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Plasmid#85126PurposeNSIII -Cm-PkaiB-luc+ (firefly) vector constructed by seamless cloning with Cm casette codon optimized for S7942. PkaiB-luc+ amplified from pAM2105.DepositorInsertPkaiB-luciferase
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM1956
Plasmid#40251DepositorInsertGFPmut2
UseCyanobacteria cloning vectorTagsExpressionMutationPromotern/aAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAM1954
Plasmid#40250DepositorInsertGFPmut2
UseCyanobacteria cloning vectorTagsExpressionMutationPromotern/aAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRL692
Plasmid#70284PurposeTn5-based plasmid for transposition in Synechococcus sp. strain PCC 7942DepositorInsertTransposon Tn5-692
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRL1075
Plasmid#70688PurposeUse of a vector with a conditionally lethal gene, sacB, to help transfer transposon mutations to a wild type strainDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAM2991
Plasmid#40248DepositorInsertOne Step Cloneing Vector for Overexpression
UseCyanobacteria cloning vectorTagsExpressionMutationPromotern/aAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
spoT_NS1
Plasmid#100914Purposeexpresses spoT (from native promoter) from the NS1 site in the S. elongatus PCC7942 genomeDepositorInsertsynpcc7942_1377 (spoT)
UseCyanobacteria cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
relA+_NS1
Plasmid#100916Purposeexpresses E.coli relA+ from the NS1 site in the S. elongatus PCC7942 genomeDepositorInsertRelA fragment from E.coli
UseCyanobacteria cloning vectorTagsFLAGExpressionMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEEK2
Plasmid#83492PurposeExpression plasmid for conjugational transfer into cyanobacteriaDepositorTypeEmpty backboneUseSynthetic BiologyTagsStrep-tagExpressionBacterialMutationPromoterPtrc coreAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRL271
Plasmid#70254PurposesacB plasmid with polylinker; chloramphenicol/erythromycin resistance: use as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.DepositorTypeEmpty backboneUseConditionally lethalTagsExpressionMutationPromoterAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRL277
Plasmid#70255PurposesacB plasmid with polylinker; streptomycin/spectinomycin resistance: used as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.DepositorTypeEmpty backboneUseConditionally lethalTagsExpressionMutationPromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAM1303
Plasmid#40243DepositorInsertSp/Sm Resistance, neutral site I integration platform for Synechococcus
UseCyanobacteria cloning vectorTagsExpressionMutationPromotern/aAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAM3110
Plasmid#40247DepositorInsertattP Site
UseCyanobacteria cloning vectorTagsExpressionMutationPromotern/aAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAM2314
Plasmid#40246DepositorInsertPolylinker
UseCyanobacteria cloning vectorTagsExpressionMutationPromotern/aAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
Ptrc::RpaA D53A
Plasmid#105229Purposeinducible expression of unphosphorylatable variant of RpaA (D53A)DepositorInsertrpaA driven by Ptrc promoter
UseCyanobacteria cloning vectorTagsExpressionMutationD53 mutated to APromoterptrcAvailable sinceJan. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
spoTD297A_NS1
Plasmid#100915Purposeexpresses spoTD297A (from native promoter) from the NS1 site in the S. elongatus PCC7942 genomeDepositorInsertsynpcc7942_1377 (spoT)
UseCyanobacteria cloning vectorTagsExpressionMutationChanged D 297 to APromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
relAE335Q_NS1
Plasmid#100917Purposeexpresses E.coli relAE335Q from the NS1 site in the S. elongatus PCC7942 genomeDepositorInsertRelA fragment from E.coli with point mutation E335Q
UseCyanobacteria cloning vectorTagsFLAGExpressionMutationE 335 converted to QPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
rpaA_NS1
Plasmid#101829Purposedrives expression of rpaA from the NS1 site in the S. elongatus PCC7942 genomeDepositorInsertRpaA with its native promoter
UseCyanobacteria cloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSW071
Plasmid#140037PurposePCC 11901 fadD locus KO with a kanamycin resistance cassetteDepositorInsertKanamycin resistance gene
UseGene knockout in cyanobacteriaTagsExpressionMutationPromoterKanRAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRL278
Plasmid#70256PurposesacB plasmid with polylinker; kanamycin/neomycin resistance: used as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.DepositorTypeEmpty backboneUseConditionally lethalTagsExpressionMutationPromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only