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Plasmid#25372DepositorInserthMCL1(S159A) (MCL1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationchanged serine 159 to alaninePromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLentiGuide_GFP_sgEPAS1#2
Plasmid#183120PurposepLentiGuide modified to express GFP and an sgRNA targeting EPAS1DepositorInsertEPAS1-2
UseLentiviralTagsExpressionMutationPromoterU6Available sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCXIN-EF1a-mNeonGreen-P2A-Cas9
Plasmid#189807PurposeRetroviral delivery of Cas9DepositorInsertCas9
UseRetroviralTagsExpressionMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTU2S-b (p15A origin)
Plasmid#74089PurposeSecondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 3 TU's with secondary module and p15A origin
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterNoneAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
YFP-PCAF-C2
Plasmid#134572PurposeExpresses YFP-PCAF fusion proteinDepositorInsertPCAF (Kat2b Mouse)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
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AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D
Plasmid#52521PurposeCo-expresses HA tagged channelrhodopsin and hM4D from a Cre-dependent virusDepositorInsertChR2-2a-hM4D
UseAAV and Cre/LoxTags2xHAExpressionMutationPromoterAvailable sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
KTK_251
Plasmid#180546PurposeEntry vector containing mScarletDepositorInsertmScarlet
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-mios in pRK5
Plasmid#154212PurposeN-terminal HA-tagged Mios cDNA expression vector for transfectionsDepositorInsertMios
UseTagsHA tagExpressionMammalianMutationcDNA sequence has been codon optimized using huma…PromoterCMVAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
YFP-POP5-C1
Plasmid#134543PurposeExpresses YFP-POP5 fusion proteinDepositorInsertPOP5 (POP5 Human)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sfGFP-DQNAT
Plasmid#198210PurposepJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing an optimal DQNAT sequon followed by a 6x-His tagDepositorInsertsfGFP_DQNAT
UseTags6x His Tag and DQNAT glycosylation tagExpressionBacterialMutationPromoterT7Available sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTU2-A-RFP (colE1 origin)
Plasmid#74093PurposepTU2-A backbone, with RFP negative selection and colE1 (medium-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 4 TU's with RFP and colE1 origin of replication
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterR0010 Const. Promoter controlling RFPAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNC1011
Plasmid#42898DepositorInsert3 tandem copies of GFP*
UseYeast integratingTagsExpressionMutationchanged Phenylalanine 64 to Leucine, Serine 65 t…PromoterNoneAvailable sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
CMV-H3.3-YFP
Plasmid#8693DepositorInsertH3.3A (His3.3A Fly)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
YFP-Rpp30-C1
Plasmid#134547PurposeExpresses YFP-Rpp30 fusion proteinDepositorInsertRpp30 (RPP30 Human)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166-iSpyMac
Plasmid#149553PurposeGateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMsDepositorInsertiSpyMacCas9
UseCRISPR; Gateway compatible zispymaccas9 entry clo…Tags3X FLAG, NLS and NLSExpressionPlantMutationR221K, N394K, Protospacer interacting domain form…PromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-FNIP1
Plasmid#49175PurposeMammalian expression of mouse folliculin interacting protein 1 (FNIP1) fused to GFPDepositorInsertFolliculin Interacting Protein 1 (Fnip1 Mouse)
UseTagsGFPExpressionMammalianMutationStop codon deletedPromoterCMVAvailable sinceDec. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCfB2988
Plasmid#63638PurposepCfB2988 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.URA3. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoternoneAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FullLength-TET1-LLAA(20)
Plasmid#124397PurposeFull-length mutant TET1 (L897A/L900A) used to PiggyBAC rescue the function of TET1/2 DKO cellsDepositorInsertFLAG-FullLength-TET1 with L897A/L900A mutations (TET1 Human)
UseTagsFlagExpressionMammalianMutationL897A/L900A mutations (full length protein is 213…PromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-mouse-retroCHMP3-HA
Plasmid#154178Purposeexpresses mouse retroCHMP3 in mammalian cellsDepositorInsertretroCHMP3
UseTagsHAExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only