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Plasmid#160463PurposeEncodes a variant of mScarlet-I that displays reduced cytotoxicity in bacteriaDepositorInsertmScarlet-2I
UseTagsExpressionBacterialMutationPromoterT5Available sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-CI
Plasmid#51565PurposeExpression of bacteriophage lambda CI for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backboneDepositorInsertcI
UseTagsIntein-Chiting Binding DomaninExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-FIS
Plasmid#51564PurposeExpression of E. coli FIS for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backboneDepositorInsertFIS
UseTagsIntein-Chiting Binding DomaninExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-CII
Plasmid#51566PurposeExpression of bacteriophage lambda CII for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backboneDepositorInsertcII
UseTagsIntein-Chiting Binding DomaninExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT2 NCmut-msfGFP
Plasmid#180320Purposemammalian expression of human SEPT2 NCmut fused to monomeric superfolder GFPDepositorInsertSEPTIN2 (SEPTIN2 Human)
UseTagsmsfGFPExpressionMammalianMutationSEPT2 F20D, V27DPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_msfGFP-SEPT7 Gmut2
Plasmid#180425Purposemammalian expression of human SEPT7 Gmut2 fused to monomeric superfolder GFPDepositorInsertSEPTIN7 (SEPTIN7 Human)
UseTagsmsfGFPExpressionMammalianMutationSEPT7 H279DPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_msfGFP-SEPT7 Gmut1
Plasmid#180316Purposemammalian expression of human SEPT7 Gmut1 fused to monomeric superfolder GFPDepositorInsertSEPTIN7 (SEPTIN7 Human)
UseTagsmsfGFPExpressionMammalianMutationSEPT7 W269A, H279DPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJA327
Plasmid#74486Purposesuperfolder GFP (myo-3::sfGFP::let-858, using backbone as in L3785)DepositorInsertsuperfolder GFP
UseTagsExpressionWormMutationPromotermyo-3Available sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-CRP/CAP
Plasmid#51563PurposeExpression of E. coli CRP/CAP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backboneDepositorInsertCRP/CAP
UseTagsIntein-Chiting Binding DomaninExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i3 Gmut-msfGFP
Plasmid#180326Purposemammalian expression of human SEPT9_i3 Gmut fused to monomeric superfolder GFPDepositorInsertSEPTIN9_v3 (SEPTIN9 Human)
UseTagsmsfGFPExpressionMammalianMutationSEPT9_i3 W502A, H512DPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i3 NCmut-msfGFP
Plasmid#180325Purposemammalian expression of human SEPT9_i3 NCmut fused to monomeric superfolder GFPDepositorInsertSEPTIN9_v3 (SEPTIN9 Human)
UseTagsmsfGFPExpressionMammalianMutationSEPT9_i3 I263D, M270DPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
GTT-g
Plasmid#81109PurposeGene Tagging vector TRP/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)DepositorInsertsfGFP
UseCre/LoxTagssfGFPExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
GTH-g
Plasmid#81104PurposeGene Tagging vector HIS/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)DepositorInsertsfGFP
UseCre/LoxTagssfGFPExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
GTU-g
Plasmid#81094PurposeGene Tagging vector URA/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)DepositorInsertsfGFP
UseCre/LoxTagssfGFPExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
GTL-g
Plasmid#81099PurposeGene Tagging vector LEU/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)DepositorInsertsfGFP
UseCre/LoxTagssfGFPExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pnEA-vH_His-TEV-SEPT2 NCmut-msfGFP
Plasmid#180311Purposebacterial expression of human SEPT2 NCmut fused to monomeric superfolder GFPDepositorInsertSEPTIN2 (SEPTIN2 Human)
UseTagsHis6-TEV and msfGFPExpressionBacterialMutationSEPT2 F20D, V27DPromoterAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac2.0
Plasmid#119872PurposeCumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPveg promoter with CuO sequence and PxylR promoterAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac1.8
Plasmid#119871PurposeConstitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR'
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationSNP in 592 bpPromoterPserA promoter and Pveg promoter with CuO sequenceAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pS2513·PHP
Plasmid#122590PurposeConstitutive expressed pHiorine2 for pH quan. in gram-negative cellsDepositorInsertphIorine2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPem7Available sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
POLArIS_NbVim-cp10GFP
Plasmid#171020PurposeExpresses NbVim-cp10GFP#2 in mammalian cellsDepositorInsertanti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP)
UseTagscp10GFPExpressionBacterial and MammalianMutationPromoterCMV promoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only