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Plasmid#131787PurposeTol2 bicistronic vector expressing PcyA and HO1 for Phycocyanobilin (PCB) synthesis, driven by heatshock promoter hsp70.DepositorInsertPcyA and HO1
UseSynthetic Biology; Zebrafish tol2 transposonTagsMTS (Mitochondrial Targeting Signal from subunit …ExpressionMammalianMutationPromoterhsp70Available sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Danio alpha Bb-crystallin 1kb promoter/AcGFP
Plasmid#98098PurposeaBb-crystallin promoter fragment in GFP plasmid to assess activity in larval embryosDepositorInsertAlpha Bb-crystallin promoter 1 kb fragment, Danio rerio (cryabb Zebrafish)
UseGfp expressingTagsGFPExpressionMutationPromoterDanio aBb-crystallin 1 kb fragment (-1074/-1)Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMini-CAAGs::RFP-DR48
Plasmid#132640PurposeFluorescent reporter to quantify elicitation of the strand annealing mediated repair pathwayDepositorInsertmRFP
UseTagsExpressionMammalianMutationContains a 92bp insertion interrupting the open …PromoterChicken B-actinAvailable sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-mVenus1-155-GGGS
Plasmid#162610PurposeAllows for transcription of control mVenus fluorophore half for injection into zebrafish embryos for BiFC assaysDepositorInsertmVenus
UseTagsExpressionMammalianMutationContains aa1-155 of mVenusPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
FnoCas12a-DR-GC-swap-cr in pBCSK+
Plasmid#126644PurposeCloning vector FnoCas12a-DR-GC-swap-crRNA for expression in mammalian cellsDepositorInsertFnoCas12a Full Length Direct Repeat GC Swap crRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6 PromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF
Plasmid#79889PurposeDonor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertHA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEM BirA-2A-Citrine-SV40pA-FRT-Kan-FRT
Plasmid#89890PurposeBAC donor construct containing 3XHA-tagged BirA, a ribosome skipping motif - 2A, Citrine reporter, polyadenyation signal, followed by FRT recombination sites flanking kanamycin selection cassette.DepositorInsertHA-BirA-2A-citrine
UseUnspecifiedTagsCitrineExpressionMutationPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-ARF1-DN
Plasmid#109536PurposeMuscle specific zebrafish expression of mKate2 fused to dominant negative ARF1DepositorInsertARF1 (arf1 Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationDNPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Danio alpha Ba-crystallin 3kb promoter/AcGFP
Plasmid#98097PurposeaBA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryosDepositorInsertAlpha Ba-crystallin promoter 3 kb fragment, Danio rerio (cryaba Zebrafish)
UseGfp expressingTagsGFPExpressionMutationPromoterDanio alpha Ba-crystallin 3 kb fragment (-3000/-1)Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-ARF6-DN
Plasmid#109538PurposeMuscle specific zebrafish expression of mKate2 fused to dominant negative ARF6DepositorInsertARF6 (arf6a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationDNPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_+15.2prox1a:EGFP_XCA:DsRed2
Plasmid#218206PurposeZebrafish +15.2prox1a enhancer reporter in the FCLV from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert+15.2prox1a
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2
Plasmid#218207PurposeZebrafish -2.1prox1a_1 (mouse conserved) enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert-2.1prox1a_1
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_-2.1prox1a_2:EGFP_XCA:DsRed2
Plasmid#218208PurposeZebrafish -2.1prox1a_2 (not mouse conserved) enhancer reporter in the facial lymphatics at 5 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert-2.1prox1a_2
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2
Plasmid#218205PurposeZebrafish -2.1prox1a enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert-2.1prox1a
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
ACVR1-TASA-R206H
Plasmid#206182Purposein vitro transcription of RNA encoding human ACVR1 with mutations S190A, S194A, and R206HDepositorInsertactivin A receptor type 1 (ACVR1 Human)
UseIn vitro transcriptionTagsFLAG tagExpressionMutationS190A, S194A, R206HPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
ACVR1-TSAA-R206H
Plasmid#206183Purposein vitro transcription of RNA encoding human ACVR1 with mutations S192A, S194A, and R206HDepositorInsertactivin A receptor type 1 (ACVR1 Human)
UseIn vitro transcriptionTagsFLAG tagExpressionMutationS192A, S194A, R206HPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
ACVR1-VSAS-R206H
Plasmid#206185Purposein vitro transcription of RNA encoding human ACVR1 with mutations T189V, S192A, and R206HDepositorInsertactivin A receptor type 1 (ACVR1 Human)
UseIn vitro transcriptionTagsFLAG tagExpressionMutationT189V, S192A, R206HPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
ACVR1-VASS-R206H
Plasmid#206186Purposein vitro transcription of RNA encoding human ACVR1 with mutations T189V, S190A, and R206HDepositorInsertactivin A receptor type 1 (ACVR1 Human)
UseIn vitro transcriptionTagsFLAG tagExpressionMutationT189V, S190A, R206HPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
ND2-FusXTBE-Left TALE
Plasmid#196498PurposeExpresses left-side TALE-DddAtox Cterm half ND2-FusXTBE in mammalian cellsDepositorInsertCOX8A MTS-ND2 left TALE-G1397 DddA-C-UGI-ATP5B 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
ND2-FusXTBE-Right TALE
Plasmid#196499PurposeExpresses Right-side TALE-DddAtox Nterm half ND2-FusXTBE in mammalian cellsDepositorInsertCOX8A MTS-ND2 right TALE-G1397 DddA-C-UGI-ATP5B 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only