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Plasmid#191140PurposeExpression of Sensor 1 (for EK0208) in mammalian cells; mCherry marker, EGFP output.DepositorInsertmCherry:s70587:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP935-Batf3-IRES2-FlpO-WPRE-neo
Plasmid#181771Purposetargeting vectorDepositorInsertBatf3 homology arms, FlpO
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEM1 = flp-21::LoxPStopLoxP::npr-1 SL2 GFP
Plasmid#24033DepositorInsertNpr1 (npr-1 Nematode)
UseCre/LoxTagsGFPExpressionWormMutationPromoterAvailable sinceFeb. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
EK0211 CMV-TO-BFPmut-stop-t17d9b (FLP-IN)
Plasmid#191139PurposeExpression of Trigger 2 in 3' UTR of an inactive BFP in mammalian cells. Used as second input in OR, AND gatesDepositorInsertt17d9b
UseTagsExpressionMammalianMutationWTPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0294 SFFV-mCherry-SG(s70587-W)SG-EGFP (FLP-IN)
Plasmid#191141PurposePositive control for Sensor 1 (EK0285) where the TAG stop codon is replaced with TGG tryptophan codonDepositorInsertmCherry:s70587-W:EGFP
UseTagsExpressionMammalianMutationUAG>UGG (stop>Trp)PromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
NSK024 SFFV-mCherry-SG(p53R248Q.s2)SG-EGFP (FLP-IN)
Plasmid#191169PurposeExpression of a sensor for p53 mutant R248Q in mammalian cells; mCherry marker, EGFP outputDepositorInsertmCherry:p53(R248Q).s2:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0301 SFFV-mCherry-SG(Bdnf.s1)SG-EGFP (FLP-IN)
Plasmid#191144PurposeExpression of Sensor for murine Bdnf 3' UTR in mammalian cells; mCherry marker, EGFP output; 90 bp longDepositorInsertmCherry:Bdnf.s1:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0303 SFFV-mCherry-SG(Bdnf.s3)SG-EGFP (FLP-IN)
Plasmid#191180PurposeNegative control sensor in human cells.DepositorInsertmCherry:Bdnf.s3:EGFP (negative control)
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBT340_pattB-pCA-GFP-pA-FRT5-pPGK-Flpo-pA
Plasmid#52549Purposetransgenic construct for expression of GFP and FlpoDepositorInsertsPhiC31o attB (full length)
GFP
Flpo recombinase
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
EK0325 SFFV-mCherry-SG(EGFP.s2)SG-Cre (FLP-IN)
Plasmid#191160PurposeExpression of a sensor for EGFP CDS in mammalian cells; mCherry marker, Cre outputDepositorInsertmCherry:EGFP.s2:Cre
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0302 SFFV-mCherry-SG(Bdnf.s2)SG-EGFP (FLP-IN)
Plasmid#191179PurposeNegative control sensor in human cells.DepositorInsertmCherry:Bdnf.s2:EGFP (negative control)
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0304 CMV-TO-BFPmut-stop-Bdnf.t1 (FLP-IN)
Plasmid#191146PurposeExpression of only the trigger sequence for Bdnf.s1 (EK0301) in mammalian cellsDepositorInsertBdnf.t1
UseTagsExpressionMammalianMutationWTPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CB-FLPe-P2A-copGFP-T2A-Puro
Plasmid#72258PurposeExpress FLPe, copGFP and Puromycin resistance gene under CB promoterDepositorInsertFLPe-P2A-copGFp-T2A-Puro
UseLentiviralTagsExpressionMammalianMutationPromoterCB and chicken beta-actin + CMV enhancerAvailable sinceJan. 15, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOTTC1440 - pAAV CMV-IE Nuc-iRFP-2A-Flpo
Plasmid#112684PurposeAn AAV vector that expresses a nuclear-localized iRFP713 reporter and optimized Flp recombinaseDepositorInsertiRFP713
UseAAVTags2A-Flpo and Nuclear localization signalExpressionMutationPromoterCMV-IEAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EK0354 SFFV-mCherry-SG(Bdnf.s1L72)SG-EGFP (FLP-IN)
