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Plasmid#165509PurposeUsed for site-specific cleavage of proteins. TEV cleaves the protein consensus sequence EXXYXQ^S/GDepositorInsertTEV protease
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
FKBP-C-TEV 219
Plasmid#100096PurposeSNIPer plasmid, C terminal TEV fused to FKBPDepositorInsertC-TEV
UseRetroviralTagsFKBP and MycExpressionMammalianMutationC terminal domainPromoterCMVAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
His-Tev-Nedd4L
Plasmid#186806PurposeCodon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-Nedd4L.DepositorInsertHis-Tev-Nedd4L (NEDD4L Human)
UseTagsHis6-TevExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKK-MBP-TEV
Plasmid#105771PurposeExpression of your protein of interest in fusion with MBP at the N-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).DepositorTypeEmpty backboneUseFlp-in competentTagsMBP-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
His-Tev-WWP2
Plasmid#186807PurposeCodon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-WWP2.DepositorInsertHis-Tev-WWP2 (WWP2 Human)
UseTagsHis6-TevExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
H6GB1tevP53(1-303)IntN
Plasmid#200312PurposeBacterial expression of P53 NTAD-DBD for segmental labelingDepositorInsertp53 (TP53 Human)
UseTagsHis6GB1tev and designed intein NExpressionBacterialMutationM133L, V203A, N239Y, N268DPromoterT7Available sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-EGFP
Plasmid#105793PurposeExpression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 8885998, 8805248). EGFP is not truly monomeric (PMID: 11988576, 22869113)DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-EGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-CyOFP1-TEV
Plasmid#105782PurposeExpression of your protein of interest in fusion with orange fluorescent protein at the N-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.DepositorTypeEmpty backboneUseFlp-in competentTagsCyOFP1-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-mCherry-TEV
Plasmid#105779PurposeExpression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 15558047). mCherry descends from the first truly monomeric red FP mRFP1.DepositorTypeEmpty backboneUseFlp-in competentTagsmCherry-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mCherry
Plasmid#105796PurposeExpression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 15558047). mCherry descends from the first truly monomeric red FP mRFP1.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-mClover3-TEV
Plasmid#105778PurposeExpression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).DepositorTypeEmpty backboneUseFlp-in competentTagsmClover3-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
V2-MESA-35F-Tev
Plasmid#84503PurposeMESA protease chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, and Tev proteaseDepositorInsertV2-MESA-35F-Tev
UseAAVTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
mtagBFP2-CaM-uTEVp
Plasmid#163033PurposeFLiCRE protease for neuronal expressionDepositorInsertBFP-CaM-uTEVp
UseTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETZZtevPP7CP(C68A,C73A)
Plasmid#27548DepositorInsertPseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)
UseTagsTEV protease cleavage site, six histidine tag, an…ExpressionBacterialMutationchanged two cysteine to alanine (C68A,C73A) to in…PromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pH6-TEV-mCherry
Plasmid#132430PurposeExpresses His6-tagged mCherry in E. coli using T7 promoterDepositorInsertmCherry
UseTagsN-terminal His6 tag with TEV protease siteExpressionBacterialMutationCodon optimizedPromoterT7Available sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
ECFP-TevS-YPET
Plasmid#100097PurposeFRET-based ECFP-YPET reporter that is cleaved by TEVDepositorInsertECFP-TevS-YPET
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDipA-TEV-His12
Plasmid#137042Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi DipA with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66790.1 (BB_0418 Borrelia burgdorferi B31)
UseTagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-ProteinA
Plasmid#105788PurposeExpression of your protein of interest in fusion with two IgG binding domains of Staphylococcus aureus protein A at the C-terminus (cleavable by TEV) (PMID: 3507693, 10504710). Protein purification.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-ProteinAExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
V1-MESA-45F-Tev
Plasmid#84501PurposeMESA protease chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, and Tev proteaseDepositorInsertV1-MESA-45F-Tev
UseAAVTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-NES
Plasmid#171006PurposeHiLITR protease with C-terminal NNES (Cytoplasmic)DepositorInsertEGFP-uTEV1-NES
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only