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Plasmid#203985PurposeCloning plasmid containing the Elu ES2 insert with a chloramphenicol resistance markerDepositorInsertElu ES2
UseTagsExpressionPlantMutationPromoterElu ES2Available sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRMTK-clo16
Plasmid#203986PurposeCloning plasmid containing the Elu ES3 insert with a chloramphenicol resistance markerDepositorInsertElu ES3
UseTagsExpressionPlantMutationPromoterElu ES3Available sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRMTK-clo18
Plasmid#203988PurposeCloning plasmid containing the Ppu ES2 insert with a chloramphenicol resistance markerDepositorInsertPpu ES2
UseTagsExpressionPlantMutationPromoterPpu ES2Available sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRMTK-clo19
Plasmid#203989PurposeCloning plasmid containing the Ppu ES3 insert with a chloramphenicol resistance markerDepositorInsertPpu ES3
UseTagsExpressionPlantMutationPromoterPpu ES3Available sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRMTK-clo22
Plasmid#203992PurposeCloning plasmid containing the Hro ES3 insert with a chloramphenicol resistance markerDepositorInsertHro ES3
UseTagsExpressionPlantMutationPromoterHro ES3Available sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRMTK-clo26
Plasmid#204000PurposeCloning plasmid containing the Bpi ES1 insert with a chloramphenicol resistance markerDepositorInsertBpi ES1
UseTagsExpressionPlantMutationPromoterBpi ES1Available sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRMTK-clo28
Plasmid#204002PurposeCloning plasmid containing the Bpi ES3 insert with a chloramphenicol resistance markerDepositorInsertBpi ES3
UseTagsExpressionPlantMutationPromoterBpi ES3Available sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRMTK-clo1
Plasmid#203919PurposeCloning plasmid containing the BHR1 origin insert with a chloramphenicol resistance markerDepositorInsertBHR1 Origin of Replication
UseTagsExpressionPlantMutationPromoterN/AAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_EF-act1_tr
Plasmid#202171PurposeLevel 0 plasmid containing a terminator sequence from gene 29812 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.DepositorInsertact1 truncated terminator
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX-6p-1_GST-DmKHC[1-421]-1xGFP
Plasmid#196973PurposeBacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and cleavable N-terminal GST tagDepositorInsertKinesin Heavy Chain (Khc Fly)
UseTagsGFP and GSTExpressionBacterialMutationPromotertacAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-1_GST-SNAP-DmKHC[1-421]
Plasmid#196976PurposeBacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with N-terminal SNAP tag and cleavable N-terminal GSTDepositorInsertKinesin Heavy Chain (Khc Fly)
UseTagsGST and SNAPExpressionBacterialMutationPromotertacAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
4A3.PBAD-repL(WT).J23115-gfp
Plasmid#197872PurposeArabinose induced expression of wildtype repL gene, which triggers DNA replication in cis and in trans, weak level of constitutive GFP expressionDepositorInsertspart kilA/repL
gfp
UseTagsExpressionBacterialMutationcontains only the 24 bp of 3'-end of kilA ge…PromoterBba_J23115 and PBADAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
4A3.PBAD-repL(AT2).J23115-gfp
Plasmid#197873PurposeArabinose induced expression of repL(AT2) gene, which triggers DNA replication in trans, weak level of constitutive GFP expressionDepositorInsertpart kilA/repL(AT2)
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVioCD53
Plasmid#191130PurposeExpression of vioC, truncated vioD and tdMCP form a J23105 constitutive promoterDepositorInsertsUseSynthetic BiologyTagsExpressionMutationC-terminal truncation from 264th amino acidPromoterJ23105 constitutive promoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYUBcLIC-GFP
Plasmid#183509PurposeThe plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in mycobacteria.DepositorTypeEmpty backboneUseTags10x His-tag and folding reporter GFPExpressionBacterialMutationPromoterT7 lacAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTC3cIfm
Plasmid#193778PurposeExpresses frame-shifted cI under the control of PLTetO-1 promoter, p15A origin of replication, Chloramphenicol selectionDepositorInsertFrame-shifted CI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLTetO-1Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET29a(+)::9xhis-Tse5
Plasmid#192962PurposeHeterologous expression of Tse5 in E. coli Lemo21 cells for purification of Tse5-CTDepositorInsertTse5
UseTags9xhis + TEV tagExpressionBacterialMutationPromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458_miR-290-295KO-gRNA3
Plasmid#172711PurposeCas9 with 5' gRNA for paired CRISPR KO of miR-290-295 clusterDepositorInsertmiR-290-295
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
OTUB1/pRetroX-IRES-DsRed#10
Plasmid#180279PurposePlasmid encoding the human protease OTUB1 without a tag. Polycistronic red fluorescent retroviral vector (unmodified from TAKARA BIO).DepositorInserthuman Otubain-1 (OTUB1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmH207in
Plasmid#176230PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed toward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only