Skip to main content
Addgene
Showing: 221 - 240 of 2590 results

  2. PB_TRE_AP4-PDGFR_EF1a_eBFP2

    Plasmid
    #186306
    Purpose
    Plasmid for inducible mammalian cell expression of the AP4 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.
    Depositor
    George Church
    Article
    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01
    Inserts
    AP4-PDGFR
    eBFP2
    Use
    Tags
    HA, myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    186306-plasmid-map-sequence-id-366944

    Enlarge

  3. EBFP2-CENPB-N-22

    Plasmid
    #55237
    Purpose
    Localization: Nucleus/Centromeres, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Insert
    CENPB (CENPB Human)
    Use
    Tags
    EBFP2
    Expression
    Mammalian
    Mutation
    aa 1-169 of CENPB (DNA Binding Domain)
    Promoter
    CMV
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. pNLS-2xBFP(4C)

    Plasmid
    #176151
    Purpose
    Encodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.
    Depositor
    Siegfried Musser
    Article
    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d
    Insert
    NLS-2xBFP(4C)
    Use
    Tags
    6xHis tag and SV40 nuclear localization sequence …
    Expression
    Bacterial
    Mutation
    Compared with EGFP, the two BFP domains have the …
    Promoter
    Ptrc
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    176151-plasmid-map-sequence-id-356068

    Enlarge

  6. EBFP2-Vinculin-23

    Plasmid
    #55252
    Purpose
    Localization: Focal Adhesions, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Insert
    Vinculin (VCL Human)
    Use
    Tags
    EBFP2
    Expression
    Mammalian
    Mutation
    V234L in Vinculin
    Promoter
    CMV
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. pRVdG-4BFP2

    Plasmid
    #52490
    Purpose
    Expresses mTagBFP2
    Depositor
    Ian Wickersham
    Insert
    mTagBFP2
    Use
    Rabies virus
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    52490-plasmid-map-sequence-id-81390

    Enlarge

  8. EBFP2-Cx43

    Plasmid
    #69020
    Purpose
    Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Cx43 (Gja1 Rat)
    Use
    Tags
    EBFP2
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69020-plasmid-map-sequence-id-124710

    Enlarge

  11. EBFP2-VASP-5

    Plasmid
    #55251
    Purpose
    Localization: Actin/Focal Adhesions, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Insert
    VASP (Vasp Mouse)
    Use
    Tags
    EBFP2
    Expression
    Mammalian
    Mutation
    A209T in VASP
    Promoter
    CMV
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    55251-plasmid-map-sequence-id-212996

    Enlarge

  15. pRJPaph-eBFP2

    Plasmid
    #67030
    Purpose
    Plasmid for Paph-eBFP2 integration downstream of B. diazoefficiens (B. japonicum) scoI
    Depositor
    Julia Vorholt
    Article
    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.
    Insert
    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    Use
    Conjugative bacterial vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67030-plasmid-map-sequence-id-115538

    Enlarge

  16. EBFP2-Cx30

    Plasmid
    #69022
    Purpose
    Expresses human Cx30 (Gjb6 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Cx30 (GJB6 Human)
    Use
    Tags
    EBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69022-plasmid-map-sequence-id-127476

    Enlarge

  17. PB_TRE_sg88-PDGFR_EF1a_eBFP2

    Plasmid
    #186312
    Purpose
    Plasmid for inducible mammalian cell expression of the sg88 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.
    Depositor
    George Church
    Article
    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01
    Inserts
    sg88-PDGFR
    eBFP2
    Use
    Tags
    His, myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    186312-plasmid-map-sequence-id-366935

    Enlarge

Showing: 221 - 240 of 2590 results