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Plasmid#92078Purposeproduction of transgenic animal and recombination using flipaseDepositorInsertCMV-eGFP and TK-Neo between FRT3 and FRT
UsePiggybacTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
YFP-HA-DAT
Plasmid#90244PurposeExpresses YFP-HA-DAT in mammalian cellsDepositorInsertDAT (dopamine transporter) (SLC6A3 Human)
UseTagsExpressionMammalianMutationresidues 193–203 of human DAT were replaced in YF…PromoterCMVAvailable sinceJune 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEFmEGFP-mDia2
Plasmid#25407DepositorInsertmDia2 (Diaph3 Mouse)
UseTagsEGFP, His, and MycExpressionMammalianMutationEncodes mDia2PromoterAvailable sinceAug. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
UAS-Cre-T2A-miRFP670
Plasmid#193581PurposeFluorescent reporter for Gal4-induced Cre recombinationDepositorInsertmiRFP670
UseCre/LoxTagsT2AExpressionMutationPromoter5X UASAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pREF-GFP kan
Plasmid#122061Purposereporter plasmid for termination and terminator override to express GFP. Marked with URA3DepositorInsertsintergenic terminator from yeast IMD2
green fluorescent protein
UseTagsExpressionYeastMutationPromoterGAL1 and GAL1 comes in pYES2Available sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
RFP-FKBP-U2AF35
Plasmid#187969Purposeexpresses U2AF35 with N-terminal tags RFP and FKBPDepositorInsertU2AF35 (U2af1 Mouse)
UseCmv promoterTagsmRFP-FKBPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
RFP-FKBP-PRPF38A
Plasmid#187968Purposeexpresses PRPF38A with N-terminal tags RFP and FKBPDepositorInsertPrp38A (PRPF38A Synthetic, Human)
UseCmv promoterTagsmRFP-FKBPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET30b-5M SrtA with TEV site
Plasmid#86962PurposeExpresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tagDepositorInsertS.aureus SrtA 5M
UseTags6x Histidine tag and TEV siteExpressionBacterialMutationP94R, D160N, D165A, K190E, K196TPromoterT7Available sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip4
Plasmid#58925PurposeJIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6DepositorInsertMapk8ip4 (Spag9 Mouse)
UseTagsT7ExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET23a-His-hRanGAP1tail
Plasmid#53139PurposeE.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tagDepositorInsertRanGAP1 (RANGAP1 Human)
UseTagsHisExpressionBacterialMutationC-terminal fragment (amino acids 398-587)PromoterT7Available sinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Linear-2_PolyU(1)_Tornado-Corn
Plasmid#159487PurposeTests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.DepositorInsertTermination Module:Linear-2_PolyU(1)
UseTagsExpressionMammalianMutationPromoterU6-27Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO Plk4 KD-mCherry
Plasmid#80270Purposemammalian expression plasmid for kinase dead Plk4DepositorInsertPlk4 (PLK4 Human)
UseTagsmCherryExpressionMammalianMutationD154A kinase deadPromoterCMVAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR L1-vas2-Cre-L4
Plasmid#62301PurposeCre recombinase-expressing helper plasmid for Anopheles gene engineering, vasa promoterDepositorInsertCre recombinase under control of vasa promoter and terminator
UseTagsHA and SV40 NLSExpressionInsectMutationPromotervasaAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTol2_(-3kb)nrg1_typeII:GFP-CAAX
Plasmid#199394PurposepDEL63; transgenic construct to express GFP-CAAX under nrg1 type II regulatory region controlDepositorInserts(-3kb)nrg1 type II
GFP-CAAX
afp_3'_UTR
UseTol2 gateway destination vectorTagsExpressionMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
C514-E47: attB5r-Halotag-nostop-attB1r
Plasmid#162963PurposeGateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteinsDepositorInsertHalotag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSN444
Plasmid#108331PurposeExpresses Sat2 for Nourseothricin resistance selection of C. elegansDepositorInsertStreptothricin acetyl transferase 2
UseTagsExpressionWormMutationPromoterrps-27 promoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB6677
Plasmid#106155PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6632, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
GLN, Linear-3, PolyU(1)
Plasmid#166984PurposeTests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.DepositorInsertTermination module:PolyU(1)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB6684
Plasmid#106154PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6631, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB6682
Plasmid#106152PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6627, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only