Skip to main content
Addgene
Showing: 2381 - 2400 of 158209 results

  2. pSBtet-RH

    Plasmid
    #60500
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and hygromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60500-plasmid-map-sequence-id-97547

    Enlarge

  3. TFORF3427

    Plasmid
    #144903
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFIB (NFIB Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    May 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    144903-plasmid-map-sequence-id-392202

    Enlarge

  6. TFORF3005

    Plasmid
    #144481
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SMAD4 (SMAD4 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    144481-plasmid-map-sequence-id-407356

    Enlarge

  7. pSBtet-BP

    Plasmid
    #60496
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of BFP, rtTA and puromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60496-plasmid-map-sequence-id-97551

    Enlarge

  11. TFORF2913

    Plasmid
    #143668
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFIB (NFIB Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143668-plasmid-map-sequence-id-400483

    Enlarge

  12. LYSO-LRRK2

    Plasmid
    #193582
    Purpose
    Expresses human full-length LRRK2 with a lysosomal targeting sequence from LAMTOR1 (1-39aa) and 3xFLAG on the N-terminus
    Depositor
    Mark Cookson
    Article
    Kluss et al Proc Natl Acad Sci U S A. 2022 Oct 25;11
    Insert
    Leucine-rich repeat kinase 2 (LRRK2 Human)
    Use
    Tags
    LAMTOR1(1-39aa) and 3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    193582-plasmid-map-sequence-id-382173

    Enlarge

  16. pMSR0

    Plasmid
    #161984
    Purpose
    Allele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile ribotype 027 and other ribotypes. Uses inducible expression of CD2517.1 toxin as a counter-selection marker
    Depositor
    Olga Soutourina
    Article
    Peltier et al Commun Biol. 2020 Nov 27;3(1):718. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    161984-plasmid-map-sequence-id-321926

    Enlarge

  17. pS148∙CsR

    Plasmid
    #197858
    Purpose
    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria
    Depositor
    Susanne Häußler
    Article
    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41
    Insert
    gRNA scaffold - Ap/Cb resistance cassette - incP oriT
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    PEM7
    Available since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197858-plasmid-map-sequence-id-393255

    Enlarge

  18. pSBtet-BH

    Plasmid
    #60499
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of BFP, rtTA and hygromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60499-plasmid-map-sequence-id-97548

    Enlarge

Showing: 2381 - 2400 of 158209 results