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Plasmid#136973PurposeGoldengate/Greengate C-tag module, containing Venus fused to a flexible linkerDepositorInsertVenus
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGF_RT_FAST_RFP
Plasmid#136979PurposeGoldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescenceDepositorInsertseed coat marker RFP
UseTagsExpressionBacterialMutationBsaI site domestication in pOLE1:OLE1-RFP 688 G &…PromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX458-tRNA-SpCas9
Plasmid#195183PurposeVector for transient Cas9 and EGFP expression. Small tRNA promoter for sgRNA cloning by GoldenGate.DepositorInsertCas9
UseCRISPRTagsExpressionMutationPromotertRNA-GlnAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
ttHsCas12a
Plasmid#182385PurposeGives high editing efficiency in barley.DepositorInsertttHsCas12a
UseCRISPR; Coding sequence onlyTagsExpressionMutationD156RPromoterNoneAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDSAR
Plasmid#62292Purposedocking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, DsRed RFP markerDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDSAY
Plasmid#62291Purposedocking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, YFP markerDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDSAP
Plasmid#62293Purposedocking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, Puromycin resistance markerDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDSAG
Plasmid#62289Purposedocking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, GFP markerDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDSARN
Plasmid#62295Purposedocking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, DsRed-NLS markerDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDSAT
Plasmid#62290Purposedocking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, mTurquoise CFP markerDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDSAYN
Plasmid#62294Purposedocking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, YFP-NLS markerDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSKB-
Plasmid#62540PurposeCloning vector to prepare modules flanked by appropriately designed BsaI sites for seamless assembly of multiple inserts by GoldenGate CloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSEED-sfGFP
Plasmid#219639PurposePermits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest methodDepositorInsertsfGFP SEED Cassette
UseTagsExpressionMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEED-ALFA:HA
Plasmid#219646PurposePermits GoldenGate assembly (BsmBI) of donor plasmids to knock-in ALFA and HA tags using the SEED/Harvest methodDepositorInsertALFA:HA SEED Cassette
UseTagsExpressionMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEED-sfGFP:ALFA
Plasmid#219642PurposePermits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP:ALFA using the SEED/Harvest methodDepositorInsertsfGFP:ALFA SEED Cassette
UseTagsExpressionMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEED-mCherry
Plasmid#219657PurposePermits GoldenGate (BsmBI) assembly of donor plasmids to knock-in mCherry using the SEED/Harvest methodDepositorInsertsfGFP SEED Cassette
UseTagsExpressionMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
JME4580
Plasmid#129659PurposeHPHex_CrisprCas9-yl_RFP: CAS9 vector with Hygromycinex marker for gRNA cloning using GoldenGateDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
JME4599
Plasmid#129660PurposeNATex_CrisprCas9-yl_RFP: CAS9 vector with Nourseothricin ex marker for gRNA cloning using GoldenGateDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
JME4472
Plasmid#129658PurposeURA3ex_CrisprCas9-yl_RFP: CAS9 vector with URA3ex marker for gRNA cloning using GoldenGateDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
ttAtCas12a+int
Plasmid#182384PurposeGives very high editing efficiency in barley. Also works in Brassica oleraceaDepositorInsertLbCas12a coding sequence with D156R and Arabidopsis introns
UseCRISPRTagsExpressionMutationD156R + 8 Arabidopsis introns addedPromoterNoneAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only