurposeDonor plasmid for introducing Peft-3::AtTIR1(F79G)::mRuby at the ttTi5605 locusDepositorInsertsUseCRISPR and Cre/LoxTagsmRubyExpressionWormMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1_EF1a-GEM2-Halo-FKBP-IRES-Blast
Plasmid#197066PurposeDonor plasmid for human AAVS1 locus knock-in, constitutive expression of GEM2-Halo-FKBPDepositorInsertEncapsulin
UseCRISPRTagsFKBP and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCL.175
Plasmid#184996PurposeExpress -Eco1 HEK3 editing ncRNA and gRNADepositorInsertEco1: HEK3 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
UseTagsSV40NLSExpressionMammalianMutationHEK3 donor, a1/a2 length extended for 27 bpPromoterH1Available sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV ORANGE Dlg4-HaloTag KI
Plasmid#139656PurposeEndogenous tagging of PSD95: C-terminal (amino acid position: R721)DepositorInsertgRNA and HaloTag donor
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-HDR-mEGFP-Actin
Plasmid#119870PurposeAAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse Beta ActinDepositorInsertbeta Actin (Actb Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-HOT-Clover-BlastR
Plasmid#138569PurposeUniversal Cleavable Donor Plasmid for tagging with Clover-BlastR using NHEJDepositorInsertClover-BlastR
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
SOX9-T2A-H2B-EGFP repair template
Plasmid#167972PurposeSOX9 reporter repair templateDepositorInsertSRY-Box Transcription Factor 9 (SOX9 Human)
UseCRISPR; Donor templateTagsH2B-EGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Pclo KI
Plasmid#139657PurposeEndogenous tagging of Piccolo: N-terminal (amino acid position: G2)DepositorInsertgRNA and GFP donor
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBR_attB(bxb)_ccdB_lox
Plasmid#183763PurposeBxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)DepositorInsertccdB
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMIA10.53-Clover
Plasmid#214450PurposeBxbI landing-pad mClover-IRES-puro donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates constitutively expressing cell line.DepositorInsertmClover
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD/Paro-IFN
Plasmid#205439PurposeA donor vector plasmid for Cre-mediated integration of paromomycin resistance (Paro) and dog IFNα-4 (IFNα-4) genesDepositorInsertsdog interferon α-4 gene
paromomycin resistance gene
UseCre/Lox; Chlamydomonas expressionTagsExpressionMutationPromoterHeat shock protein 70A (HSP70A)/ribulose bisphosp…Available sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Oct4-IRES-eGFP-PGK-Neo
Plasmid#48681PurposeFor knock-in donor vector by CRISPR/CAS systemDepositorInsertOct4 (Pou5f1 Mouse)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Neo-CAG-Flpe-ERT2
Plasmid#68460PurposeAAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and Neo selection geneDepositorInsertmammalian-codon-optimized Flpe recombinase fused with ERT2
UseSynthetic BiologyTagsERT2ExpressionMammalianMutationPromoterCAGAvailable sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-HaloTag-Puromycin
Plasmid#167208PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with PuromycinDepositorTypeEmpty backboneUseCRISPRTagsHaloTagExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only