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Plasmid#63147PurposeKan marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only
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pFA6a-A1:hphMX4
Plasmid#134425PurposePCR-based C-terminal A1 tagDepositorInsertA1
UsePcr-based tagging in yeastTagsExpressionMutationPromoterAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-S6:hphMX4
Plasmid#134433PurposePCR-based C-terminal S6 tagDepositorInsertS6
UsePcr-based tagging in yeastTagsExpressionMutationPromoterAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXRT1
Plasmid#63151PurposeTrp1 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXRnMX
Plasmid#63153PurposeNat1 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-SRS:kanMX6
Plasmid#134442PurposePCR-based C-terminal SRS tagDepositorInsertSRS
UsePcr-based tagging in yeastTagsExpressionMutationPromoterAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-SRS:HIS3MX6
Plasmid#134443PurposePCR-based C-terminal SRS tagDepositorInsertSRS
UsePcr-based tagging in yeastTagsExpressionMutationPromoterAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-A1:HIS3MX6
Plasmid#134427PurposePCR-based C-terminal A1 tagDepositorInsertA1
UsePcr-based tagging in yeastTagsExpressionMutationPromoterAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-S6:HIS3MX6
Plasmid#134435PurposePCR-based C-terminal S6 tagDepositorInsertS6
UsePcr-based tagging in yeastTagsExpressionMutationPromoterAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
SLAC1-FL
Plasmid#117100Purposeprotein expression in yeastDepositorInsertSLAC1-FL
UseTagsHisExpressionYeastMutationPromoterAOXAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS316-4M5.3
Plasmid#45831DepositorInsert4M5.3
UseTagsFLAGExpressionYeastMutationPromoterGAL1Available sinceJune 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRS314-4M5.3
Plasmid#45830DepositorInsert4M5.3
UseTagsFLAGExpressionYeastMutationPromoterGAL1Available sinceJune 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
IpO
Plasmid#48233PurposeBase vector for PCR-based DNA transplant in yeast. DNA segments to be transplanted into the yeast genome are cloned into the MCS.DepositorInsertsURA3 3' fragment
URA3 5' fragment
UseRoutine cloning vectorTagsExpressionMutation-242 upstream to URA3 ORF +495 and URA3 ORF from …PromoterURA3 promoter and noneAvailable sinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yETSapphire-CaUra3
Plasmid#44650DepositorInsertTurbo Sapphire
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPL5071_TEF1*-Cre_URA3
Plasmid#60930PurposeConstitutive expression vector for Cre-recombinase containing URA3 selection marker.DepositorInsertCre
UseTagsDriven from a mutant TEF1 promoter with 0.16x act…ExpressionYeastMutationPromotermutant TEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
HO-hisG-URA3-hisG-poly-HO
Plasmid#51663Purposeyeast plasmid for integration of target sequence at HO locus, URA3 selectionDepositorTypeEmpty backboneUseYeast integrationTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymNeongreen-SpHis5
Plasmid#125704PurposeYeast tagging vector to create a gene fusion with yeast optimized mNeongreen with SpHIS5 selectionDepositorInsertymNeongreen
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS42H_PTEF1_dCas9_TCYC1
Plasmid#177155PurposeExpresses dCas9 in yeast cellsDepositorInsertNuclease-null Cas9
UseTagsSV40 NLSExpressionYeastMutationPromoterTEF1 promoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
HO-pMET-poly-KanMX4-HO
Plasmid#51665Purposeyeast plasmid for integration of target sequence at HO locus, Kan selection, pMET inducible promoterDepositorTypeEmpty backboneUseYeast integrationTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJYDC1
Plasmid#162458PurposeYeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter, eUnaG2 bilirubin dependent green-yellow fluorescent protein, is fused to the NterminusDepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsHA tag, HDEL sequence, eUnaG2 fluorescent protein…ExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only