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Showing: 221 - 240 of 77029 results

  1. lentiMutB3GNT5-blast

    Plasmid
    #208380
    Purpose
    lentiviral vector for expressing human B3GNT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence
    Depositor
    Jennifer Kohler
    Insert
    Beta-1,3-N-Acetylglucosaminyltransferase 5 (B3GNT5 Human)
    Use
    Lentiviral
    Tags
    myc, FLAG
    Expression
    Mammalian
    Mutation
    nt 922 c->g (PAM site inactivation)
    Promoter
    EF-1 alpha core promoter
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    208380-plasmid-map-sequence-id-414094

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  2. lentiMutB3GALT5-blast

    Plasmid
    #208381
    Purpose
    lentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence
    Depositor
    Jennifer Kohler
    Insert
    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    Use
    Lentiviral
    Tags
    myc, FLAG
    Expression
    Mammalian
    Mutation
    nucleotide change in PAM targeting sequence nt 51…
    Promoter
    EF-1 alpha core promoter
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    208381-plasmid-map-sequence-id-414093

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  3. lentiMutB3GALT5catdead-blast

    Plasmid
    #220869
    Purpose
    lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence
    Depositor
    Jennifer Kohler
    Insert
    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    Use
    Lentiviral
    Tags
    myc, FLAG
    Expression
    Mammalian
    Mutation
    encodes D156A and D243A mutations; also, nucleoti…
    Promoter
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220869-plasmid-map-sequence-id-440865

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  7. Plasmid EUPp_09

    Plasmid
    #157745
    Purpose
    PgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)
    Depositor
    Kang Zhou
    Article
    Liang et al Metab Eng. 2020 Nov 25. pii: S1096-7176(
    Inserts
    acetaldehyde dehydrogenase
    alcohol dehydrogenase (ADH2 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PgyrA
    Available since
    June 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    157745-plasmid-map-sequence-id-334102

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  16. CMV+ plasmid

    Plasmid
    #169740
    Purpose
    Unregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels. This plasmid has additional sequences in the UTRs to increase gene expression levels.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    eGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pCMV-tetO2
    Available since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    169740-plasmid-map-sequence-id-334212

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  20. Rlucrarr3-Flash6

    Plasmid
    #74134
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 410 of Arrestin3.
    Depositor
    Louis Luttrell
    Article
    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi
    Insert
    Arrestin3
    Use
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Promoter
    Available since
    March 25, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 221 - 240 of 77029 results