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Plasmid#191327PurposeExpresses the fluorescently labeled membrane anchor C2 fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFRB-C2-dCer3
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-FRB-dCer3-CAAX
Plasmid#191326PurposeExpresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFRB-dCer-CAAX
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-TUBB2a-mEos3.2
Plasmid#191320PurposeExpresses fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV lightDepositorInsertTUBB2a-mEos3.2 (Tubb2a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-TUBB2a-Dronpa
Plasmid#191321PurposeExpresses fuorescently labeled beta tubulin to allow photoactivation of microtubule patches with very little UV lightDepositorInsertTUBB2a-Dronpa (Tubb2a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB236.1
Plasmid#170028PurposeEncodes LacI-controlled expression of surface-displayed TCP fragment lacking C-terminal alpha-helix sequence and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
TCP Δ C-terminal alpha-helix
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-Wt-PTEN
Plasmid#206982PurposeLentiviral vector for expressing doxycyclin-inducible cDNA of wild type PTENDepositorInsertPTEN (PTEN Human)
UseLentiviralTagsExpressionMammalianMutationPromotertet-on promoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-TUBB2a-mEos3.2-mEos3.2-mEos3.2
Plasmid#191338PurposeExpresses triple fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light in slice culturesDepositorInsertTUBB2a-mEos3.2-mEos3.2-mEos3.2 (Tubb2a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-NLS-PTEN
Plasmid#206984PurposeLentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with NLSDepositorInsertPTEN (PTEN Human)
UseLentiviralTagsNLSExpressionMammalianMutationPromotertet-on promoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTrc-trGPPS(CO)-LS
Plasmid#50603PurposepTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthaseDepositorInsertsLimonene Synthase
ApR
LacIq
UseSynthetic BiologyTagsExpressionBacterialMutationtruncated codon optimized geranylpyrophosphate sy…PromoterTrcAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cas12j-8-crRNA
Plasmid#194967PurposeThe plasmid expresses Cas12j-8-crRNA expression elements.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSR59.4
Plasmid#63175PurposeExpresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoterDepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2-epNTR
Plasmid#62213PurposeepNTR (zebrafish optimized nitroreductase, T41L, N71S, F124W) in pCS2DepositorInsertepNTR
UseMrna synthesisTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKB083
Plasmid#181952PurposeIPTG-inducible expression of PhoQDepositorInsertPhoQ; lacI (lacI PhoQ - Salmonella enterica subsp. enterica serovar Typhimurium; lacI - E. coli)
UseTagsExpressionBacterialMutationPromoterPhoQ-Ptac; lacI-PlacIqAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBWB419
Plasmid#140637PurposeRSF1010-Cas9-pcaHG gRNA. Vector with constitutive Cas9 with gRNA targeting pcaHG locus.DepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKMV-VioB
Plasmid#65348Purposesysthesis of vioB gene in the violacein pathwayDepositorInsertvioB
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKMV-VioD
Plasmid#65350Purposesysthesis of vioD gene in the violacein pathwayDepositorInsertvioD
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pSR83
Plasmid#90211PurposeExpresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoterDepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETM6-C4-mCherry
Plasmid#66530PurposeExpresses mCherry under the control of Mutant T7 Promoter C4DepositorInsertmCherry
UseSynthetic BiologyTagsExpressionBacterialMutationCodon Optimized for E. coliPromoterMutant T7 Promoter - C4Available sinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBWB424
Plasmid#140638PurposeRSF1010-Cas9-vanAB gRNA. Vector with constitutive Cas9 and gRNA targeting vanAB locus.DepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only