-
Plasmid#228076PurposeMammalian expression of coiled coil segments (S1h-S3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertmCit, S1h, S3h
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCit(D1h-D3h)3-gs
Plasmid#228088PurposeMammalian expression of coiled coil segments (D1h-D3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertmCit, D1h, D3h
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEM-PH-citrine
Plasmid#131406PurposePurpose: synthesis of mRNA encoding a membrane marker. Insert: PH domain of the human phospholipase C delta 1 (PLCD1) fused with the yellow fluorescent protein mCitrineDepositorInsertPH
UseIn vitro transcriptionTagscitrine yellow fluorescent proteinExpressionMutationPromoterT7Available sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_B2AR-s-pep
Plasmid#47438PurposeFRET sensor for beta2-adrenergic receptorDepositorInsertbeta2-adrenergic receptor (ADRB2 Human)
UseTags10 nm ER/K helix, HA, mCerulean, mCitrine, and s-…ExpressionMammalianMutationPromoterpCMVAvailable sinceSept. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_B2AR-i-pep
Plasmid#47436PurposeFRET sensor for beta2-adrenergic receptorDepositorInsertbeta2-adrenergic receptor (ADRB2 Human)
UseTags10 nm ER/K helix, HA, i-pep, mCerulean, and mCitr…ExpressionMammalianMutationPromoterpCMVAvailable sinceSept. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
FRB_T2098L-VAMP3-mCherry
Plasmid#92429PurposeExpression of VAMP3 N-terminally conjugated to FRB and C-terminally conjugated to mCitrine.DepositorInsertVAMP3 (Vamp3 Mouse)
UseTagsFKBP rapamycin binding (FRB) domain of mTOR with …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_B2AR-no-pep
Plasmid#47439Purposecontrol FRET sensor for beta2-adrenergic receptorDepositorInsertbeta2-adrenergic receptor (ADRB2 Human)
UseTags10 nm ER/K helix, HA, mCerulean, mCitrine, and no…ExpressionMammalianMutationPromoterpCMVAvailable sinceSept. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_B2AR-q-pep
Plasmid#47437PurposeFRET sensor for beta2-adrenergic receptorDepositorInsertbeta2-adrenergic receptor (ADRB2 Human)
UseTags10 nm ER/K helix, HA, mCerulean, mCitrine, and q-…ExpressionMammalianMutationPromoterpCMVAvailable sinceSept. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBiex1-SYNAC
Plasmid#82710PurposeSynthetic soluble adenylyl cyclase FRET sensor and cAMP generatorDepositorUseTagsFLAG, mCerulean, and mCitrineExpressionBacterial and InsectMutationPromoterT7Available sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-OptoFGFR1-D387A
Plasmid#59778PurposeExpresses optoFGFR1 with mutation in PHR to suppress homointeraction, thus cannot be activated by lightDepositorInsertOptoFGFR1-D387A (FGFR1 Human, Mustard Weed)
UseTagsmCitrineExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-OptoFGFR1-Y766F
Plasmid#59777PurposeExpresses optoFGFR1 with mutation in PLC-gamma binding phosphotyrosine site of FGFR1DepositorInsertOptoFGFR1-Y766F (FGFR1 Human, Mustard Weed)
UseTagsmCitrineExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCY 3080-01
Plasmid#36226DepositorInsertyEmCitrine
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-yEmCitrine-kanMXExpressionMutationPromoterAvailable sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKT0211
Plasmid#8734DepositorInsertyECitrine A206R
UseTagsCodon optimized linkerExpressionYeastMutationyEGFP with S65G, V68L, Q69M, S72A, T203Y, A206RPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCY 3080-04
Plasmid#36228DepositorInsertTRP1
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-yEmCitrine-TRP1ExpressionMutationPromoterAvailable sinceAug. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCY 3080-06
Plasmid#36230DepositorInsertURA3
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-yEmCitrine-URA3ExpressionMutationPromoterAvailable sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCY 3080-07
Plasmid#36227DepositorInsertStreptoalloteichus hindustanus bleomycin (Sh ble)
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-yEmCitrine-ZeocinExpressionMutationPromoterAvailable sinceAug. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
Hamdoun lab FP fusion plasmids
Plasmid Kit#1000000033PurposeScreen fluorescent protein fusion expression in organisms or cells in which an exogenous mRNA can be injected and expressed, e.g. zebrafish, sea urchin, frog (Xenopus), or worm (C. elegans)DepositorAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only