Plasmid#191151PurposeExpression of a 72 bp long sensor for murine Bdnf 3' UTR in mammalian cellsDepositorInsertmCherry:Bdnf.s1L72:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0313 SFFV-mCherry-SG(s70587)SG-Cre (FLP-IN)
Plasmid#191149PurposeExpression of Sensor 1 (for EK0208) in mammalian cells; mCherry marker, Cre output derived from a gift from prof. Liqun LuoDepositorInsertmCherry:s70587:Cre
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0261 CMV-TO-mCherry-SG(s70587)SG-fs(EGFP) (FLP-IN)
Plasmid#191175PurposeMammalian expression of EK0209 with frameshift mutation (EGFP is frameshifted).DepositorInsertCMV-TO:mCherry:s70587:EGFP (frameshifted)
UseTagsExpressionMammalianMutationEGFP is frameshiftedPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0289 SFFV-mCherry-SG(s17d9b)SG-EGFP (FLP-IN)
Plasmid#191142PurposeExpression of Sensor 2 (for EK0211) in mammalian cells; mCherry marker, EGFP output.DepositorInsertmCherry:s17d9b:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EW172 CMV-TO-UTRN-14ZF-VP64 (FLP-IN)
Plasmid#236160PurposePlasmid encoding the UTRN-14 zinc finger array attached by a GS linker to the VP64 transcriptional activation domain, under control of CMV promoter with two TetR binding sitesDepositorInsertUTRN-14ZF-VP64
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW215 PGK-ZNF35(5-8)-deImmunLink-NZF(FLP-IN)
Plasmid#236130PurposePlasmid encoding the ZNF35-derived portion of the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of PGK promoterDepositorInsertZNF35(5-8)-deImmunLink-NZF
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW269 CMV-TO-Gal4-NZF[GS Linkers] (FLP-IN)
Plasmid#236122PurposePlasmid encoding Gal4-NZF transcription factor with GS linkers between the component domains of NZF, under control of CMV promoter with two TetR binding sitesDepositorInsertGal4-NZF (GS Linkers)
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW216 PGK-ZNF250(1-4)-deImmunLink-NZF(FLP-IN)
Plasmid#236131PurposePlasmid encoding the ZNF250-derived portion of the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of PGK promoterDepositorInsertZNF250(1-4)-deImmunLink-NZF
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW171 CMV-TO-UTRN-43ZF-VP64 (FLP-IN)
Plasmid#236161PurposePlasmid encoding the UTRN-43 zinc finger array attached by a GS linker to the VP64 transcriptional activation domain, under control of CMV promoter with two TetR binding sitesDepositorInsertUTRN-43ZF-VP64
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW446 CMV-TO-ADAR2dd(E488Q, T501A) (FLP-IN)
Plasmid#236125PurposePlasmid encoding free ADAR2dd under control of CMV promoter with two TetR binding sitesDepositorInsertADAR2dd (ADARB1 Human, Synthetic)
UseTagsExpressionMammalianMutationE488Q, T501APromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW418 PGK-IKZF1(2-3)-realDeimmunLink-NZF (FLP-IN)
Plasmid#236155PurposePlasmid encoding the IKZF1-derived portion of the ZNF35/IKZF1 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of PGK promoterDepositorInsertIKZF1(2-3)-deimmunLink-NZF
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW368 CMV-TO-UtroUpZF-deImmunLink-NZF(FLP-IN)
Plasmid#236154PurposePlasmid encoding the UtroUp zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sitesDepositorInsertUtroUpZF-deImmunLink-NZF
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Esyn-FlpX-TVA950-EYFP-WPRE
Plasmid#234710PurposepAAV transfer plasmid with enhanced Synapsin promoter driving Flp-dependent expression of TVA950-eYFPDepositorInsertTVA950-eYFP
