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Plasmid#148432PurposeMammalian Expression of 5BoxB-polyA-MalatDepositorInsert5BoxB-polyA-Malat
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-GQ-RLuc-6xbsMS2-A95-Malat_AC
Plasmid#148426PurposeMammalian Expression of 6xbsMS2-A95-MalatDepositorInsert6xbsMS2-A95-Malat
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICH47811-pAtAct2::Cas9-nls::tSlATPase
Plasmid#160221PurposeCas9-nuclear localization, pACT2 promoter, SlATPase terminator transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 2 reverseDepositorInsertpAtACT2::Cas9-nls::tSlATPase
UseSynthetic Biology; Moclo toolkit level 1 acceptor…TagsExpressionPlantMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-EC1.45-human_min1-platypus_min2
Plasmid#173967PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertHuman minimal enhancer region 1 (min1) and platypus minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45
UseLuciferaseTagsExpressionMutationNonePromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
AtS/MAR10 insulator sequence (GB3459)
Plasmid#160639PurposepEGB3a2 AtS/MAR10DepositorInsertAtS/MAR10 insulator sequence
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD027 Reporter Template v1 (Near Spaced)
Plasmid#138751PurposeTemplate for COMET promoters, expresses EYFP reporter upon promoter activation.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD032 Reporter Template v1 (Mid Spaced)
Plasmid#138753PurposeTemplate for COMET promoters, expresses EYFP reporter upon promoter activation.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD033 Reporter Template v1 (Far Spaced)
Plasmid#138754PurposeTemplate for COMET promoters, expresses EYFP reporter upon promoter activation.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Fibronectin-human-mutated V2 O-Glycosylation site
Plasmid#120405Purposeenables eukaryotic expression of human plasma fibronectin in which the second O-glycosylation site was mutated from threonine to serineDepositorInsertFibronectin (FN1 Human)
UseTagsExpressionMammalianMutationContains complete variable region with a mutation…PromoterCMVAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)
Plasmid#128604PurposeExpresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.DepositorInsertsUseTagsExpressionMammalianMutationPromoterPcmvAvailable sinceAug. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-Rab18-sfGFP(N) HDR template
Plasmid#129415PurposeHDR tempalte for tagging of endogenous human RAB18 N-terminus with sfGFPDepositorInsertRAB18 HDR template (RAB18 Human)
UseCRISPR and TALEN; Endogenous tagging hdr templateTagssfGFPExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-3XpolyA-3XPC-miniCMV-mCherry-WPRE-insulator
Plasmid#168291Purposeoptimized miniCMV (OminiCMV)DepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterminiCMVAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO CMV-cGFP-mMALAT1_3' WT Sense
Plasmid#46834PurposeExpress cGFP transcript ending in the wildtype MALAT1 triple helixDepositorInsertCoral Green Fluorescent Protein (cGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
SOX9-T2A-H2B-EGFP repair template
Plasmid#167972PurposeSOX9 reporter repair templateDepositorInsertSRY-Box Transcription Factor 9 (SOX9 Human)
UseCRISPR; Donor templateTagsH2B-EGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-S28 histone 3 phosphorylation reporter-NLS
Plasmid#32787DepositorInsertCFP-14-3-3tau-H3 peptide-YFP
UseTagsCFP and YFPExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRL-TK-hFluc-CrPV-hRluc-6xbsMS2-Malat_AC
Plasmid#148443PurposeMammalian Expression of 6xbsMS2-MalatDepositorInsert6xbsMS2-Malat
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
nonphosphorylatable Mis12-targeted Aurora B FRET sensor
Plasmid#45229DepositorInsertnon phosphorylatable Mis12-targeted Aurora B FRET sensor
UseTagsExpressionMammalianMutationT-A mutation in substrate sequencePromoterAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-ACSL3-sfGFP(C) HDR template
Plasmid#129413PurposeHDR tempalte for tagging of endogenous human ACSL3 C-terminus with sfGFPDepositorInsertACSL3 HDR template (ACSL3 Human)
UseCRISPR and TALEN; Endogenous tagging hdr templateTagssfGFPExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC031 - RcrCas13a from Rhodobacter capsulatus R121
Plasmid#91921PurposeExpresses RcrCas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertRcrCas13a and guide expression cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC032 - RcdCas13a from Rhodobacter capsulatus DE442
Plasmid#91922PurposeExpresses RcdCas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertRcdCas13a and guide expression cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only