Addgene

We narrowed to 981 results for: ubiquitin plasmid

Showing: 221 - 240 of 981 results

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mCherry-p62-LIR-mutant (LIRAAAA)

Plasmid

#187981

Purpose

Plasmid for the expression of a p62 mutant (LIR mutant: p62 3xDW + silent mutation). Internal reference: SMC528

Depositor

Sascha Martens

Article

Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

Insert

p62 (NUP62 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

DDDW335-338AAAA (LIR mutant), silent mutation siR…

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCW57.1-Tet-UPF1S124A/N138A/T139A (pRKB268)

Plasmid

#99144

Purpose

Inducible lentiviral construct to express FLAG-tagged UPF1S124A/N138A/T139A

Depositor

Robert Bradley

Article

Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

Insert

UPF1 (UPF1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

First 127 codons are optimized to reduce GC conte…

Promoter

pTight

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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p667-UBC-GFP-V5_IDG-K

Plasmid

#135242

Purpose

Gateway destination clone of GFP (as control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

GFP-V5

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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phage - ubc - nls - mcp - egfp - BirA*

Plasmid

#131134

Purpose

BirA* expressed as a protein fusion with MS2 coat protein (MCP) monomer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

Depositor

Ralf Jansen

Insert

NLS-HA-MCP-EGFP-BirA*

Use

Lentiviral

Tags

NLS, HA

Expression

Mammalian

Mutation

BirA* = BIrA >R118G

Promoter

human ubiquitin C (UBC)

Available since

Aug. 30, 2019

Availability

Academic Institutions and Nonprofits only

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phage - ubc - nls - 2xmcp - mCherry - BirA*

Plasmid

#131136

Purpose

BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and mCherry; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

Depositor

Ralf Jansen

Insert

NLS-HA-2xMCP-mCherry-BirA*

Use

Lentiviral

Tags

NLS, HA

Expression

Mammalian

Mutation

BirA* = BIrA >R118G

Promoter

human ubiquitin C (UBC)

Available since

Aug. 30, 2019

Availability

Academic Institutions and Nonprofits only

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p663-UBC-V5-dsRed_IDG-K

Plasmid

#135262

Purpose

Gateway destination clone of dsRed (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

V5-dsRed

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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USP37

Plasmid

#36898

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

USP37 (USP37 Human)

Use

Tags

His tag with thrombin cleavage

Expression

Bacterial

Mutation

contains amino acid residues 4-125 only

Promoter

Available since

June 21, 2012

Availability

Industry, Academic Institutions, and Nonprofits
p667-UBC-hcRed-V5_IDG-K

Plasmid

#135239

Purpose

Gateway destination clone of hcRed (as control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

hcRed-V5

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-V5-eGFP_IDG-K

Plasmid

#135265

Purpose

Gateway destination clone of eGFP (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

V5-eGFP

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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p667-UBC-GFP-V5_IDG-K

Plasmid

#135242

Purpose

Gateway destination clone of GFP (as control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

GFP-V5

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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phage - ubc - nls - mcp - egfp - BirA*

Plasmid

#131134

Purpose

BirA* expressed as a protein fusion with MS2 coat protein (MCP) monomer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

Depositor

Ralf Jansen

Insert

NLS-HA-MCP-EGFP-BirA*

Use

Lentiviral

Tags

NLS, HA

Expression

Mammalian

Mutation

BirA* = BIrA >R118G

Promoter

human ubiquitin C (UBC)

Available since

Aug. 30, 2019

Availability

Academic Institutions and Nonprofits only

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phage - ubc - nls - 2xmcp - mCherry - BirA*

Plasmid

#131136

Purpose

BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and mCherry; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

Depositor

Ralf Jansen

Insert

NLS-HA-2xMCP-mCherry-BirA*

Use

Lentiviral

Tags

NLS, HA

Expression

Mammalian

Mutation

BirA* = BIrA >R118G

Promoter

human ubiquitin C (UBC)

Available since

Aug. 30, 2019

Availability

Academic Institutions and Nonprofits only

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p663-UBC-V5-dsRed_IDG-K

Plasmid

#135262

Purpose

Gateway destination clone of dsRed (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

V5-dsRed

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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USP37

Plasmid

#36898

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

USP37 (USP37 Human)

Use

Tags

His tag with thrombin cleavage

Expression

Bacterial

Mutation

contains amino acid residues 4-125 only

Promoter

Available since

June 21, 2012

Availability

Industry, Academic Institutions, and Nonprofits
p667-UBC-hcRed-V5_IDG-K

Plasmid

#135239

Purpose

Gateway destination clone of hcRed (as control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

hcRed-V5

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-V5-eGFP_IDG-K

Plasmid

#135265

Purpose

Gateway destination clone of eGFP (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

V5-eGFP

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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USP5

Plasmid

#25299

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

USP5.001.835 (USP5 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 16, 2010

Availability

Industry, Academic Institutions, and Nonprofits
PP-UEV1A

Plasmid

#25111

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

PFC0255c:C34858

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2010

Availability

Industry, Academic Institutions, and Nonprofits
PP-UBC13 (3E95)

Plasmid

#25131

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

PFE1350c:C34878

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2010

Availability

Industry, Academic Institutions, and Nonprofits
USP5

Plasmid

#25299

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

USP5.001.835 (USP5 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 16, 2010

Availability

Industry, Academic Institutions, and Nonprofits