UseAAV; Has fdio cassette for flp-dependent expressi…TagsExpressionMammalianMutationPromoterenhanced SynapsinAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP1633 - pAAV-AiE2114m-minBG-FlpO-HGHpA
Plasmid#230678PurposeAiE2114m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cellsInsertFlpO
UseAAVTagsExpressionMutationPromoterminBGAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP1537 - pAAV-AiE2135m_3xC2-minBG-FlpO-HGHpA
Plasmid#220709PurposeAiE2135m_3xC2 is an optimized enhancer sequence, designed to induce Flp-dependent recombination in specific populations of brain cellsInsertFlpO
UseAAVTagsExpressionMutationPromoterminBGAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
EK0209 CMV-TO-mCherry-SG(s70587)SG-EGFP (FLP-IN)
Plasmid#191174PurposeMammalian expression of EK0285 with CMV-tetO promoter instead of SFFV.DepositorInsertCMV-TO:mCherry:s70587:EGFP
UseTagsExpressionMammalianMutationWTPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0344 SFFV-mCherry-SG(Bdnf.s1)SG-Cre (FLP-IN)
Plasmid#191158PurposeExpression of a sensor for murine Bdnf 3' UTR in mammalian cells; mCherry marker, Cre output; 90 bp longDepositorInsertmCherry:Bdnf.s1:Cre
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0441 CMV-TO-BFPmut-stop-EGFP.t2 (FLP-IN)
Plasmid#191161PurposeExpression of a trigger sequence from EGFP CDS in 3' UTR context in mammalian cellsDepositorInsertEGFP.t2
UseTagsExpressionMammalianMutationWTPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0341 SFFV-mCherry-SG(Bdnf.s1L153)SG-EGFP (FLP-IN)
Plasmid#191150PurposeExpression of a 153 bp long sensor for murine Bdnf 3' UTR in mammalian cellsDepositorInsertmCherry:Bdnf.s1L153:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0358 SFFV-mCherry-SG(Bdnf.s1L36)SG-EGFP (FLP-IN)
Plasmid#191152PurposeExpression of a 36 bp long sensor for murine Bdnf 3' UTR in mammalian cellsDepositorInsertmCherry:Bdnf.s1L36:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP949-Slc17a7-IRES2-FlpO-WPRE-neo
Plasmid#181776Purposetargeting vectorDepositorInsertSlc17a7 homology arms, FlpO
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP937-Gpr139-IRES2-FlpO-WPRE-neo
Plasmid#181768Purposetargeting vectorDepositorInsertGpr139 homology arms, FlpO
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP936-Chrna6-IRES2-FlpO-WPRE-neo
Plasmid#181766Purposetargeting vectorDepositorInsertChrna6 homology arms, FlpO
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0498 SFFV-mCherry-SGc(ACTB.s1)cSG-EGFP-(3xMS2) (FLP-IN)
Plasmid#191182PurposeExpression of EACTB sensor in mammalian cellsDepositorInsertmCherry:ACTB.s1:EGFP-3xMS2
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0493 SFFV-mCherry-SGc(GAPDH.s1)cSG-EGFP-(3xMS2) (FLP-IN)
Plasmid#191156PurposeExpression of a sensor for GAPDH 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTRDepositorInsertmCherry:GAPDH.s1:EGFP-3xMS2
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0438 SFFV-mCherry-SGc(s70587)cSG-EGFP-(3xMS2) (FLP-IN)
Plasmid#191163PurposeExpression of a sensor for synthetic sequence 1 (EK0208) with three MS2 sequences in the 3' UTR in mammalian cells. Compared to EK0285, also has additional restriction sites around the sensor sequence. Recommended for the basis for cloning new sensors for use with MCP-ADAR(DD). HindIII & MluI can be used to swap out marker (mCherry). (KpnI or BamHI) and (BshTI or BcuI) can be used to swap out sensor sequence (s70587). XhoI and ApaI can be used to swap out output (EGFP).DepositorInsertmCherry:s70587:EGFP-3xMS2
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only