Addgene

We narrowed to 2,908 results for: sam

Showing: 221 - 240 of 2908 results

Results per page:

Sort results by:

CAG::PSAMQ79G,Q139G:5HT3HC-IRES-GFP

Plasmid

#32474

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAMQ79G,Q139G:5HT3 HC

Use

Tags

IRES-EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 10, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-LSAMP-COMP5AP-AviTag-9xHis

Plasmid

#157288

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

LSAMP (LSAMP Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-ESAM-COMP5AP-AviTag-9xHis

Plasmid

#157218

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

ESAM (ESAM Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-ESAM-Fc(DAPA)-AviTag-6xHis

Plasmid

#156654

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

ESAM (ESAM Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV SYN flex PSAM4 GlyR IRES EGFP

Plasmid

#119741

Purpose

Chemogenetics Cre-dependent expression plasmid

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Has Service

AAV5 and AAV9

Insert

PSAM4 GlyR IRES eGFP (CHRNA7 Synthetic, Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

L131G, Q139L, Y217F

Promoter

synapsin

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 5-1

Plasmid

#177920

Purpose

Expresses ratiometric Squash-SAM sensor 5-1 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Tornado-F30-Broccoli-Squash-SAM-sensor 5-1

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6+27

Available since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 4-2

Plasmid

#177919

Purpose

Expresses ratiometric Squash-SAM sensor 4-2 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Tornado-F30-Broccoli-Squash-SAM-sensor 4-2

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6+27

Available since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
rAAV-syn::FLEX-rev::PSAML141F:GlyR-IRES-GFP

Plasmid

#32479

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAML141F-GlyR-IRES-GFP

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

IRES-EGFP

Expression

Mutation

Promoter

Synapsin

Available since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pUPD2 sgRNA:scaffold tetraloop MS2 aptamer SAM (GB1436)

Plasmid

#160570

Purpose

A version of scaffold sgRNA with the sequence of MS2 aptamer inside the tetraloop of the scaffold

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

sgRNA:scaffold tetraloop MS2 aptamer SAM

Use

CRISPR

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
rAAV-syn::FLEX-rev::PSAML141F,Y115F:GlyR-IRES-GFP

Plasmid

#32481

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAML141F,Y115F-GlyR

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

IRES-EGFP

Expression

Mutation

Promoter

Synapsin

Available since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
rAAV-syn::FLEX-rev::PSAMQ79G,Q139G:5HT3HC-IRES-GFP

Plasmid

#32475

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAMQ79G,Q139G-5HT3HC

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

IRES-EGFP

Expression

Mutation

Promoter

Synapsin

Available since

Dec. 12, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
rAAV-syn::FLEX-rev::PSAML141F,Y115F:5HT3HC-IRES-GFP

Plasmid

#32477

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAML141F,Y115F-5HT3HC

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

IRES-EGFP

Expression

Mutation

Promoter

Synapsin

Available since

Jan. 12, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
Human SAM library in lentiSAMv2 backbone

Pooled Library

#1000000078

Purpose

Genome-wide CRISPR gRNA pooled library for activation of human genes

Depositor

Feng Zhang

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
Human SLC SAM Library

Pooled Library

#132561

Purpose

Expresses gRNAs for activation of human Solute Carrier family genes

Depositor

Giulio Superti-Furga

Article

Rebsamen et al Life Sci Alliance. 2022 Sep 16;5(11):e20

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

May 18, 2020

Availability

Academic Institutions and Nonprofits only
Human SAM library in lenti sgRNA(MS2)_puro backbone

Pooled Library

#1000000074

Purpose

Genome-wide CRISPR gRNA pooled library for activation of mouse genes

Depositor

Feng Zhang

Available since

March 17, 2016

Availability

Academic Institutions and Nonprofits only
Human SAM Library in lenti sgRNA(MS2)_zeo backbone

Pooled Library

#1000000057

Purpose

Genome-wide CRISPR gRNA pooled library for activation of human genes

Depositor

Feng Zhang

Available since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only
Mouse SAM Library in lenti sgRNA(MS2)_puro optimized backbone

Pooled Library

#1000000075

Purpose

Genome-wide CRISPR gRNA pooled library for activation of mouse genes

Depositor

Feng Zhang

Available since

March 17, 2016

Availability

Academic Institutions and Nonprofits only
Human CRISPR lncRNA activation library (SAM) in lenti sgRNA(MS2)_zeo backbone

Pooled Library

#1000000106

Purpose

Human CRISPR Synergistic Activation Mediator (SAM) pooled library for the transcriptional activation of lncRNAs

Depositor

Feng Zhang

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only
pAEM7

Plasmid

#136422

Purpose

salL gene from S. tropica CNB-440 ligated into pHIS8

Depositor

Bradley Moore

Article

Eustaquio et al Nat Chem Biol. 2008 Jan;4(1):69-74. doi:

Insert

salL

Use

Tags

His8 tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
His8OphMA-21mer.

Plasmid

#189670

Purpose

His8 tagged OphMA with TEV cleavable C-terminus to generate 21mer and 18merNL.

Depositor

James Naismith, Markus Künzler

Article

Emmanuel et al bioRxiv 2022.07.21.500988

Insert

His8OphMATEV18mer

Use

Tags

His8 tagged

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge