Addgene

We narrowed to 20,532 results for: crispr

Showing: 2421 - 2440 of 20532 results

Results per page:

pSAG1::CAS9-U6::sg290860-6

Plasmid

#59855

Purpose

Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.

Depositor

David Sibley

Article

Behnke et al Eukaryot Cell. 2014 Dec 5. pii: EC.00229

Insert

CRISPR sg290860-6

Use

CRISPR; ; toxoplasma gondii

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Sox1/2/3-2373/+3>nls::Cas9::nls

Plasmid

#59989

Purpose

Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Insert

nls::Cas9::nls

Use

CRISPR

Tags

Expression

Mutation

Reverted mutations from dCas9 to wiltdtype

Promoter

Ciinte.Sox1/2/3 (SoxB1) -2373/+3

Availability

Academic Institutions and Nonprofits only

Enlarge
pBlu/gRNA

Plasmid

#59188

Purpose

Serves as a shuttle vector for target oligo before insertion into Cas9 destination vector

Depositor

Robert Stupar

Article

Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

Insert

Guide RNA Cassette

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

U6 (Arabidopis)

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS SK mCherryROSAbsr

Plasmid

#54321

Purpose

Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.

Depositor

Silvestro Conticello

Article

Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

Insert

mCherry and BlasticidinS-resistance

Use

CRISPR, Mouse Targeting, and TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pHsp70-Cas9

Plasmid

#45945

Purpose

The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.

Depositor

Jill Wildonger, Melissa Harrison, Kate O'Connor-Giles

Article

Gratz et al Genetics. 2013 May 24.

Insert

Codon optimized Cas9

Use

CRISPR

Tags

3x FLAG

Expression

Mutation

Silent change from C to A at bp 834 of insert

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

Plasmid

#41863

Purpose

This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Zebrafish-gRNA-0006

Plasmid

#42246

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

gRNA-rgs4 (rgs4 Zebrafish)

Use

CRISPR; Zebrafish expression

Tags

Expression

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only
Zebrafish-gRNA-0008

Plasmid

#42248

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

gRNA-tia1l (tia1l Zebrafish)

Use

CRISPR; Zebrafish expression

Tags

Expression

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only
Zebrafish-gRNA-0009

Plasmid

#42249

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

gRNA-tph1a (tph1a Zebrafish)

Use

CRISPR; Zebrafish expression

Tags

Expression

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only
DR274

Plasmid

#42250

Purpose

guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR; Zebrafish expression

Tags

Expression

Worm

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2

Plasmid

#74010

Purpose

Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter

Depositor

Filippo Del Bene

Article

Di Donato et al Genome Res. 2016 Mar 8.

Inserts

5xUAS
Cas9
Cre
U6 promoter

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2K-CAGGS-U6-sgRNA-M5-IRES-CFP

Plasmid

#114728

Purpose

sgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc gene

Depositor

Martine Roussel

Article

Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

Insert

sgRNA M5

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-EF1adCas9VP64T2ABsd-W

Plasmid

#112919

Purpose

Lentiviral vector expressing Lentiviral vector expressing Lentiviral vector expressing dCas9VP64 under the control of the long human EF1a promoter

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

dCas9VP64-T2A-Blast

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human EF1a promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEmCherry2

Plasmid

#118413

Purpose

Red fluorescent reporter for assessing CRISPR gRNA activity

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

mCherry

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
p3s-Sniper-Cas9

Plasmid

#113912

Purpose

Expresses Sniper-Cas9 with CMV promoter

Depositor

Jungjoon Lee

Article

Lee et al Nat Commun. 2018 Aug 6;9(1):3048. doi: 1

Insert

Sniper-Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCasPA

Plasmid

#113347

Purpose

Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

Depositor

Quanjiang Ji

Article

Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pnCasPA-BEC

Plasmid

#113349

Purpose

A cytidine deaminase-mediated base-editing plasmid in Pseudomonas species

Depositor

Quanjiang Ji

Article

Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pMH-Cas9-gate

Plasmid

#113742

Purpose

Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving hygromycin for selection in plants.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

Cas9

Use

CRISPR; Gateway destination vector

Tags

Expression

Mutation

Promoter

Z. Mays ubiquitin promoter, 35S promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pZeo-Cas9-gate

Plasmid

#113744

Purpose

Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving Zeocin for selection in plants.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

Cas9

Use

CRISPR; Gateway destination vector

Tags

Expression

Mutation

Promoter

Z. Mays ubiquitin promoter, 35S promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
p2CT-His-MBP-Lwa_Cas13a_WT

Plasmid

#91865

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LwaCas13a

Use

CRISPR

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge