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Plasmid#114034PurposeProduction of baculovirus with LacI-LAPDepositorInsertLacI-LAP
UseTagsExpressionInsectMutationnonePromoterCMVAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Mel18 flag
Plasmid#1969DepositorInsertMel18 (Pcgf2 Mouse)
UseTagsflagExpressionInsectMutationPromoterAvailable sinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFasBac+GFP-CAMSAP2 CC
Plasmid#59040Purposefor baculovirus expression of CAMSAP2 CC in Sf9 cellsDepositorInsertCAMSAP2 CC domain (CAMSAP2 Human)
UseTagsHis and eGFPExpressionInsectMutationPromoterAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-EF09
Plasmid#52640Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-EF09
UseTagsExpressionMammalianMutationD366N mutation from GCaMP3 sequencePromoterT7Available sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-hDcr-K70A.
Plasmid#89145PurposeExpression of helicase mutant of human Dicer in Sf9 cellsDepositorInserthDicer (DICER1 Human)
UseTagsHis6-tevExpressionInsectMutationK70APromoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFasBac+GFP-CAMSAP3 CC
Plasmid#59042Purposefor baculovirus expression of mouse CAMSAP3 CC in Sf9 cellsDepositorInsertCAMSAP3 CC domain (Camsap3 Mouse)
UseTagsHis and eGFPExpressionInsectMutationPromoterAvailable sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
FASTK gRNA (BRDN0001144738)
Plasmid#77876Purpose3rd generation lentiviral gRNA plasmid targeting human FASTKDepositorInsertFASTK (FASTK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FASTK gRNA (BRDN0001145485)
Plasmid#77877Purpose3rd generation lentiviral gRNA plasmid targeting human FASTKDepositorInsertFASTK (FASTK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-EF15
Plasmid#52641Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-EF15
UseTagsExpressionMammalianMutationD431N/D433N/D435N mutated from GCaMP3PromoterT7Available sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFastbac-Flag-BRCA1
Plasmid#223228PurposeInsect expression construct for Flag-tagged BRCA1 protein production and purificationDepositorInsertBreast Cancer 1 (BRCA1 Human)
UseTagsFlagExpressionInsectMutationPromoterPolyhedrinAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-GST-TBK1
Plasmid#221331PurposeTBK1 protein overexpression in Sf9 insect cellsDepositorInsertTBK1 (Tbk1 Mouse)
UseTags6xHis-TEV-2xGST-TEVExpressionInsectMutationPromoterpolyhedrinAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
ER-FAST-IRES-iRFP670
Plasmid#216344PurposeFor testing localization of pFAST and iRFP670 proteins to endoplasmic reticulumDepositorInsertPRL_pFAST-IRES-CANX_iRFP670
UseTagsmyc for FAST and iRF670 proteinsExpressionMammalianMutationn/aPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NFAST(low)-Halo-ER
Plasmid#214419PurposeER localization of low-affinity NFAST fused to Halo-ERDepositorInsertRspA-N
UseTagsHaloExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
CFAST-Halotag-6xHp
Plasmid#214416PurposeLipid droplet localization of CFAST fused to Halotag-6xHpDepositorInsertdeletion
UseTagsCFAST-HaloExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-C17orf53-Strep
Plasmid#211446Purposegeneration of Bacmid for insect cell expression of C17orf53 C-terminally fused to Strep-TagDepositorInsertC17orf53 (HROB Human)
UseTagsStrep-TagExpressionInsectMutationPromoterAvailable sinceMarch 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-C17orf53-FLAG
Plasmid#211447Purposegeneration of Bacmid for insect cell expression of C17orf53 C-terminally fused to FLAG-TagDepositorInsertC17orf53 (HROB Human)
UseTagsFLAG-TagExpressionInsectMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-bullfrog saxiphilin
Plasmid#140595Purposeprotein expressionDepositorInsertsaxiphilin bullfrog
UseTagsGFP-His10ExpressionInsectMutationDeletion of M20 - please see depositor commentPromoterpolyhedrin promoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-M.Fas.1
Plasmid#196687PurposeRep/Cap plasmid for the production of M.Fas.1, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationTDALTTK insert between amino acids 588 and 589 of…Promoterp41Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-M.Fas.4
Plasmid#196690PurposeRep/Cap plasmid for the production of M.Fas.4, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationVVSDYTV insert between amino acids 588 and 589 of…Promoterp41Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-M.Fas.3
Plasmid#196689PurposeRep/Cap plasmid for the production of M.Fas.3, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationRVDPSGL insert between amino acids 588 and 589 of…Promoterp41Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only