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Showing: 221 - 240 of 367 results

  4. MBP-rPKL S113TAG

    Plasmid
    #107157
    Purpose
    MBP-rPKL S113TAG for production and purification of rat PKL pS113
    Depositor
    Jesse Rinehart
    Article
    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e
    Insert
    MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
    Use
    Tags
    C-term 6-his and N-term MBP
    Expression
    Bacterial
    Mutation
    Serine 113 changed to TAG for phosphoserine inser…
    Promoter
    PBAD
    Available since
    Dec. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    107157-plasmid-map-sequence-id-204885

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  17. pHES830

    Plasmid
    #87944
    Purpose
    Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.
    Depositor
    Hana El-Samad
    Article
    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102
    Insert
    Zif268 DBD - hPR LBD - MSN2 AD (PGR Synthetic, Budding Yeast, Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1 (crippled)
    Available since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    87944-plasmid-map-sequence-id-180365

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  18. pHES941

    Plasmid
    #87942
    Purpose
    Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Gal4 binding sites.
    Depositor
    Hana El-Samad
    Article
    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102
    Insert
    GAL4 DBD - hPR LBD - MSN2 AD (PGR Synthetic, Budding Yeast, Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    87942-plasmid-map-sequence-id-180361

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  19. pHES873

    Plasmid
    #87945
    Purpose
    Expresses estradiol-inducible synthetic transcription factor in yeast under control of GAL1 promoter. It activates transcription of promoters containing Zif268 binding sites.
    Depositor
    Hana El-Samad
    Article
    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102
    Insert
    pGAL1- Zif268 DBD - hPR LBD - MSN2 AD (PGR Budding Yeast, Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87945-plasmid-map-sequence-id-180366

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  20. pAN1646

    Plasmid
    #127205
    Purpose
    pTet expression of Venus, Dual inducible expression of dCas9-VP64-mScarlet-degronSwitchA and KeyA-BFP-NLS
    Depositor
    Hana El-Samad
    Article
    Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586
    Insert
    p7x_tet-Venus-tADH1-pGal1-dCas9-VP64-Linker-mScarlet-Linker-degronSwitch_A_t12-tPGK1-pZ3-key_A_e18-mTagBFP2-NLS(SV40)-tSSA1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    127205-plasmid-map-sequence-id-250026

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Showing: 221 - 240 of 367 results