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Plasmid#99659PurposeExpresses dSp Cas9 fused to truncated p65 activation domainDepositorArticleInsertdCas9
UseTagstruncated p65 activation domain (1-150)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
RPO41 deltaN100
Plasmid#205073PurposeRPO41 protein expressionDepositorInsertRPO41 (RPO41 Budding Yeast)
UseTagsExpressionBacterialMutationLacks N Terminal 100 amino acidsPromoterAvailable sinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
RPO41 deltaN380
Plasmid#205076PurposeRPO41 protein expressionDepositorInsertRPO41 (RPO41 Budding Yeast)
UseTagsExpressionBacterialMutationLacks N Terminal 380 amino acidsPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
RPO41 deltaN296
Plasmid#205075PurposeRPO41 protein expressionDepositorInsertRPO41 (RPO41 Budding Yeast)
UseTagsExpressionBacterialMutationLacks N Terminal 296 amino acidsPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
p09008_AF1-4+GFP5-7
Plasmid#173715PurposePlasmid backbone for the HyperXpress workflow containing new to nature hybrid GFP with strong fluorescence in E. coli cell free protein synthesis extracts.DepositorInsertshybrid GFP
mKate2
UseE. coli cell free protein synthesis extractsTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH776_pJH_LldR_PlldR-sfGFP.3opal
Plasmid#163735PurposeLactate-inducible expression of green fluorescent protein encoding three opal codonsDepositorInsertlldr gfp.3TGA
UseTagsExpressionBacterialMutationgfp with first three leucine codons mutated to am…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH806_pJH_LldR_Pcon-sfGFP.1TAG
Plasmid#163737PurposeConstitutive expression of green fluorescent protein encoding one stop codonDepositorInsertlldr gfp.1TAG
UseTagsExpressionBacterialMutationgfp.N39TAGPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH28_pBad_AraC_Para-sfGFP.3TAG
Plasmid#163723PurposeArabinose-inducible expression of green fluorescent protein encoding three amber codonsDepositorInsertaraC gfp.3TAG
UseTagsExpressionBacterialMutationgfp.N39TAG,N135TAG,Y151TAGPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH626_pJH_LldR_PlldR-sfGFP.1TAG
Plasmid#163725PurposeLactate-inducible expression of green fluorescent protein encoding one amber codonDepositorInsertlldr gfp.1TAG
UseTagsExpressionBacterialMutationgfp.N39TAGPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH627_pJH_LldR_PlldR-sfGFP.2TAG
Plasmid#163726PurposeLactate-inducible expression of green fluorescent protein encoding two amber codonsDepositorInsertlldr gfp.2TAG
UseTagsExpressionBacterialMutationgfp.N39TAG,Y135TAGPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH628_pJH_LldR_PlldR-sfGFP.3TAG
Plasmid#163727PurposeLactate-inducible expression of green fluorescent protein encoding three amber codonsDepositorInsertlldr gfp.3TAG
UseTagsExpressionBacterialMutationgfp.N39TAG,N135TAG,Y151TAGPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH721_pJH_LldR_PlldR-sfGFP.20TAG
Plasmid#163729PurposeLactate-inducible expression of green fluorescent protein encoding twenty amber codonsDepositorInsertlldr gfp.20TAG
UseTagsExpressionBacterialMutationgfp with all 20 leucine codons mutated to amberPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH726_pJH_LldR_PlldR-sfGFP.15TAG
Plasmid#163730PurposeLactate-inducible expression of green fluorescent protein encoding fifteen amber codonsDepositorInsertlldr gfp.15TAG
UseTagsExpressionBacterialMutationgfp with first 15 leucine codons mutated to amberPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH727_pJH_LldR_PlldR-sfGFP.10TAG
Plasmid#163731PurposeLactate-inducible expression of green fluorescent protein encoding ten amber codonsDepositorInsertlldr gfp.10TAG
UseTagsExpressionBacterialMutationgfp with first 10 leucine codons mutated to amberPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH774_pJH_LldR_PlldR-sfGFP.1opal
Plasmid#163733PurposeLactate-inducible expression of green fluorescent protein encoding one opal codonDepositorInsertlldr gfp.1TGA
UseTagsExpressionBacterialMutationgfp with first leucine codon mutated to amberPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH775_pJH_LldR_PlldR-sfGFP.2opal
Plasmid#163734PurposeLactate-inducible expression of green fluorescent protein encoding two opal codonsDepositorInsertlldr gfp.2TGA
UseTagsExpressionBacterialMutationgfp with first two leucine codons mutated to amberPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH578
Plasmid#172162PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD 3f2_v2); AraC
UseTagsExpressionBacterialMutationPylB(N61S, E84K, E122K, E178K, G188R, E251D) PylC…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH767
Plasmid#172172PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.WT]
UseTagsExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB]PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH857
Plasmid#172175PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.WTopt]
UseTagsExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBopt]PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH223
Plasmid#172151PurposepylBCD variant in pCDF backboneDepositorInsertPT7/lac (pylBCD 34B_sub-pop5); LacI
UseTagsExpressionBacterialMutationPylB(N61S, E178K, E251D) PylD(A252V)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only