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Plasmid#219747PurposeDVK_AF CIDAR vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of CMV promoter, for mammalian expressionDepositorInsertpCMV - nnLuz_v4 - tSV40
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK291
Plasmid#219748PurposeDVK_AF CIDAR vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 under control of CMV promoter, for mammalian expressionDepositorInsertpCMV - nnH3H_v2 - tSV40
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
TfR-SpyCatcher003(K31TAG)-sfGFP-MycTag-CTag
Plasmid#210021PurposeExpression of transferrin receptor transmembrane domain and photocaged SpyCatcher003(K31HCK)-sfGFP on mammalian cell surface.DepositorInsertTransferrin receptor transmembrane domain-SpyCatcher003(K31TAG)-superfolderGFP-MycTag-CTag (TFRC Synthetic, Human)
UseTagsMycTag, CTagExpressionMammalianMutationTransferrin receptor transmembrane domain with Y2…PromoterCMV promoterAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUt-mNF-GFP
Plasmid#199220PurposeLPUtopia-7 matching RMCE donor plasmid with GFP Negative-Feedback circuit (mNF-GFP). Use BlastR for positive selection and HSV-TK for negative selection.DepositorInserthTetR::P2A::EGFP
UseTagsEGFPExpressionMammalianMutationPromoterCMV D2ir promoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRA-D842V_EGFP-PEST_reporter
Plasmid#172598PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V mutant signaling.DepositorInsertmyc-PDGFRA-D842V (PDGFRA Synthetic, Human)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-D842VPromoterCMVAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRA-D842V+R914W_EGFP-PEST_reporter
Plasmid#172599PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V+R914W mutant signaling.DepositorInsertmyc-PDGFRA-D842V+R914W (PDGFRA Synthetic, Human)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-D842V+R914WPromoterCMVAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRA-K627R+R914W_EGFP-PEST_reporter
Plasmid#172600PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R+R914W mutant signaling.DepositorInsertmyc-PDGFRA-K627R+R914W (PDGFRA Synthetic, Human)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-K627R+R914WPromoterCMVAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRA-K627R_EGFP-PEST_reporter
Plasmid#172597PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R mutant signaling.DepositorInsertmyc-PDGFRA-K627R (PDGFRA Synthetic, Human)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-K627RPromoterCMVAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS193
Plasmid#197143PurposescCloneSelect C-to-T DNA barcode cloning backbone (EF1a promoter version)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationEGFP(M1V)PromoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
hPGK-ABE7.10-P2A-GFP
Plasmid#162511PurposeExpress ABE7.10-P2A-GFP by hPGK promoter in mammalian cellsDepositorInsertABE7.10-P2A-GFP
UseCRISPRTagsNLSExpressionMammalianMutationPromoterhPGKAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
EFS-ABE7.10-P2A-GFP
Plasmid#162510PurposeExpress ABE7.10-P2A-GFP by EFS promoter in mammalian cellsDepositorInsertABE7.10-P2A-GFP
UseCRISPRTagsNLSExpressionMammalianMutationPromoterEFSAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS191
Plasmid#197140PurposescCloneSelect C-to-T reporter encoding BC-C1 barcode (EF1a promoter version)DepositorInsertEGFP
UseLentiviralTagsExpressionMammalianMutationEGFP(M1V)PromoterEF1aAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_cAMPFIRE-H
Plasmid#182281PurposeMammalian expression of the cAMP sensor cAMPFIRE-H under a CMV promotor.DepositorInsertcAMPFIRE-H (cAMP sensor)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_cAMPFIRE-L
Plasmid#182279PurposeMammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor.DepositorInsertcAMPFIRE-L (cAMP sensor)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_cAMPFIRE-M
Plasmid#182280PurposeMammalian expression of the cAMP sensor cAMPFIRE-M under a CMV promotor.DepositorInsertcAMPFIRE-M (cAMP sensor)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF_PE2
Plasmid#199267PurposeMammalian expression of SpCas9 prime editor 2 under EF1α promoterDepositorInsertPE2-P2A-mp53DD
UseTagsExpressionMammalianMutationΔ40-903PromoterAvailable sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-mIgkc
Plasmid#127157PurposeExpression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), kappa constant regionDepositorInsertIgkc (Igkc Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.1-mIglc2
Plasmid#127156PurposeExpression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), lambda 2 constant regionDepositorInsertIglc2 (Iglc2 Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-mIghg2b
Plasmid#127155PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2b isotypeDepositorInsertIghg2b (Ighg2b Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
MICU-APEX2
Plasmid#79057PurposeA C-terminal APEX2 fusion to the human mitochondrial calcium uptake 1 protein MICU1DepositorInsertC-terminal APEX2 fused MICU1 (MICU1 Soybean, Human)
UseLentiviralTagsFLAGExpressionBacterial and MammalianMutationPromoterCMV immearly promotorAvailable sinceSept. 1, 2016AvailabilityAcademic Institutions and Nonprofits only