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Plasmid#180202PurposeExpresses FLAG-tagged Acanthoeca spectabilis NF-kB3 protein in mammalian cellsDepositorInsertAs-NF-kB3
UseTagsFLAG-tagged fusion proteinExpressionMammalianMutationaa 2-412PromoterCMVAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-Co-NF-kB-Cterm
Plasmid#180199PurposeExpresses FLAG-tagged Capsaspora NF-kB C-terminal sequences in mammalian cellsDepositorInsertCo-NF-kBCterm
UseTagsFLAG-tagged fusion proteinExpressionMammalianMutationaa 542-1224PromoterCMVAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pClgn-Lrrc23-FLAG
Plasmid#179921PurposeVector backbone. pClgn-promoter driven expression.DepositorInsertLrrc23 (Lrrc23 Mouse)
UseTagsFLAGExpressionMammalianMutationStop codon removedPromoterCalmeginAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAPV-FLAG-DsRed2-ER-5
Plasmid#170620PurposeExpresses FLAG-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after SP cleavage.DepositorInsertAPV-FLAG-DsRed2-ER-5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPV-FLAG-DsRed2-ER-5
Plasmid#170621PurposeExpresses FLAG-DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide after signal peptide cleavage.DepositorInsertEPV-FLAG-DsRed2-ER-5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
FG11F-C-FLAG-hNKD2
Plasmid#174330Purposeencodes human NKD2 cDNA with a C-terminal FLAG tag in lentiviral vector. Does not encode any fluorescence/resistance marker.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseLentiviralTagsFLAG TagExpressionMammalianMutationPromoterUbCAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
MO70-C-FLAG-hNKD2
Plasmid#174327Purposeencodes human NKD2 cDNA with a C-terminal FLAG tag in retroviral expression vector. Encodes GFP from IRES site and puromycin resistanceDepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseRetroviralTagsFLAG TagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR-K630A-K632A
Plasmid#171263PurposeMammalian expression of 3xFlag-tagged human androgen receptor Lysine mutant: AR-Lys630Ala-Lys632Ala (AR-K630A-K632A)DepositorInsert3xFlag-tagged human androgen receptor Lys630Ala-Lys632Ala mutant (AR Human)
UseTagsFlagExpressionMammalianMutationAR-Lys630Ala-Lys632Ala (AR-K630A-K632A)PromoterCMVAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Flag-stm2585
Plasmid#174397PurposeNative S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags.DepositorInsertsarA
UseTagsEGFP and FlagExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Flag-stm2585_L139*
Plasmid#174400PurposeS. Typhimurium gene stm2585 (sarA/steE) truncated after 139th amino acid, which removes the stretch homologous to gp130DepositorInsertsarA
UseTagsEGFP and FlagExpressionMammalianMutationL139* (Truncation after 139th amino acid in sarA)PromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Flag-stm2585_S130A
Plasmid#174399PurposeNative S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its GSK3-binding SxxxSP motif mutated to SxxxAPDepositorInsertsarA
UseTagsEGFP and FlagExpressionMammalianMutationS130APromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Flag-stm2585_Y167F
Plasmid#174398PurposeNative S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its STAT3-binding YxxQ motif mutated to FxxQDepositorInsertsarA
UseTagsEGFP and FlagExpressionMammalianMutationY167FPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-stm2585_Y167F
Plasmid#174392PurposeS. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with STAT3-binding YxxQ motif mutated to FxxQDepositorInsertstm2585
UseTagsFlagExpressionMammalianMutationTyr 167 changed to PhePromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-stm2585
Plasmid#174391PurposeS. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with N-terminal Flag tagDepositorInsertstm2585
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJC2-GPT1-3xFLAG
Plasmid#163446Purposelentiviral overexpression vector for human GPT1DepositorInsertGPT1 (GPT Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationSee: commentsPromoterCMVAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HisFlag-rUSP19CSdomains
Plasmid#155240Purposefor mammalian overexpression of the CS domains of rat USP19DepositorInsertUSP19 (CS domains) (Usp19 Rat)
UseTags6XHis-3XFlagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
P3X-FLAG-SCD5
Plasmid#157955Purposeexpress SCD5 geneDepositorInsertSCD5 (SCD5 Sus scrofa)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HisFlag-rUSP19CYT
Plasmid#155238Purposefor mammalian overexpression of the cytoplasmic isoform of rat USP19DepositorInsertUSP19 (Cytoplasmic isoform) (Usp19 Mouse, Rat)
UseTags6XHis-3XFlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ 2xFLAG-2xSTREP_BACH1_Y11A
Plasmid#159131PurposeExpresses BACH1 in mammalian cellsDepositorInsertBACH1 (BACH1 Human)
UseTags2X-FLAG _ 2X-STREPExpressionMammalianMutationY11APromoterCMVAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_FLAG-Mx1201 PylRS
Plasmid#154764Purposeplasmid with Mx1201 PylRS for amber suppression in transient or stable, piggybac-mediated, integrationDepositorInsertMx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
UseTagsFLAGExpressionMammalianMutationPromoterEF1Available sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only