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Plasmid#110405PurposeCRISPR/Cas9 vector with sgRNA for glutaminase KGA isoform C-Terminal Knock-in and puromycin resistanceDepositorInsertGLS glutaminase (GLS Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19 KGA HA5'-mKO2-P2A-Zeo-3'
Plasmid#110408PurposeHomology donor for glutaminase KGA isoform knock-in, mKO2-P2A-ZeoDepositorInsertGLS glutaminase (GLS Human)
UseCRISPRTagsExpressionMutationPromoternoneAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_MGST3
Plasmid#111670PurposeMAC-tagged gene expressionDepositorInsertMGST3 (MGST3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 BMPR1A
Plasmid#67105PurposeStandard for cell-free expression benchmarking.DepositorInsertBMPR1A (BMPR1A Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7-gRNA:Asip
Plasmid#60968PurposeExpression vector of rat Asip guide RNADepositorInsertAgouti signaling protein gRNA (Asip Rat)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
mPapaya1-Keratin14-N-18
Plasmid#56353PurposeLocalization: Cytokeratin, Excitation: 530, Emission: 541DepositorInsertKeratin14 (KRT14 Human)
UseTagsmPapaya1ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pEBG-hBEN L38&45P
Plasmid#22182DepositorInserthBEN (GTF2IRD1 Human)
UseTagsGST and HisExpressionMammalianMutationChanged Leucine 38 to Proline and changed Leucine…PromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEBB-GFP-hBEN L38&45P
Plasmid#22183DepositorInserthBEN (GTF2IRD1 Human)
UseTagsGFPExpressionMammalianMutationChanged Luecine 38 to Proline and Leucine 45 to P…PromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MARK2
Plasmid#23404DepositorInsertMARK2 (MARK2 Human)
UseGateway donor vectorTagsExpressionMutationaa1-399PromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
Anti-Kv2.1 K+ channel [K89/34R-2b]
Plasmid#199404PurposeMammalian expression plasmid of Anti-Kv2.1 K+ channel [K89/34R-2b] (Rat). Derived from hybridoma K89/34.DepositorInsertAnti-Kv2.1 K+ channel (Rat) recombinant mouse monoclonal antibody (Kcnb1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
SOAP-COMP-blac-flag-his
Plasmid#110997PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsertsecreted ookinete adhesive protein (SOAP) (PF3D7_1404300 Synthetic)
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-lyso-pHluorin
Plasmid#70113PurposeExpression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidificationDepositorInsertlyso-pHluorin (Cd63 Aequorea victoria, Mouse)
UseTagsHA-Tag and pHluorinExpressionMammalianMutationPromoterCMVAvailable sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
TetO-Ascl1-puro
Plasmid#97329PurposeGenerating lentiviruses to express Ascl1 and puromycin resistanceDepositorInsertAscl1 (Ascl1 Mouse)
UseLentiviralTagsExpressionMutationPromoterTET-ONAvailable sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet_Giantin_C1
Plasmid#85048PurposeIn vivo visualization of the Golgi apparatus (can be used for colocalization studies)DepositorInsertGiantin (GOLGB1 Human)
UseTagsExpressionMammalianMutationaa 3131-3259PromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Fv-hCaspase 8-2A-GFP
Plasmid#82712Purposeexpress human caspase 8 in mammalian cellsDepositorInsertFv-Caspase 8-2A-GFP (CASP8 Human)
UseRetroviralTagsFKBP dimerization domainExpressionMammalianMutationdeleted amino acids 1-215 of caspase 8PromoterLTRAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 CB1
Plasmid#13391PurposeExpresses mouse cannabinoid receptor 1 (CB1) in mammalian cellsDepositorInsertCB1 (Cnr1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
p6345 MSCV-CMV-Flag-HA-Brd4 1-722
Plasmid#31352DepositorInsertBrd4 1-722 (BRD4 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutation*see commentsPromoterAvailable sinceOct. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
MSP10-bio
Plasmid#47719PurposeExpresses enzymatically monobiotinylated full-length MSP10 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised MSP10
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-Caveolin-C-10
Plasmid#55468PurposeLocalization: Membrane, Excitation: 462, Emission: 492DepositorInsertNM_001753 (CAV1 Human)
UseTagsmTFP1ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only